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A Simple and Cost-Efficient Platform for a Novel Porcine Circovirus Type 2d (PCV2d) Vaccine Manufacturing

Porcine circovirus type 2d (PCV2d) is becoming the predominant PCV genotype and considerably affects the global pig industry. Nevertheless, currently, no commercial PCV2d vaccine is available. Preventing and controlling the disease caused by PCV2d is therefore based on other genotype-based vaccines....

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Autores principales: Noppiboon, Sarawuth, Lapanusorn, Neeracha, Ekkpongpaisit, Pisit, Slack, Sarah, Frank, Stefanie, Hocharoen, Lalintip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865830/
https://www.ncbi.nlm.nih.gov/pubmed/36680014
http://dx.doi.org/10.3390/vaccines11010169
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author Noppiboon, Sarawuth
Lapanusorn, Neeracha
Ekkpongpaisit, Pisit
Slack, Sarah
Frank, Stefanie
Hocharoen, Lalintip
author_facet Noppiboon, Sarawuth
Lapanusorn, Neeracha
Ekkpongpaisit, Pisit
Slack, Sarah
Frank, Stefanie
Hocharoen, Lalintip
author_sort Noppiboon, Sarawuth
collection PubMed
description Porcine circovirus type 2d (PCV2d) is becoming the predominant PCV genotype and considerably affects the global pig industry. Nevertheless, currently, no commercial PCV2d vaccine is available. Preventing and controlling the disease caused by PCV2d is therefore based on other genotype-based vaccines. However, their production platforms are laborious, limited in expression level, and relatively expensive for veterinary applications. To address these challenges, we have developed a simple and cost-efficient platform for a novel PCV2d vaccine production process, using fed-batch E. coli fermentation followed by cell disruption and filtration, and a single purification step via cation exchange chromatography. The process was developed at bench scale and then pilot scale, where the PCV2d subunit protein yield was approximately 0.93 g/L fermentation volume in a short production time. Moreover, we have successfully implemented this production process at two different sites, in Southeast Asia and Europe. This demonstrates transferability and the high potential for successful industrial production.
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spelling pubmed-98658302023-01-22 A Simple and Cost-Efficient Platform for a Novel Porcine Circovirus Type 2d (PCV2d) Vaccine Manufacturing Noppiboon, Sarawuth Lapanusorn, Neeracha Ekkpongpaisit, Pisit Slack, Sarah Frank, Stefanie Hocharoen, Lalintip Vaccines (Basel) Article Porcine circovirus type 2d (PCV2d) is becoming the predominant PCV genotype and considerably affects the global pig industry. Nevertheless, currently, no commercial PCV2d vaccine is available. Preventing and controlling the disease caused by PCV2d is therefore based on other genotype-based vaccines. However, their production platforms are laborious, limited in expression level, and relatively expensive for veterinary applications. To address these challenges, we have developed a simple and cost-efficient platform for a novel PCV2d vaccine production process, using fed-batch E. coli fermentation followed by cell disruption and filtration, and a single purification step via cation exchange chromatography. The process was developed at bench scale and then pilot scale, where the PCV2d subunit protein yield was approximately 0.93 g/L fermentation volume in a short production time. Moreover, we have successfully implemented this production process at two different sites, in Southeast Asia and Europe. This demonstrates transferability and the high potential for successful industrial production. MDPI 2023-01-12 /pmc/articles/PMC9865830/ /pubmed/36680014 http://dx.doi.org/10.3390/vaccines11010169 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Noppiboon, Sarawuth
Lapanusorn, Neeracha
Ekkpongpaisit, Pisit
Slack, Sarah
Frank, Stefanie
Hocharoen, Lalintip
A Simple and Cost-Efficient Platform for a Novel Porcine Circovirus Type 2d (PCV2d) Vaccine Manufacturing
title A Simple and Cost-Efficient Platform for a Novel Porcine Circovirus Type 2d (PCV2d) Vaccine Manufacturing
title_full A Simple and Cost-Efficient Platform for a Novel Porcine Circovirus Type 2d (PCV2d) Vaccine Manufacturing
title_fullStr A Simple and Cost-Efficient Platform for a Novel Porcine Circovirus Type 2d (PCV2d) Vaccine Manufacturing
title_full_unstemmed A Simple and Cost-Efficient Platform for a Novel Porcine Circovirus Type 2d (PCV2d) Vaccine Manufacturing
title_short A Simple and Cost-Efficient Platform for a Novel Porcine Circovirus Type 2d (PCV2d) Vaccine Manufacturing
title_sort simple and cost-efficient platform for a novel porcine circovirus type 2d (pcv2d) vaccine manufacturing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865830/
https://www.ncbi.nlm.nih.gov/pubmed/36680014
http://dx.doi.org/10.3390/vaccines11010169
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