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Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water

Evaluating potential environmental and clinical impacts of industrial antibiotic use is critical in mitigating the spread of antimicrobial resistance. Using soil columns to simulate field application of swine or cattle manure and subsequent rain events, and a targeted qPCR-based approach, we tracked...

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Autores principales: Colgan, Phil, Rieke, Elizabeth L., Nadeem, Khurram, Moorman, Thomas B., Soupir, Michelle L., Howe, Adina, Ricker, Nicole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865870/
https://www.ncbi.nlm.nih.gov/pubmed/36677309
http://dx.doi.org/10.3390/microorganisms11010017
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author Colgan, Phil
Rieke, Elizabeth L.
Nadeem, Khurram
Moorman, Thomas B.
Soupir, Michelle L.
Howe, Adina
Ricker, Nicole
author_facet Colgan, Phil
Rieke, Elizabeth L.
Nadeem, Khurram
Moorman, Thomas B.
Soupir, Michelle L.
Howe, Adina
Ricker, Nicole
author_sort Colgan, Phil
collection PubMed
description Evaluating potential environmental and clinical impacts of industrial antibiotic use is critical in mitigating the spread of antimicrobial resistance. Using soil columns to simulate field application of swine or cattle manure and subsequent rain events, and a targeted qPCR-based approach, we tracked resistance genes from source manures and identified important differences in antimicrobial resistance gene transport and enrichment over time in the soil and water of artificially drained cropland. The source manures had distinct microbial community and resistance gene profiles, and these differences were also reflected in the soil columns after manure application. Antibiotic resistance genes (ARGs) were only significantly enriched in effluent samples following the first rain event (day 11) for both soil types compared to the control columns, illustrating the high background level of resistance present in the control soils chosen. For swine, the genes tetQ, tet(36), tet44, tetM, sul2 and ant(6)-ib persisted in the soil columns, whereas tetO, strB and sul1 persisted in effluent samples. Conversely, for cattle manure sul2 and strB persisted in both soil and effluent. The distinct temporal dynamics of ARG distribution between soil and effluent water for each manure type can be used to inform potential mitigation strategies in the future.
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spelling pubmed-98658702023-01-22 Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water Colgan, Phil Rieke, Elizabeth L. Nadeem, Khurram Moorman, Thomas B. Soupir, Michelle L. Howe, Adina Ricker, Nicole Microorganisms Article Evaluating potential environmental and clinical impacts of industrial antibiotic use is critical in mitigating the spread of antimicrobial resistance. Using soil columns to simulate field application of swine or cattle manure and subsequent rain events, and a targeted qPCR-based approach, we tracked resistance genes from source manures and identified important differences in antimicrobial resistance gene transport and enrichment over time in the soil and water of artificially drained cropland. The source manures had distinct microbial community and resistance gene profiles, and these differences were also reflected in the soil columns after manure application. Antibiotic resistance genes (ARGs) were only significantly enriched in effluent samples following the first rain event (day 11) for both soil types compared to the control columns, illustrating the high background level of resistance present in the control soils chosen. For swine, the genes tetQ, tet(36), tet44, tetM, sul2 and ant(6)-ib persisted in the soil columns, whereas tetO, strB and sul1 persisted in effluent samples. Conversely, for cattle manure sul2 and strB persisted in both soil and effluent. The distinct temporal dynamics of ARG distribution between soil and effluent water for each manure type can be used to inform potential mitigation strategies in the future. MDPI 2022-12-21 /pmc/articles/PMC9865870/ /pubmed/36677309 http://dx.doi.org/10.3390/microorganisms11010017 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Colgan, Phil
Rieke, Elizabeth L.
Nadeem, Khurram
Moorman, Thomas B.
Soupir, Michelle L.
Howe, Adina
Ricker, Nicole
Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water
title Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water
title_full Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water
title_fullStr Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water
title_full_unstemmed Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water
title_short Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water
title_sort impact of swine and cattle manure treatment on the microbial composition and resistome of soil and drainage water
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865870/
https://www.ncbi.nlm.nih.gov/pubmed/36677309
http://dx.doi.org/10.3390/microorganisms11010017
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