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Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water
Evaluating potential environmental and clinical impacts of industrial antibiotic use is critical in mitigating the spread of antimicrobial resistance. Using soil columns to simulate field application of swine or cattle manure and subsequent rain events, and a targeted qPCR-based approach, we tracked...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865870/ https://www.ncbi.nlm.nih.gov/pubmed/36677309 http://dx.doi.org/10.3390/microorganisms11010017 |
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author | Colgan, Phil Rieke, Elizabeth L. Nadeem, Khurram Moorman, Thomas B. Soupir, Michelle L. Howe, Adina Ricker, Nicole |
author_facet | Colgan, Phil Rieke, Elizabeth L. Nadeem, Khurram Moorman, Thomas B. Soupir, Michelle L. Howe, Adina Ricker, Nicole |
author_sort | Colgan, Phil |
collection | PubMed |
description | Evaluating potential environmental and clinical impacts of industrial antibiotic use is critical in mitigating the spread of antimicrobial resistance. Using soil columns to simulate field application of swine or cattle manure and subsequent rain events, and a targeted qPCR-based approach, we tracked resistance genes from source manures and identified important differences in antimicrobial resistance gene transport and enrichment over time in the soil and water of artificially drained cropland. The source manures had distinct microbial community and resistance gene profiles, and these differences were also reflected in the soil columns after manure application. Antibiotic resistance genes (ARGs) were only significantly enriched in effluent samples following the first rain event (day 11) for both soil types compared to the control columns, illustrating the high background level of resistance present in the control soils chosen. For swine, the genes tetQ, tet(36), tet44, tetM, sul2 and ant(6)-ib persisted in the soil columns, whereas tetO, strB and sul1 persisted in effluent samples. Conversely, for cattle manure sul2 and strB persisted in both soil and effluent. The distinct temporal dynamics of ARG distribution between soil and effluent water for each manure type can be used to inform potential mitigation strategies in the future. |
format | Online Article Text |
id | pubmed-9865870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98658702023-01-22 Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water Colgan, Phil Rieke, Elizabeth L. Nadeem, Khurram Moorman, Thomas B. Soupir, Michelle L. Howe, Adina Ricker, Nicole Microorganisms Article Evaluating potential environmental and clinical impacts of industrial antibiotic use is critical in mitigating the spread of antimicrobial resistance. Using soil columns to simulate field application of swine or cattle manure and subsequent rain events, and a targeted qPCR-based approach, we tracked resistance genes from source manures and identified important differences in antimicrobial resistance gene transport and enrichment over time in the soil and water of artificially drained cropland. The source manures had distinct microbial community and resistance gene profiles, and these differences were also reflected in the soil columns after manure application. Antibiotic resistance genes (ARGs) were only significantly enriched in effluent samples following the first rain event (day 11) for both soil types compared to the control columns, illustrating the high background level of resistance present in the control soils chosen. For swine, the genes tetQ, tet(36), tet44, tetM, sul2 and ant(6)-ib persisted in the soil columns, whereas tetO, strB and sul1 persisted in effluent samples. Conversely, for cattle manure sul2 and strB persisted in both soil and effluent. The distinct temporal dynamics of ARG distribution between soil and effluent water for each manure type can be used to inform potential mitigation strategies in the future. MDPI 2022-12-21 /pmc/articles/PMC9865870/ /pubmed/36677309 http://dx.doi.org/10.3390/microorganisms11010017 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Colgan, Phil Rieke, Elizabeth L. Nadeem, Khurram Moorman, Thomas B. Soupir, Michelle L. Howe, Adina Ricker, Nicole Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water |
title | Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water |
title_full | Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water |
title_fullStr | Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water |
title_full_unstemmed | Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water |
title_short | Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water |
title_sort | impact of swine and cattle manure treatment on the microbial composition and resistome of soil and drainage water |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865870/ https://www.ncbi.nlm.nih.gov/pubmed/36677309 http://dx.doi.org/10.3390/microorganisms11010017 |
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