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Non-Destructive Direct Pericarp Thickness Measurement of Sorghum Kernels Using Extended-Focus Optical Coherence Microscopy

Non-destructive measurements of internal morphological structures in plant materials such as seeds are of high interest in agricultural research. The estimation of pericarp thickness is important to understand the grain quality and storage stability of seeds and can play a crucial role in improving...

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Autores principales: Sen, Dipankar, Fernández, Alma, Crozier, Daniel, Henrich, Brian, Sokolov, Alexei V., Scully, Marlan O., Rooney, William L., Verhoef, Aart J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865951/
https://www.ncbi.nlm.nih.gov/pubmed/36679502
http://dx.doi.org/10.3390/s23020707
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author Sen, Dipankar
Fernández, Alma
Crozier, Daniel
Henrich, Brian
Sokolov, Alexei V.
Scully, Marlan O.
Rooney, William L.
Verhoef, Aart J.
author_facet Sen, Dipankar
Fernández, Alma
Crozier, Daniel
Henrich, Brian
Sokolov, Alexei V.
Scully, Marlan O.
Rooney, William L.
Verhoef, Aart J.
author_sort Sen, Dipankar
collection PubMed
description Non-destructive measurements of internal morphological structures in plant materials such as seeds are of high interest in agricultural research. The estimation of pericarp thickness is important to understand the grain quality and storage stability of seeds and can play a crucial role in improving crop yield. In this study, we demonstrate the applicability of fiber-based Bessel beam Fourier domain (FD) optical coherence microscopy (OCM) with a nearly constant high lateral resolution maintained at over ~400 µm for direct non-invasive measurement of the pericarp thickness of two different sorghum genotypes. Whereas measurements based on axial profiles need additional knowledge of the pericarp refractive index, en-face views allow for direct distance measurements. We directly determine pericarp thickness from lateral sections with a 3 µm resolution by taking the width of the signal corresponding to the pericarp at the 1/e threshold. These measurements enable differentiation of the two genotypes with 100% accuracy. We find that trading image resolution for acquisition speed and view size reduces the classification accuracy. Average pericarp thicknesses of 74 µm (thick phenotype) and 43 µm (thin phenotype) are obtained from high-resolution lateral sections, and are in good agreement with previously reported measurements of the same genotypes. Extracting the morphological features of plant seeds using Bessel beam FD-OCM is expected to provide valuable information to the food processing industry and plant breeding programs.
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spelling pubmed-98659512023-01-22 Non-Destructive Direct Pericarp Thickness Measurement of Sorghum Kernels Using Extended-Focus Optical Coherence Microscopy Sen, Dipankar Fernández, Alma Crozier, Daniel Henrich, Brian Sokolov, Alexei V. Scully, Marlan O. Rooney, William L. Verhoef, Aart J. Sensors (Basel) Article Non-destructive measurements of internal morphological structures in plant materials such as seeds are of high interest in agricultural research. The estimation of pericarp thickness is important to understand the grain quality and storage stability of seeds and can play a crucial role in improving crop yield. In this study, we demonstrate the applicability of fiber-based Bessel beam Fourier domain (FD) optical coherence microscopy (OCM) with a nearly constant high lateral resolution maintained at over ~400 µm for direct non-invasive measurement of the pericarp thickness of two different sorghum genotypes. Whereas measurements based on axial profiles need additional knowledge of the pericarp refractive index, en-face views allow for direct distance measurements. We directly determine pericarp thickness from lateral sections with a 3 µm resolution by taking the width of the signal corresponding to the pericarp at the 1/e threshold. These measurements enable differentiation of the two genotypes with 100% accuracy. We find that trading image resolution for acquisition speed and view size reduces the classification accuracy. Average pericarp thicknesses of 74 µm (thick phenotype) and 43 µm (thin phenotype) are obtained from high-resolution lateral sections, and are in good agreement with previously reported measurements of the same genotypes. Extracting the morphological features of plant seeds using Bessel beam FD-OCM is expected to provide valuable information to the food processing industry and plant breeding programs. MDPI 2023-01-08 /pmc/articles/PMC9865951/ /pubmed/36679502 http://dx.doi.org/10.3390/s23020707 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sen, Dipankar
Fernández, Alma
Crozier, Daniel
Henrich, Brian
Sokolov, Alexei V.
Scully, Marlan O.
Rooney, William L.
Verhoef, Aart J.
Non-Destructive Direct Pericarp Thickness Measurement of Sorghum Kernels Using Extended-Focus Optical Coherence Microscopy
title Non-Destructive Direct Pericarp Thickness Measurement of Sorghum Kernels Using Extended-Focus Optical Coherence Microscopy
title_full Non-Destructive Direct Pericarp Thickness Measurement of Sorghum Kernels Using Extended-Focus Optical Coherence Microscopy
title_fullStr Non-Destructive Direct Pericarp Thickness Measurement of Sorghum Kernels Using Extended-Focus Optical Coherence Microscopy
title_full_unstemmed Non-Destructive Direct Pericarp Thickness Measurement of Sorghum Kernels Using Extended-Focus Optical Coherence Microscopy
title_short Non-Destructive Direct Pericarp Thickness Measurement of Sorghum Kernels Using Extended-Focus Optical Coherence Microscopy
title_sort non-destructive direct pericarp thickness measurement of sorghum kernels using extended-focus optical coherence microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865951/
https://www.ncbi.nlm.nih.gov/pubmed/36679502
http://dx.doi.org/10.3390/s23020707
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