Cargando…
siRNA-Mediated Timp1 Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury
Acute lung injury is a complex cascade process that develops in response to various damaging factors, which can lead to acute respiratory distress syndrome. Within this study, based on bioinformatics reanalysis of available full-transcriptome data of acute lung injury induced in mice and humans by v...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865963/ https://www.ncbi.nlm.nih.gov/pubmed/36675165 http://dx.doi.org/10.3390/ijms24021641 |
_version_ | 1784875970386198528 |
---|---|
author | Chernikov, Ivan V. Staroseletz, Yaroslav Yu. Tatarnikova, Irina S. Sen’kova, Aleksandra V. Savin, Innokenty A. Markov, Andrey V. Logashenko, Evgeniya B. Chernolovskaya, Elena L. Zenkova, Marina A. Vlassov, Valentin V. |
author_facet | Chernikov, Ivan V. Staroseletz, Yaroslav Yu. Tatarnikova, Irina S. Sen’kova, Aleksandra V. Savin, Innokenty A. Markov, Andrey V. Logashenko, Evgeniya B. Chernolovskaya, Elena L. Zenkova, Marina A. Vlassov, Valentin V. |
author_sort | Chernikov, Ivan V. |
collection | PubMed |
description | Acute lung injury is a complex cascade process that develops in response to various damaging factors, which can lead to acute respiratory distress syndrome. Within this study, based on bioinformatics reanalysis of available full-transcriptome data of acute lung injury induced in mice and humans by various factors, we selected a set of genes that could serve as good targets for suppressing inflammation in the lung tissue, evaluated their expression in the cells of different origins during LPS-induced inflammation, and chose the tissue inhibitor of metalloproteinase Timp1 as a promising target for suppressing inflammation. We designed an effective chemically modified anti-TIMP1 siRNA and showed that Timp1 silencing correlates with a decrease in the pro-inflammatory cytokine IL6 secretion in cultured macrophage cells and reduces the severity of LPS-induced acute lung injury in a mouse model. |
format | Online Article Text |
id | pubmed-9865963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98659632023-01-22 siRNA-Mediated Timp1 Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury Chernikov, Ivan V. Staroseletz, Yaroslav Yu. Tatarnikova, Irina S. Sen’kova, Aleksandra V. Savin, Innokenty A. Markov, Andrey V. Logashenko, Evgeniya B. Chernolovskaya, Elena L. Zenkova, Marina A. Vlassov, Valentin V. Int J Mol Sci Article Acute lung injury is a complex cascade process that develops in response to various damaging factors, which can lead to acute respiratory distress syndrome. Within this study, based on bioinformatics reanalysis of available full-transcriptome data of acute lung injury induced in mice and humans by various factors, we selected a set of genes that could serve as good targets for suppressing inflammation in the lung tissue, evaluated their expression in the cells of different origins during LPS-induced inflammation, and chose the tissue inhibitor of metalloproteinase Timp1 as a promising target for suppressing inflammation. We designed an effective chemically modified anti-TIMP1 siRNA and showed that Timp1 silencing correlates with a decrease in the pro-inflammatory cytokine IL6 secretion in cultured macrophage cells and reduces the severity of LPS-induced acute lung injury in a mouse model. MDPI 2023-01-13 /pmc/articles/PMC9865963/ /pubmed/36675165 http://dx.doi.org/10.3390/ijms24021641 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chernikov, Ivan V. Staroseletz, Yaroslav Yu. Tatarnikova, Irina S. Sen’kova, Aleksandra V. Savin, Innokenty A. Markov, Andrey V. Logashenko, Evgeniya B. Chernolovskaya, Elena L. Zenkova, Marina A. Vlassov, Valentin V. siRNA-Mediated Timp1 Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury |
title | siRNA-Mediated Timp1 Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury |
title_full | siRNA-Mediated Timp1 Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury |
title_fullStr | siRNA-Mediated Timp1 Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury |
title_full_unstemmed | siRNA-Mediated Timp1 Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury |
title_short | siRNA-Mediated Timp1 Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury |
title_sort | sirna-mediated timp1 silencing inhibited the inflammatory phenotype during acute lung injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865963/ https://www.ncbi.nlm.nih.gov/pubmed/36675165 http://dx.doi.org/10.3390/ijms24021641 |
work_keys_str_mv | AT chernikovivanv sirnamediatedtimp1silencinginhibitedtheinflammatoryphenotypeduringacutelunginjury AT staroseletzyaroslavyu sirnamediatedtimp1silencinginhibitedtheinflammatoryphenotypeduringacutelunginjury AT tatarnikovairinas sirnamediatedtimp1silencinginhibitedtheinflammatoryphenotypeduringacutelunginjury AT senkovaaleksandrav sirnamediatedtimp1silencinginhibitedtheinflammatoryphenotypeduringacutelunginjury AT savininnokentya sirnamediatedtimp1silencinginhibitedtheinflammatoryphenotypeduringacutelunginjury AT markovandreyv sirnamediatedtimp1silencinginhibitedtheinflammatoryphenotypeduringacutelunginjury AT logashenkoevgeniyab sirnamediatedtimp1silencinginhibitedtheinflammatoryphenotypeduringacutelunginjury AT chernolovskayaelenal sirnamediatedtimp1silencinginhibitedtheinflammatoryphenotypeduringacutelunginjury AT zenkovamarinaa sirnamediatedtimp1silencinginhibitedtheinflammatoryphenotypeduringacutelunginjury AT vlassovvalentinv sirnamediatedtimp1silencinginhibitedtheinflammatoryphenotypeduringacutelunginjury |