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Identification of Nanoparticle Properties for Optimal Drug Delivery across a Physiological Cell Barrier
Nanoparticles (NPs) represent an attractive strategy to overcome difficulties associated with the delivery of therapeutics. Knowing the optimal properties of NPs to address these issues could allow for improved in vivo responses. This work investigated NPs prepared from 5 materials of 3 sizes and 3...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865979/ https://www.ncbi.nlm.nih.gov/pubmed/36678829 http://dx.doi.org/10.3390/pharmaceutics15010200 |
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author | Ross, Aisling M. Cahalane, Rachel M. Walsh, Darragh R. Grabrucker, Andreas M. Marcar, Lynnette Mulvihill, John J. E. |
author_facet | Ross, Aisling M. Cahalane, Rachel M. Walsh, Darragh R. Grabrucker, Andreas M. Marcar, Lynnette Mulvihill, John J. E. |
author_sort | Ross, Aisling M. |
collection | PubMed |
description | Nanoparticles (NPs) represent an attractive strategy to overcome difficulties associated with the delivery of therapeutics. Knowing the optimal properties of NPs to address these issues could allow for improved in vivo responses. This work investigated NPs prepared from 5 materials of 3 sizes and 3 concentrations applied to a cell barrier model. The NPs permeability across a cell barrier and their effects on cell barrier integrity and cell viability were evaluated. The properties of these NPs, as determined in water (traditional) vs. media (realistic), were compared to cell responses. It was found that for all cellular activities, NP properties determined in media was the best predictor of the cell response. Notably, ZnO NPs caused significant alterations to cell viability across all 3 cell lines tested. Importantly, we report that the zeta potential of NPs correlates significantly with NP permeability and NP-induced changes in cell viability. NPs with physiological-based zeta potential of −12 mV result in good cell barrier penetration without considerable changes in cell viability. |
format | Online Article Text |
id | pubmed-9865979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98659792023-01-22 Identification of Nanoparticle Properties for Optimal Drug Delivery across a Physiological Cell Barrier Ross, Aisling M. Cahalane, Rachel M. Walsh, Darragh R. Grabrucker, Andreas M. Marcar, Lynnette Mulvihill, John J. E. Pharmaceutics Article Nanoparticles (NPs) represent an attractive strategy to overcome difficulties associated with the delivery of therapeutics. Knowing the optimal properties of NPs to address these issues could allow for improved in vivo responses. This work investigated NPs prepared from 5 materials of 3 sizes and 3 concentrations applied to a cell barrier model. The NPs permeability across a cell barrier and their effects on cell barrier integrity and cell viability were evaluated. The properties of these NPs, as determined in water (traditional) vs. media (realistic), were compared to cell responses. It was found that for all cellular activities, NP properties determined in media was the best predictor of the cell response. Notably, ZnO NPs caused significant alterations to cell viability across all 3 cell lines tested. Importantly, we report that the zeta potential of NPs correlates significantly with NP permeability and NP-induced changes in cell viability. NPs with physiological-based zeta potential of −12 mV result in good cell barrier penetration without considerable changes in cell viability. MDPI 2023-01-06 /pmc/articles/PMC9865979/ /pubmed/36678829 http://dx.doi.org/10.3390/pharmaceutics15010200 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ross, Aisling M. Cahalane, Rachel M. Walsh, Darragh R. Grabrucker, Andreas M. Marcar, Lynnette Mulvihill, John J. E. Identification of Nanoparticle Properties for Optimal Drug Delivery across a Physiological Cell Barrier |
title | Identification of Nanoparticle Properties for Optimal Drug Delivery across a Physiological Cell Barrier |
title_full | Identification of Nanoparticle Properties for Optimal Drug Delivery across a Physiological Cell Barrier |
title_fullStr | Identification of Nanoparticle Properties for Optimal Drug Delivery across a Physiological Cell Barrier |
title_full_unstemmed | Identification of Nanoparticle Properties for Optimal Drug Delivery across a Physiological Cell Barrier |
title_short | Identification of Nanoparticle Properties for Optimal Drug Delivery across a Physiological Cell Barrier |
title_sort | identification of nanoparticle properties for optimal drug delivery across a physiological cell barrier |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9865979/ https://www.ncbi.nlm.nih.gov/pubmed/36678829 http://dx.doi.org/10.3390/pharmaceutics15010200 |
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