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Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins

This study assessed the efficacy of five denture cleansers on the microbial adherence and surface topography of conventional and CAD/CAM denture base resins. Acrylic resin discs were fabricated using conventional, milling, and 3D printing methods (N = 180). The discs were contaminated with dual spec...

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Autores principales: Alfouzan, Afnan F., Tuwaym, Malath, Aldaghri, Ebtihal N., Alojaymi, Tagreed, Alotiabi, Hadeel Minife, Taweel, Sara M. Al, Al-Otaibi, Hanan N., Ali, Rizwan, Alshehri, Huda, Labban, Nawaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866049/
https://www.ncbi.nlm.nih.gov/pubmed/36679340
http://dx.doi.org/10.3390/polym15020460
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author Alfouzan, Afnan F.
Tuwaym, Malath
Aldaghri, Ebtihal N.
Alojaymi, Tagreed
Alotiabi, Hadeel Minife
Taweel, Sara M. Al
Al-Otaibi, Hanan N.
Ali, Rizwan
Alshehri, Huda
Labban, Nawaf
author_facet Alfouzan, Afnan F.
Tuwaym, Malath
Aldaghri, Ebtihal N.
Alojaymi, Tagreed
Alotiabi, Hadeel Minife
Taweel, Sara M. Al
Al-Otaibi, Hanan N.
Ali, Rizwan
Alshehri, Huda
Labban, Nawaf
author_sort Alfouzan, Afnan F.
collection PubMed
description This study assessed the efficacy of five denture cleansers on the microbial adherence and surface topography of conventional and CAD/CAM denture base resins. Acrylic resin discs were fabricated using conventional, milling, and 3D printing methods (N = 180). The discs were contaminated with dual species of Candida albicans and Streptococcus mutans biofilm for 72 h and then disinfected with either of the denture cleansers (Fittydent cleansing tablets, 2% Chlorhexidine gluconate, 0.2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite (n = 10). Distilled water served as the control group. The colony-forming units of the microorganisms were calculated, followed by post-treatment surface roughness. Data were statistically analyzed using one-way ANOVA, paired t-test, and post hoc Tukey HSD test (α = 0.05). Among the denture cleansers, 2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite had the best cleansing effect on the resin discs and demonstrated zero growth of colonies for both the species. Comparing the material groups, the 3D-processed discs showed higher colony-forming units followed by the conventional and CAD/CAM milled group. The highest surface roughness was demonstrated by the 3D-printed discs (0.690 ± 0.08 μm), followed by the conventional (0.493 ± 0.11 μm) and the milled groups (0.301 ± 0.08 μm). The tested chemical denture cleansers affected the Candida albicans and Streptococcus mutans adhesion compared to control discs immersed in distilled water. The clinician may recommend to their patient to use 2% chlorhexidine gluconate for the disinfection of CAD/CAM PMMA denture base materials.
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spelling pubmed-98660492023-01-22 Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins Alfouzan, Afnan F. Tuwaym, Malath Aldaghri, Ebtihal N. Alojaymi, Tagreed Alotiabi, Hadeel Minife Taweel, Sara M. Al Al-Otaibi, Hanan N. Ali, Rizwan Alshehri, Huda Labban, Nawaf Polymers (Basel) Article This study assessed the efficacy of five denture cleansers on the microbial adherence and surface topography of conventional and CAD/CAM denture base resins. Acrylic resin discs were fabricated using conventional, milling, and 3D printing methods (N = 180). The discs were contaminated with dual species of Candida albicans and Streptococcus mutans biofilm for 72 h and then disinfected with either of the denture cleansers (Fittydent cleansing tablets, 2% Chlorhexidine gluconate, 0.2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite (n = 10). Distilled water served as the control group. The colony-forming units of the microorganisms were calculated, followed by post-treatment surface roughness. Data were statistically analyzed using one-way ANOVA, paired t-test, and post hoc Tukey HSD test (α = 0.05). Among the denture cleansers, 2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite had the best cleansing effect on the resin discs and demonstrated zero growth of colonies for both the species. Comparing the material groups, the 3D-processed discs showed higher colony-forming units followed by the conventional and CAD/CAM milled group. The highest surface roughness was demonstrated by the 3D-printed discs (0.690 ± 0.08 μm), followed by the conventional (0.493 ± 0.11 μm) and the milled groups (0.301 ± 0.08 μm). The tested chemical denture cleansers affected the Candida albicans and Streptococcus mutans adhesion compared to control discs immersed in distilled water. The clinician may recommend to their patient to use 2% chlorhexidine gluconate for the disinfection of CAD/CAM PMMA denture base materials. MDPI 2023-01-15 /pmc/articles/PMC9866049/ /pubmed/36679340 http://dx.doi.org/10.3390/polym15020460 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alfouzan, Afnan F.
Tuwaym, Malath
Aldaghri, Ebtihal N.
Alojaymi, Tagreed
Alotiabi, Hadeel Minife
Taweel, Sara M. Al
Al-Otaibi, Hanan N.
Ali, Rizwan
Alshehri, Huda
Labban, Nawaf
Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins
title Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins
title_full Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins
title_fullStr Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins
title_full_unstemmed Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins
title_short Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins
title_sort efficacy of denture cleansers on microbial adherence and surface topography of conventional and cad/cam-processed denture base resins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866049/
https://www.ncbi.nlm.nih.gov/pubmed/36679340
http://dx.doi.org/10.3390/polym15020460
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