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Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray

The amaranthine scale of the COVID-19 pandemic and unpredictable disease severity is of grave concern. Serological diagnostic aids are an excellent choice for clinicians for rapid and easy prognosis of the disease. To this end, we studied the humoral immune response to SARS-CoV-2 infection to map im...

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Autores principales: Acharjee, Arup, Ray, Arka, Salkar, Akanksha, Bihani, Surbhi, Tuckley, Chaitanya, Shastri, Jayanthi, Agrawal, Sachee, Duttagupta, Siddhartha, Srivastava, Sanjeeva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866125/
https://www.ncbi.nlm.nih.gov/pubmed/36680289
http://dx.doi.org/10.3390/v15010248
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author Acharjee, Arup
Ray, Arka
Salkar, Akanksha
Bihani, Surbhi
Tuckley, Chaitanya
Shastri, Jayanthi
Agrawal, Sachee
Duttagupta, Siddhartha
Srivastava, Sanjeeva
author_facet Acharjee, Arup
Ray, Arka
Salkar, Akanksha
Bihani, Surbhi
Tuckley, Chaitanya
Shastri, Jayanthi
Agrawal, Sachee
Duttagupta, Siddhartha
Srivastava, Sanjeeva
author_sort Acharjee, Arup
collection PubMed
description The amaranthine scale of the COVID-19 pandemic and unpredictable disease severity is of grave concern. Serological diagnostic aids are an excellent choice for clinicians for rapid and easy prognosis of the disease. To this end, we studied the humoral immune response to SARS-CoV-2 infection to map immunogenic regions in the SARS-CoV-2 proteome at amino acid resolution using a high-density SARS-CoV-2 proteome peptide microarray. The microarray has 4932 overlapping peptides printed in duplicates spanning the entire SARS-CoV-2 proteome. We found 204 and 676 immunogenic peptides against IgA and IgG, corresponding to 137 and 412 IgA and IgG epitopes, respectively. Of these, 6 and 307 epitopes could discriminate between disease severity. The emergence of variants has added to the complexity of the disease. Using the mutation panel available, we could detect 5 and 10 immunogenic peptides against IgA and IgG with mutations belonging to SAR-CoV-2 variants. The study revealed severity-based epitopes that could be presented as potential prognostic serological markers. Further, the mutant epitope immunogenicity could indicate the putative use of these markers for diagnosing variants responsible for the infection.
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spelling pubmed-98661252023-01-22 Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray Acharjee, Arup Ray, Arka Salkar, Akanksha Bihani, Surbhi Tuckley, Chaitanya Shastri, Jayanthi Agrawal, Sachee Duttagupta, Siddhartha Srivastava, Sanjeeva Viruses Article The amaranthine scale of the COVID-19 pandemic and unpredictable disease severity is of grave concern. Serological diagnostic aids are an excellent choice for clinicians for rapid and easy prognosis of the disease. To this end, we studied the humoral immune response to SARS-CoV-2 infection to map immunogenic regions in the SARS-CoV-2 proteome at amino acid resolution using a high-density SARS-CoV-2 proteome peptide microarray. The microarray has 4932 overlapping peptides printed in duplicates spanning the entire SARS-CoV-2 proteome. We found 204 and 676 immunogenic peptides against IgA and IgG, corresponding to 137 and 412 IgA and IgG epitopes, respectively. Of these, 6 and 307 epitopes could discriminate between disease severity. The emergence of variants has added to the complexity of the disease. Using the mutation panel available, we could detect 5 and 10 immunogenic peptides against IgA and IgG with mutations belonging to SAR-CoV-2 variants. The study revealed severity-based epitopes that could be presented as potential prognostic serological markers. Further, the mutant epitope immunogenicity could indicate the putative use of these markers for diagnosing variants responsible for the infection. MDPI 2023-01-15 /pmc/articles/PMC9866125/ /pubmed/36680289 http://dx.doi.org/10.3390/v15010248 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Acharjee, Arup
Ray, Arka
Salkar, Akanksha
Bihani, Surbhi
Tuckley, Chaitanya
Shastri, Jayanthi
Agrawal, Sachee
Duttagupta, Siddhartha
Srivastava, Sanjeeva
Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray
title Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray
title_full Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray
title_fullStr Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray
title_full_unstemmed Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray
title_short Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray
title_sort humoral immune response profile of covid-19 reveals severity and variant-specific epitopes: lessons from sars-cov-2 peptide microarray
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866125/
https://www.ncbi.nlm.nih.gov/pubmed/36680289
http://dx.doi.org/10.3390/v15010248
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