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Characterization and Chemical Synthesis of Cm39 (α-KTx 4.8): A Scorpion Toxin That Inhibits Voltage-Gated K(+) Channel K(V)1.2 and Small- and Intermediate-Conductance Ca(2+)-Activated K(+) Channels K(Ca)2.2 and K(Ca)3.1

A novel peptide, Cm39, was identified in the venom of the scorpion Centruroides margaritatus. Its primary structure was determined. It consists of 37 amino acid residues with a MW of 3980.2 Da. The full chemical synthesis and proper folding of Cm39 was obtained. Based on amino acid sequence alignmen...

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Autores principales: Naseem, Muhammad Umair, Gurrola-Briones, Georgina, Romero-Imbachi, Margarita R., Borrego, Jesus, Carcamo-Noriega, Edson, Beltrán-Vidal, José, Zamudio, Fernando Z., Shakeel, Kashmala, Possani, Lourival Domingos, Panyi, Gyorgy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866218/
https://www.ncbi.nlm.nih.gov/pubmed/36668861
http://dx.doi.org/10.3390/toxins15010041
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author Naseem, Muhammad Umair
Gurrola-Briones, Georgina
Romero-Imbachi, Margarita R.
Borrego, Jesus
Carcamo-Noriega, Edson
Beltrán-Vidal, José
Zamudio, Fernando Z.
Shakeel, Kashmala
Possani, Lourival Domingos
Panyi, Gyorgy
author_facet Naseem, Muhammad Umair
Gurrola-Briones, Georgina
Romero-Imbachi, Margarita R.
Borrego, Jesus
Carcamo-Noriega, Edson
Beltrán-Vidal, José
Zamudio, Fernando Z.
Shakeel, Kashmala
Possani, Lourival Domingos
Panyi, Gyorgy
author_sort Naseem, Muhammad Umair
collection PubMed
description A novel peptide, Cm39, was identified in the venom of the scorpion Centruroides margaritatus. Its primary structure was determined. It consists of 37 amino acid residues with a MW of 3980.2 Da. The full chemical synthesis and proper folding of Cm39 was obtained. Based on amino acid sequence alignment with different K(+) channel inhibitor scorpion toxin (KTx) families and phylogenetic analysis, Cm39 belongs to the α-KTx 4 family and was registered with the systematic number of α-KTx 4.8. Synthetic Cm39 inhibits the voltage-gated K(+) channel hK(V)1.2 with high affinity (K(d) = 65 nM). The conductance–voltage relationship of K(V)1.2 was not altered in the presence of Cm39, and the analysis of the toxin binding kinetics was consistent with a bimolecular interaction between the peptide and the channel; therefore, the pore blocking mechanism is proposed for the toxin–channel interaction. Cm39 also inhibits the Ca(2+)-activated K(Ca)2.2 and K(Ca)3.1 channels, with K(d) = 502 nM, and K(d) = 58 nM, respectively. However, the peptide does not inhibit hK(V)1.1, hK(V)1.3, hK(V)1.4, hK(V)1.5, hK(V)1.6, hK(V)11.1, mK(Ca)1.1 K(+) channels or the hNa(V)1.5 and hNa(V)1.4 Na(+) channels at 1 μM concentrations. Understanding the unusual selectivity profile of Cm39 motivates further experiments to reveal novel interactions with the vestibule of toxin-sensitive channels.
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spelling pubmed-98662182023-01-22 Characterization and Chemical Synthesis of Cm39 (α-KTx 4.8): A Scorpion Toxin That Inhibits Voltage-Gated K(+) Channel K(V)1.2 and Small- and Intermediate-Conductance Ca(2+)-Activated K(+) Channels K(Ca)2.2 and K(Ca)3.1 Naseem, Muhammad Umair Gurrola-Briones, Georgina Romero-Imbachi, Margarita R. Borrego, Jesus Carcamo-Noriega, Edson Beltrán-Vidal, José Zamudio, Fernando Z. Shakeel, Kashmala Possani, Lourival Domingos Panyi, Gyorgy Toxins (Basel) Article A novel peptide, Cm39, was identified in the venom of the scorpion Centruroides margaritatus. Its primary structure was determined. It consists of 37 amino acid residues with a MW of 3980.2 Da. The full chemical synthesis and proper folding of Cm39 was obtained. Based on amino acid sequence alignment with different K(+) channel inhibitor scorpion toxin (KTx) families and phylogenetic analysis, Cm39 belongs to the α-KTx 4 family and was registered with the systematic number of α-KTx 4.8. Synthetic Cm39 inhibits the voltage-gated K(+) channel hK(V)1.2 with high affinity (K(d) = 65 nM). The conductance–voltage relationship of K(V)1.2 was not altered in the presence of Cm39, and the analysis of the toxin binding kinetics was consistent with a bimolecular interaction between the peptide and the channel; therefore, the pore blocking mechanism is proposed for the toxin–channel interaction. Cm39 also inhibits the Ca(2+)-activated K(Ca)2.2 and K(Ca)3.1 channels, with K(d) = 502 nM, and K(d) = 58 nM, respectively. However, the peptide does not inhibit hK(V)1.1, hK(V)1.3, hK(V)1.4, hK(V)1.5, hK(V)1.6, hK(V)11.1, mK(Ca)1.1 K(+) channels or the hNa(V)1.5 and hNa(V)1.4 Na(+) channels at 1 μM concentrations. Understanding the unusual selectivity profile of Cm39 motivates further experiments to reveal novel interactions with the vestibule of toxin-sensitive channels. MDPI 2023-01-05 /pmc/articles/PMC9866218/ /pubmed/36668861 http://dx.doi.org/10.3390/toxins15010041 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Naseem, Muhammad Umair
Gurrola-Briones, Georgina
Romero-Imbachi, Margarita R.
Borrego, Jesus
Carcamo-Noriega, Edson
Beltrán-Vidal, José
Zamudio, Fernando Z.
Shakeel, Kashmala
Possani, Lourival Domingos
Panyi, Gyorgy
Characterization and Chemical Synthesis of Cm39 (α-KTx 4.8): A Scorpion Toxin That Inhibits Voltage-Gated K(+) Channel K(V)1.2 and Small- and Intermediate-Conductance Ca(2+)-Activated K(+) Channels K(Ca)2.2 and K(Ca)3.1
title Characterization and Chemical Synthesis of Cm39 (α-KTx 4.8): A Scorpion Toxin That Inhibits Voltage-Gated K(+) Channel K(V)1.2 and Small- and Intermediate-Conductance Ca(2+)-Activated K(+) Channels K(Ca)2.2 and K(Ca)3.1
title_full Characterization and Chemical Synthesis of Cm39 (α-KTx 4.8): A Scorpion Toxin That Inhibits Voltage-Gated K(+) Channel K(V)1.2 and Small- and Intermediate-Conductance Ca(2+)-Activated K(+) Channels K(Ca)2.2 and K(Ca)3.1
title_fullStr Characterization and Chemical Synthesis of Cm39 (α-KTx 4.8): A Scorpion Toxin That Inhibits Voltage-Gated K(+) Channel K(V)1.2 and Small- and Intermediate-Conductance Ca(2+)-Activated K(+) Channels K(Ca)2.2 and K(Ca)3.1
title_full_unstemmed Characterization and Chemical Synthesis of Cm39 (α-KTx 4.8): A Scorpion Toxin That Inhibits Voltage-Gated K(+) Channel K(V)1.2 and Small- and Intermediate-Conductance Ca(2+)-Activated K(+) Channels K(Ca)2.2 and K(Ca)3.1
title_short Characterization and Chemical Synthesis of Cm39 (α-KTx 4.8): A Scorpion Toxin That Inhibits Voltage-Gated K(+) Channel K(V)1.2 and Small- and Intermediate-Conductance Ca(2+)-Activated K(+) Channels K(Ca)2.2 and K(Ca)3.1
title_sort characterization and chemical synthesis of cm39 (α-ktx 4.8): a scorpion toxin that inhibits voltage-gated k(+) channel k(v)1.2 and small- and intermediate-conductance ca(2+)-activated k(+) channels k(ca)2.2 and k(ca)3.1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866218/
https://www.ncbi.nlm.nih.gov/pubmed/36668861
http://dx.doi.org/10.3390/toxins15010041
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