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Biologic Impact of Green Synthetized Magnetic Iron Oxide Nanoparticles on Two Different Lung Tumorigenic Monolayers and a 3D Normal Bronchial Model—EpiAirway(TM) Microtissue

The present study reports the successful synthesis of biocompatible magnetic iron oxide nanoparticles (MNPs) by an ecofriendly single step method, using two ethanolic extracts based on leaves of Camellia sinensis L. and Ocimum basilicum L. The effect of both green raw materials as reducing and cappi...

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Autores principales: Moacă, Elena-Alina, Watz, Claudia, Faur, Alexandra-Corina, Lazăr, Daniela, Socoliuc, Vlad, Păcurariu, Cornelia, Ianoș, Robert, Rus, Cristiana-Iulia, Minda, Daliana, Barbu-Tudoran, Lucian, Dehelean, Cristina Adriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866254/
https://www.ncbi.nlm.nih.gov/pubmed/36678632
http://dx.doi.org/10.3390/pharmaceutics15010002
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author Moacă, Elena-Alina
Watz, Claudia
Faur, Alexandra-Corina
Lazăr, Daniela
Socoliuc, Vlad
Păcurariu, Cornelia
Ianoș, Robert
Rus, Cristiana-Iulia
Minda, Daliana
Barbu-Tudoran, Lucian
Dehelean, Cristina Adriana
author_facet Moacă, Elena-Alina
Watz, Claudia
Faur, Alexandra-Corina
Lazăr, Daniela
Socoliuc, Vlad
Păcurariu, Cornelia
Ianoș, Robert
Rus, Cristiana-Iulia
Minda, Daliana
Barbu-Tudoran, Lucian
Dehelean, Cristina Adriana
author_sort Moacă, Elena-Alina
collection PubMed
description The present study reports the successful synthesis of biocompatible magnetic iron oxide nanoparticles (MNPs) by an ecofriendly single step method, using two ethanolic extracts based on leaves of Camellia sinensis L. and Ocimum basilicum L. The effect of both green raw materials as reducing and capping agents was taken into account for the development of MNPs, as well as the reaction synthesis temperature (25 °C and 80 °C). The biological effect of the MNPs obtained from Camellia sinensis L. ethanolic extract (Cs 25, Cs 80) was compared with that of the MNPs obtained from Ocimum basilicum L. ethanolic extract (Ob 25, Ob 80), by using two morphologically different lung cancer cell lines (A549 and NCI-H460); the results showed that the higher cell viability impairment was manifested by A549 cells after exposure to MNPs obtained from Ocimum basilicum L. ethanolic extract (Ob 25, Ob 80). Regarding the biosafety profile of the MNPs, it was shown that the EpiAirway(TM) models did not elicit important viability decrease or significant histopathological changes after treatment with none of the MNPs (Cs 25, Cs 80 and Ob 25, Ob 80), at concentrations up to 500 µg/mL.
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spelling pubmed-98662542023-01-22 Biologic Impact of Green Synthetized Magnetic Iron Oxide Nanoparticles on Two Different Lung Tumorigenic Monolayers and a 3D Normal Bronchial Model—EpiAirway(TM) Microtissue Moacă, Elena-Alina Watz, Claudia Faur, Alexandra-Corina Lazăr, Daniela Socoliuc, Vlad Păcurariu, Cornelia Ianoș, Robert Rus, Cristiana-Iulia Minda, Daliana Barbu-Tudoran, Lucian Dehelean, Cristina Adriana Pharmaceutics Article The present study reports the successful synthesis of biocompatible magnetic iron oxide nanoparticles (MNPs) by an ecofriendly single step method, using two ethanolic extracts based on leaves of Camellia sinensis L. and Ocimum basilicum L. The effect of both green raw materials as reducing and capping agents was taken into account for the development of MNPs, as well as the reaction synthesis temperature (25 °C and 80 °C). The biological effect of the MNPs obtained from Camellia sinensis L. ethanolic extract (Cs 25, Cs 80) was compared with that of the MNPs obtained from Ocimum basilicum L. ethanolic extract (Ob 25, Ob 80), by using two morphologically different lung cancer cell lines (A549 and NCI-H460); the results showed that the higher cell viability impairment was manifested by A549 cells after exposure to MNPs obtained from Ocimum basilicum L. ethanolic extract (Ob 25, Ob 80). Regarding the biosafety profile of the MNPs, it was shown that the EpiAirway(TM) models did not elicit important viability decrease or significant histopathological changes after treatment with none of the MNPs (Cs 25, Cs 80 and Ob 25, Ob 80), at concentrations up to 500 µg/mL. MDPI 2022-12-20 /pmc/articles/PMC9866254/ /pubmed/36678632 http://dx.doi.org/10.3390/pharmaceutics15010002 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moacă, Elena-Alina
Watz, Claudia
Faur, Alexandra-Corina
Lazăr, Daniela
Socoliuc, Vlad
Păcurariu, Cornelia
Ianoș, Robert
Rus, Cristiana-Iulia
Minda, Daliana
Barbu-Tudoran, Lucian
Dehelean, Cristina Adriana
Biologic Impact of Green Synthetized Magnetic Iron Oxide Nanoparticles on Two Different Lung Tumorigenic Monolayers and a 3D Normal Bronchial Model—EpiAirway(TM) Microtissue
title Biologic Impact of Green Synthetized Magnetic Iron Oxide Nanoparticles on Two Different Lung Tumorigenic Monolayers and a 3D Normal Bronchial Model—EpiAirway(TM) Microtissue
title_full Biologic Impact of Green Synthetized Magnetic Iron Oxide Nanoparticles on Two Different Lung Tumorigenic Monolayers and a 3D Normal Bronchial Model—EpiAirway(TM) Microtissue
title_fullStr Biologic Impact of Green Synthetized Magnetic Iron Oxide Nanoparticles on Two Different Lung Tumorigenic Monolayers and a 3D Normal Bronchial Model—EpiAirway(TM) Microtissue
title_full_unstemmed Biologic Impact of Green Synthetized Magnetic Iron Oxide Nanoparticles on Two Different Lung Tumorigenic Monolayers and a 3D Normal Bronchial Model—EpiAirway(TM) Microtissue
title_short Biologic Impact of Green Synthetized Magnetic Iron Oxide Nanoparticles on Two Different Lung Tumorigenic Monolayers and a 3D Normal Bronchial Model—EpiAirway(TM) Microtissue
title_sort biologic impact of green synthetized magnetic iron oxide nanoparticles on two different lung tumorigenic monolayers and a 3d normal bronchial model—epiairway(tm) microtissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866254/
https://www.ncbi.nlm.nih.gov/pubmed/36678632
http://dx.doi.org/10.3390/pharmaceutics15010002
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