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Sensibility and Specificity of the VitaPCR™ SARS-CoV-2 Assay for the Rapid Diagnosis of COVID-19 in Older Adults in the Emergency Department
(1) Background: During the COVID-19 pandemic, rapid and reliable diagnostic tools are needed for detecting SARS-CoV-2 infection in urgent cases at admission to the hospital. We aimed to assess the performances of the rapid molecular VitaPCR™ test (Menarini Diagnostics) in a sample of older adults ad...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866422/ https://www.ncbi.nlm.nih.gov/pubmed/36680229 http://dx.doi.org/10.3390/v15010189 |
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author | Piacenza, Francesco Cherubini, Antonio Galeazzi, Roberta Cardelli, Maurizio Giacconi, Robertina Pierpaoli, Elisa Marchegiani, Francesca Marcheselli, Fiorella Recchioni, Rina Casoli, Tiziana Farnocchia, Elisabetta Bartozzi, Beatrice Giorgetti, Belinda Stripoli, Pierpaolo Bonfigli, Anna Rita Fedecostante, Massimiliano Salvi, Fabio Pansoni, Adolfo Provinciali, Mauro Lattanzio, Fabrizia |
author_facet | Piacenza, Francesco Cherubini, Antonio Galeazzi, Roberta Cardelli, Maurizio Giacconi, Robertina Pierpaoli, Elisa Marchegiani, Francesca Marcheselli, Fiorella Recchioni, Rina Casoli, Tiziana Farnocchia, Elisabetta Bartozzi, Beatrice Giorgetti, Belinda Stripoli, Pierpaolo Bonfigli, Anna Rita Fedecostante, Massimiliano Salvi, Fabio Pansoni, Adolfo Provinciali, Mauro Lattanzio, Fabrizia |
author_sort | Piacenza, Francesco |
collection | PubMed |
description | (1) Background: During the COVID-19 pandemic, rapid and reliable diagnostic tools are needed for detecting SARS-CoV-2 infection in urgent cases at admission to the hospital. We aimed to assess the performances of the rapid molecular VitaPCR™ test (Menarini Diagnostics) in a sample of older adults admitted to the Emergency Department of two Italian hospitals (2) Methods: The comparison between the rapid VitaPCR™ and the RT-PCR was performed in 1695 samples. Two naso-pharyngeal swab samplings from each individual were obtained and processed using the VitaPCR™ and the RT-PCR for the detection of SARS-CoV-2 (3) Results: VitaPCR™ exhibited good precision (<3% CV) and an almost perfect overall agreement (Cohen’s K = 0.90) with the RT-PCR. The limit of detection of the VitaPCR™ was 4.1 copies/µL. Compared to the RT-PCR, the sensitivity, the specificity, and the positive and negative predictive values of VitaPCR™ were 83.4%, 99.9%, 99.2% and 98.3%, respectively (4) Conclusions: The VitaPCR™ showed similar sensitivity and specificity to other molecular-based rapid tests. This study suggests that the VitaPCR™ can allow the rapid management of patients within the Emergency Department. Nevertheless, it is advisable to obtain a negative result by a RT-PCR assay before admitting a patient to a regular ward. |
format | Online Article Text |
id | pubmed-9866422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98664222023-01-22 Sensibility and Specificity of the VitaPCR™ SARS-CoV-2 Assay for the Rapid Diagnosis of COVID-19 in Older Adults in the Emergency Department Piacenza, Francesco Cherubini, Antonio Galeazzi, Roberta Cardelli, Maurizio Giacconi, Robertina Pierpaoli, Elisa Marchegiani, Francesca Marcheselli, Fiorella Recchioni, Rina Casoli, Tiziana Farnocchia, Elisabetta Bartozzi, Beatrice Giorgetti, Belinda Stripoli, Pierpaolo Bonfigli, Anna Rita Fedecostante, Massimiliano Salvi, Fabio Pansoni, Adolfo Provinciali, Mauro Lattanzio, Fabrizia Viruses Article (1) Background: During the COVID-19 pandemic, rapid and reliable diagnostic tools are needed for detecting SARS-CoV-2 infection in urgent cases at admission to the hospital. We aimed to assess the performances of the rapid molecular VitaPCR™ test (Menarini Diagnostics) in a sample of older adults admitted to the Emergency Department of two Italian hospitals (2) Methods: The comparison between the rapid VitaPCR™ and the RT-PCR was performed in 1695 samples. Two naso-pharyngeal swab samplings from each individual were obtained and processed using the VitaPCR™ and the RT-PCR for the detection of SARS-CoV-2 (3) Results: VitaPCR™ exhibited good precision (<3% CV) and an almost perfect overall agreement (Cohen’s K = 0.90) with the RT-PCR. The limit of detection of the VitaPCR™ was 4.1 copies/µL. Compared to the RT-PCR, the sensitivity, the specificity, and the positive and negative predictive values of VitaPCR™ were 83.4%, 99.9%, 99.2% and 98.3%, respectively (4) Conclusions: The VitaPCR™ showed similar sensitivity and specificity to other molecular-based rapid tests. This study suggests that the VitaPCR™ can allow the rapid management of patients within the Emergency Department. Nevertheless, it is advisable to obtain a negative result by a RT-PCR assay before admitting a patient to a regular ward. MDPI 2023-01-09 /pmc/articles/PMC9866422/ /pubmed/36680229 http://dx.doi.org/10.3390/v15010189 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Piacenza, Francesco Cherubini, Antonio Galeazzi, Roberta Cardelli, Maurizio Giacconi, Robertina Pierpaoli, Elisa Marchegiani, Francesca Marcheselli, Fiorella Recchioni, Rina Casoli, Tiziana Farnocchia, Elisabetta Bartozzi, Beatrice Giorgetti, Belinda Stripoli, Pierpaolo Bonfigli, Anna Rita Fedecostante, Massimiliano Salvi, Fabio Pansoni, Adolfo Provinciali, Mauro Lattanzio, Fabrizia Sensibility and Specificity of the VitaPCR™ SARS-CoV-2 Assay for the Rapid Diagnosis of COVID-19 in Older Adults in the Emergency Department |
title | Sensibility and Specificity of the VitaPCR™ SARS-CoV-2 Assay for the Rapid Diagnosis of COVID-19 in Older Adults in the Emergency Department |
title_full | Sensibility and Specificity of the VitaPCR™ SARS-CoV-2 Assay for the Rapid Diagnosis of COVID-19 in Older Adults in the Emergency Department |
title_fullStr | Sensibility and Specificity of the VitaPCR™ SARS-CoV-2 Assay for the Rapid Diagnosis of COVID-19 in Older Adults in the Emergency Department |
title_full_unstemmed | Sensibility and Specificity of the VitaPCR™ SARS-CoV-2 Assay for the Rapid Diagnosis of COVID-19 in Older Adults in the Emergency Department |
title_short | Sensibility and Specificity of the VitaPCR™ SARS-CoV-2 Assay for the Rapid Diagnosis of COVID-19 in Older Adults in the Emergency Department |
title_sort | sensibility and specificity of the vitapcr™ sars-cov-2 assay for the rapid diagnosis of covid-19 in older adults in the emergency department |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866422/ https://www.ncbi.nlm.nih.gov/pubmed/36680229 http://dx.doi.org/10.3390/v15010189 |
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