Cargando…
Occurrence and Molecular Characterization of Potentially Pathogenic Vibrio spp. in Seafood Collected in Sicily
Seafood can vehiculate foodborne illnesses from water to humans. Climate changes, increasing water contamination and coastlines anthropization, favor the global spread of Vibrio spp. and the occurrence of antibiotic-resistant isolates. The aim of this study was to evaluate the spread of potentially...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866474/ https://www.ncbi.nlm.nih.gov/pubmed/36677345 http://dx.doi.org/10.3390/microorganisms11010053 |
_version_ | 1784876100737826816 |
---|---|
author | Castello, Annamaria Alio, Vincenzina Sciortino, Sonia Oliveri, Giuseppa Cardamone, Cinzia Butera, Gaspare Costa, Antonella |
author_facet | Castello, Annamaria Alio, Vincenzina Sciortino, Sonia Oliveri, Giuseppa Cardamone, Cinzia Butera, Gaspare Costa, Antonella |
author_sort | Castello, Annamaria |
collection | PubMed |
description | Seafood can vehiculate foodborne illnesses from water to humans. Climate changes, increasing water contamination and coastlines anthropization, favor the global spread of Vibrio spp. and the occurrence of antibiotic-resistant isolates. The aim of this study was to evaluate the spread of potentially pathogenic Vibrio spp. in fishery products collected in Sicily and to assess their antibiotic resistance. Bacteriological and molecular methods were applied to 603 seafood samples to detect V. parahaemolyticus, V. cholerae, V. vulnificus, and Vibrio alginolyticus in order to assess their pathogenicity and antimicrobial resistance. About 30% of bivalves and 20% of other fishery products were contaminated by Vibrio spp.; V. parahaemolyticus accounted for 43/165 isolates, 3 of which were carrying either tdh or trh; V. cholerae accounted for 12/165 isolates, all of them non-O1 non-O139 and none carrying virulence genes; and V. vulnificus accounted for 5/165 isolates. The highest rates of resistance were observed for ampicillin, but we also detected strains resistant to antibiotics currently included among the most efficient against Vibrio spp. In spite of their current low incidence, their rise might pose further issues in treating infections; hence, these results stress the need for a continuous monitoring of antimicrobial resistance among fishery products and an effective risk assessment. |
format | Online Article Text |
id | pubmed-9866474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98664742023-01-22 Occurrence and Molecular Characterization of Potentially Pathogenic Vibrio spp. in Seafood Collected in Sicily Castello, Annamaria Alio, Vincenzina Sciortino, Sonia Oliveri, Giuseppa Cardamone, Cinzia Butera, Gaspare Costa, Antonella Microorganisms Article Seafood can vehiculate foodborne illnesses from water to humans. Climate changes, increasing water contamination and coastlines anthropization, favor the global spread of Vibrio spp. and the occurrence of antibiotic-resistant isolates. The aim of this study was to evaluate the spread of potentially pathogenic Vibrio spp. in fishery products collected in Sicily and to assess their antibiotic resistance. Bacteriological and molecular methods were applied to 603 seafood samples to detect V. parahaemolyticus, V. cholerae, V. vulnificus, and Vibrio alginolyticus in order to assess their pathogenicity and antimicrobial resistance. About 30% of bivalves and 20% of other fishery products were contaminated by Vibrio spp.; V. parahaemolyticus accounted for 43/165 isolates, 3 of which were carrying either tdh or trh; V. cholerae accounted for 12/165 isolates, all of them non-O1 non-O139 and none carrying virulence genes; and V. vulnificus accounted for 5/165 isolates. The highest rates of resistance were observed for ampicillin, but we also detected strains resistant to antibiotics currently included among the most efficient against Vibrio spp. In spite of their current low incidence, their rise might pose further issues in treating infections; hence, these results stress the need for a continuous monitoring of antimicrobial resistance among fishery products and an effective risk assessment. MDPI 2022-12-23 /pmc/articles/PMC9866474/ /pubmed/36677345 http://dx.doi.org/10.3390/microorganisms11010053 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Castello, Annamaria Alio, Vincenzina Sciortino, Sonia Oliveri, Giuseppa Cardamone, Cinzia Butera, Gaspare Costa, Antonella Occurrence and Molecular Characterization of Potentially Pathogenic Vibrio spp. in Seafood Collected in Sicily |
title | Occurrence and Molecular Characterization of Potentially Pathogenic Vibrio spp. in Seafood Collected in Sicily |
title_full | Occurrence and Molecular Characterization of Potentially Pathogenic Vibrio spp. in Seafood Collected in Sicily |
title_fullStr | Occurrence and Molecular Characterization of Potentially Pathogenic Vibrio spp. in Seafood Collected in Sicily |
title_full_unstemmed | Occurrence and Molecular Characterization of Potentially Pathogenic Vibrio spp. in Seafood Collected in Sicily |
title_short | Occurrence and Molecular Characterization of Potentially Pathogenic Vibrio spp. in Seafood Collected in Sicily |
title_sort | occurrence and molecular characterization of potentially pathogenic vibrio spp. in seafood collected in sicily |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866474/ https://www.ncbi.nlm.nih.gov/pubmed/36677345 http://dx.doi.org/10.3390/microorganisms11010053 |
work_keys_str_mv | AT castelloannamaria occurrenceandmolecularcharacterizationofpotentiallypathogenicvibriosppinseafoodcollectedinsicily AT aliovincenzina occurrenceandmolecularcharacterizationofpotentiallypathogenicvibriosppinseafoodcollectedinsicily AT sciortinosonia occurrenceandmolecularcharacterizationofpotentiallypathogenicvibriosppinseafoodcollectedinsicily AT oliverigiuseppa occurrenceandmolecularcharacterizationofpotentiallypathogenicvibriosppinseafoodcollectedinsicily AT cardamonecinzia occurrenceandmolecularcharacterizationofpotentiallypathogenicvibriosppinseafoodcollectedinsicily AT buteragaspare occurrenceandmolecularcharacterizationofpotentiallypathogenicvibriosppinseafoodcollectedinsicily AT costaantonella occurrenceandmolecularcharacterizationofpotentiallypathogenicvibriosppinseafoodcollectedinsicily |