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Oral Bioavailability and Metabolism of Hydroxytyrosol from Food Supplements

Table olives and olive oils are the main dietary sources of hydroxytyrosol (HT), a natural antioxidant compound that has emerged as a potential aid in protection against cardiovascular risk. Bioavailability studies with olive oils showed that HT is bioavailable from its free form and from conjugated...

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Autores principales: Bender, Cecilia, Strassmann, Sarah, Golz, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866489/
https://www.ncbi.nlm.nih.gov/pubmed/36678196
http://dx.doi.org/10.3390/nu15020325
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author Bender, Cecilia
Strassmann, Sarah
Golz, Christian
author_facet Bender, Cecilia
Strassmann, Sarah
Golz, Christian
author_sort Bender, Cecilia
collection PubMed
description Table olives and olive oils are the main dietary sources of hydroxytyrosol (HT), a natural antioxidant compound that has emerged as a potential aid in protection against cardiovascular risk. Bioavailability studies with olive oils showed that HT is bioavailable from its free form and from conjugated forms such as oleuropein and its aglycone. Still, its low dietary intake, poor bioavailability, and high inter-individual variability after absorption through the gastrointestinal tract hamper its full benefits. In a randomized, controlled, blinded, cross-over study, we investigated the impact of HT metabolism and bioavailability by comparing two olive-derived watery supplements containing different doses of HT (30.58 and 61.48 mg of HT/dosage). Additionally, HT-fortified olive oil was used in the control group. To this aim, plasma and urine samples were evaluated in 12 healthy volunteers following the intake of a single dose of the supplements or fortified olive oil. Blood and urine samples were collected at baseline and at 0.5, 1, 1.5, 2, 4, and 12 h after intake. HT and its metabolites were analyzed using UHPLC-DAD-MS/MS. Pharmacokinetic results showed that dietary HT administered through the food supplements is bioavailable and bioavailability increases with the administered dose. After intake, homovanillic acid, HT-3-O-sulphate, and 3,4-dihydroxyphenylacetic acid are the main metabolites found both in plasma and urine. The maximum concentrations in plasma peaked 30 min after intake. As bioavailability of a compound is a fundamental prerequisite for its effect, these results promise a good potential of both food supplements for protection against oxidative stress and the consequent cardiovascular risk.
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spelling pubmed-98664892023-01-22 Oral Bioavailability and Metabolism of Hydroxytyrosol from Food Supplements Bender, Cecilia Strassmann, Sarah Golz, Christian Nutrients Article Table olives and olive oils are the main dietary sources of hydroxytyrosol (HT), a natural antioxidant compound that has emerged as a potential aid in protection against cardiovascular risk. Bioavailability studies with olive oils showed that HT is bioavailable from its free form and from conjugated forms such as oleuropein and its aglycone. Still, its low dietary intake, poor bioavailability, and high inter-individual variability after absorption through the gastrointestinal tract hamper its full benefits. In a randomized, controlled, blinded, cross-over study, we investigated the impact of HT metabolism and bioavailability by comparing two olive-derived watery supplements containing different doses of HT (30.58 and 61.48 mg of HT/dosage). Additionally, HT-fortified olive oil was used in the control group. To this aim, plasma and urine samples were evaluated in 12 healthy volunteers following the intake of a single dose of the supplements or fortified olive oil. Blood and urine samples were collected at baseline and at 0.5, 1, 1.5, 2, 4, and 12 h after intake. HT and its metabolites were analyzed using UHPLC-DAD-MS/MS. Pharmacokinetic results showed that dietary HT administered through the food supplements is bioavailable and bioavailability increases with the administered dose. After intake, homovanillic acid, HT-3-O-sulphate, and 3,4-dihydroxyphenylacetic acid are the main metabolites found both in plasma and urine. The maximum concentrations in plasma peaked 30 min after intake. As bioavailability of a compound is a fundamental prerequisite for its effect, these results promise a good potential of both food supplements for protection against oxidative stress and the consequent cardiovascular risk. MDPI 2023-01-09 /pmc/articles/PMC9866489/ /pubmed/36678196 http://dx.doi.org/10.3390/nu15020325 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bender, Cecilia
Strassmann, Sarah
Golz, Christian
Oral Bioavailability and Metabolism of Hydroxytyrosol from Food Supplements
title Oral Bioavailability and Metabolism of Hydroxytyrosol from Food Supplements
title_full Oral Bioavailability and Metabolism of Hydroxytyrosol from Food Supplements
title_fullStr Oral Bioavailability and Metabolism of Hydroxytyrosol from Food Supplements
title_full_unstemmed Oral Bioavailability and Metabolism of Hydroxytyrosol from Food Supplements
title_short Oral Bioavailability and Metabolism of Hydroxytyrosol from Food Supplements
title_sort oral bioavailability and metabolism of hydroxytyrosol from food supplements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866489/
https://www.ncbi.nlm.nih.gov/pubmed/36678196
http://dx.doi.org/10.3390/nu15020325
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