Cargando…

Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury

The main cause of erectile dysfunction (ED) is the damage in penile cavernous endothelial cells (EC). Murine primary ECs have a limited growth potential, and the easy availability of murine ECs will facilitate the study of cavernous endothelial dysfunction in rats. This study was performed to establ...

Descripción completa

Detalles Bibliográficos
Autores principales: Bak, Sang Hong, Kim, Jae Heon, Kim, Seung U., Lee, Dong-Seok, Song, Yun Seob, Lee, Hong J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866507/
https://www.ncbi.nlm.nih.gov/pubmed/36678621
http://dx.doi.org/10.3390/ph16010123
_version_ 1784876109220806656
author Bak, Sang Hong
Kim, Jae Heon
Kim, Seung U.
Lee, Dong-Seok
Song, Yun Seob
Lee, Hong J.
author_facet Bak, Sang Hong
Kim, Jae Heon
Kim, Seung U.
Lee, Dong-Seok
Song, Yun Seob
Lee, Hong J.
author_sort Bak, Sang Hong
collection PubMed
description The main cause of erectile dysfunction (ED) is the damage in penile cavernous endothelial cells (EC). Murine primary ECs have a limited growth potential, and the easy availability of murine ECs will facilitate the study of cavernous endothelial dysfunction in rats. This study was performed to establish immortalized rat penile cavernous ECs (rEC) and investigate how they could repair erectile dysfunction in rats with cavernous nerve injury (CNI). rEC was isolated enzymatically by collagenase digestion and were cultured. An amphotropic replication-incompetent retroviral vector encoding v-myc oncogene was used to transfect rEC for immortalization (vREC). Morphological and immunohistochemical properties of vREC were examined. Eight-week-old male Sprague-Dawley rats were divided into three groups of five rats each, including group 1 = sham operation, group 2 = bilateral CN injury, group 3 = vREC (1 × 10(6) cells) treatment after CNI. Erectile response was assessed at 2, 4 weeks after transplantation of vREC., Penile tissue were harvested at 4 weeks after transplantation and immune–histochemical examination was performed. vREC showed the expression of CD31, vWF, cell type-specific markers for EC by RT-PCR and flowcytometry. At 2, 4 weeks after transplantation, rats with CNI had significantly lower erectile function than control group (p < 0.05). The group transplanted with vREC showed higher erectile function than the group without vRECs (p < 0.05). vREC was established and repaired erectile dysfunction in rats with CNI. This cell line may be useful for studying mechanisms and drug screening of erectile dysfunction of rats.
format Online
Article
Text
id pubmed-9866507
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98665072023-01-22 Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury Bak, Sang Hong Kim, Jae Heon Kim, Seung U. Lee, Dong-Seok Song, Yun Seob Lee, Hong J. Pharmaceuticals (Basel) Article The main cause of erectile dysfunction (ED) is the damage in penile cavernous endothelial cells (EC). Murine primary ECs have a limited growth potential, and the easy availability of murine ECs will facilitate the study of cavernous endothelial dysfunction in rats. This study was performed to establish immortalized rat penile cavernous ECs (rEC) and investigate how they could repair erectile dysfunction in rats with cavernous nerve injury (CNI). rEC was isolated enzymatically by collagenase digestion and were cultured. An amphotropic replication-incompetent retroviral vector encoding v-myc oncogene was used to transfect rEC for immortalization (vREC). Morphological and immunohistochemical properties of vREC were examined. Eight-week-old male Sprague-Dawley rats were divided into three groups of five rats each, including group 1 = sham operation, group 2 = bilateral CN injury, group 3 = vREC (1 × 10(6) cells) treatment after CNI. Erectile response was assessed at 2, 4 weeks after transplantation of vREC., Penile tissue were harvested at 4 weeks after transplantation and immune–histochemical examination was performed. vREC showed the expression of CD31, vWF, cell type-specific markers for EC by RT-PCR and flowcytometry. At 2, 4 weeks after transplantation, rats with CNI had significantly lower erectile function than control group (p < 0.05). The group transplanted with vREC showed higher erectile function than the group without vRECs (p < 0.05). vREC was established and repaired erectile dysfunction in rats with CNI. This cell line may be useful for studying mechanisms and drug screening of erectile dysfunction of rats. MDPI 2023-01-13 /pmc/articles/PMC9866507/ /pubmed/36678621 http://dx.doi.org/10.3390/ph16010123 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bak, Sang Hong
Kim, Jae Heon
Kim, Seung U.
Lee, Dong-Seok
Song, Yun Seob
Lee, Hong J.
Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury
title Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury
title_full Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury
title_fullStr Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury
title_full_unstemmed Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury
title_short Established Immortalized Cavernous Endothelial Cells Improve Erectile Dysfunction in Rats with Cavernous Nerve Injury
title_sort established immortalized cavernous endothelial cells improve erectile dysfunction in rats with cavernous nerve injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866507/
https://www.ncbi.nlm.nih.gov/pubmed/36678621
http://dx.doi.org/10.3390/ph16010123
work_keys_str_mv AT baksanghong establishedimmortalizedcavernousendothelialcellsimproveerectiledysfunctioninratswithcavernousnerveinjury
AT kimjaeheon establishedimmortalizedcavernousendothelialcellsimproveerectiledysfunctioninratswithcavernousnerveinjury
AT kimseungu establishedimmortalizedcavernousendothelialcellsimproveerectiledysfunctioninratswithcavernousnerveinjury
AT leedongseok establishedimmortalizedcavernousendothelialcellsimproveerectiledysfunctioninratswithcavernousnerveinjury
AT songyunseob establishedimmortalizedcavernousendothelialcellsimproveerectiledysfunctioninratswithcavernousnerveinjury
AT leehongj establishedimmortalizedcavernousendothelialcellsimproveerectiledysfunctioninratswithcavernousnerveinjury