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Genetic Diversity and Expanded Host Range of J Paramyxovirus Detected in Wild Small Mammals in China

J paramyxovirus (JPV) is a rodent-borne Jeilongvirus isolated from moribund mice (Mus musculus) with hemorrhagic lung lesions trapped in the 1972 in northern Queensland, Australia. The JPV antibodies have been detected in wild mice, wild rats, pigs, and human populations in Australia. Here, by next-...

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Autores principales: Zhang, Yunfa, Zhang, Jingtao, Wang, Yuna, Tian, Feng, Zhang, Xiaolong, Wang, Gang, Li, Shuang, Ding, Heng, Hu, Zhenyu, Liu, Wei, Zhang, Xiaoai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866557/
https://www.ncbi.nlm.nih.gov/pubmed/36680089
http://dx.doi.org/10.3390/v15010049
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author Zhang, Yunfa
Zhang, Jingtao
Wang, Yuna
Tian, Feng
Zhang, Xiaolong
Wang, Gang
Li, Shuang
Ding, Heng
Hu, Zhenyu
Liu, Wei
Zhang, Xiaoai
author_facet Zhang, Yunfa
Zhang, Jingtao
Wang, Yuna
Tian, Feng
Zhang, Xiaolong
Wang, Gang
Li, Shuang
Ding, Heng
Hu, Zhenyu
Liu, Wei
Zhang, Xiaoai
author_sort Zhang, Yunfa
collection PubMed
description J paramyxovirus (JPV) is a rodent-borne Jeilongvirus isolated from moribund mice (Mus musculus) with hemorrhagic lung lesions trapped in the 1972 in northern Queensland, Australia. The JPV antibodies have been detected in wild mice, wild rats, pigs, and human populations in Australia. Here, by next-generation sequencing (NGS), we detected JPV from M. musculus in Shandong Province of China. Molecular detection of JPV was performed to survey to survey the infection among 66 species of wild small mammals collected from six eco-climate regions in China by applying JPV specific RT-PCR and sequencing. Altogether, 21 out of 3070 (0.68%) wild small mammals of four species were positive for JPV, including 5.26% (1/19) of Microtus fortis, 3.76% (17/452) of M. musculus, 1.67% (1/60) of Apodemus peninsulae, and 0.48% (2/421) of Apodemus agrarius, which captured three eco-climate regions of China (northeastern China, northern China, and Inner Mongolia-Xinjiang). Sequence analysis revealed the currently identified JPV was clustered with other 14 Jeilongvirus members, and shared 80.2% and 89.2% identity with Australia’s JPV partial RNA polymerase (L) and glycoprotein (G) genes, respectively. Phylogenetic analysis demonstrated the separation of three lineages of the current JPV sequences. Our results show three new hosts (A. agrarius, A. peninsulae, and M. fortis) for JPV, most of which were widely distributed in China, and highlight the potential zoonotic transmission of JPV in humans. The detection of JPV in wild small mammals in China broaden the viral diversity, geographical distribution, and reservoir types of JPV. Future studies should prioritize determining the epidemiological characteristics of JPV, so that potential risks can be mitigated.
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spelling pubmed-98665572023-01-22 Genetic Diversity and Expanded Host Range of J Paramyxovirus Detected in Wild Small Mammals in China Zhang, Yunfa Zhang, Jingtao Wang, Yuna Tian, Feng Zhang, Xiaolong Wang, Gang Li, Shuang Ding, Heng Hu, Zhenyu Liu, Wei Zhang, Xiaoai Viruses Article J paramyxovirus (JPV) is a rodent-borne Jeilongvirus isolated from moribund mice (Mus musculus) with hemorrhagic lung lesions trapped in the 1972 in northern Queensland, Australia. The JPV antibodies have been detected in wild mice, wild rats, pigs, and human populations in Australia. Here, by next-generation sequencing (NGS), we detected JPV from M. musculus in Shandong Province of China. Molecular detection of JPV was performed to survey to survey the infection among 66 species of wild small mammals collected from six eco-climate regions in China by applying JPV specific RT-PCR and sequencing. Altogether, 21 out of 3070 (0.68%) wild small mammals of four species were positive for JPV, including 5.26% (1/19) of Microtus fortis, 3.76% (17/452) of M. musculus, 1.67% (1/60) of Apodemus peninsulae, and 0.48% (2/421) of Apodemus agrarius, which captured three eco-climate regions of China (northeastern China, northern China, and Inner Mongolia-Xinjiang). Sequence analysis revealed the currently identified JPV was clustered with other 14 Jeilongvirus members, and shared 80.2% and 89.2% identity with Australia’s JPV partial RNA polymerase (L) and glycoprotein (G) genes, respectively. Phylogenetic analysis demonstrated the separation of three lineages of the current JPV sequences. Our results show three new hosts (A. agrarius, A. peninsulae, and M. fortis) for JPV, most of which were widely distributed in China, and highlight the potential zoonotic transmission of JPV in humans. The detection of JPV in wild small mammals in China broaden the viral diversity, geographical distribution, and reservoir types of JPV. Future studies should prioritize determining the epidemiological characteristics of JPV, so that potential risks can be mitigated. MDPI 2022-12-23 /pmc/articles/PMC9866557/ /pubmed/36680089 http://dx.doi.org/10.3390/v15010049 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Yunfa
Zhang, Jingtao
Wang, Yuna
Tian, Feng
Zhang, Xiaolong
Wang, Gang
Li, Shuang
Ding, Heng
Hu, Zhenyu
Liu, Wei
Zhang, Xiaoai
Genetic Diversity and Expanded Host Range of J Paramyxovirus Detected in Wild Small Mammals in China
title Genetic Diversity and Expanded Host Range of J Paramyxovirus Detected in Wild Small Mammals in China
title_full Genetic Diversity and Expanded Host Range of J Paramyxovirus Detected in Wild Small Mammals in China
title_fullStr Genetic Diversity and Expanded Host Range of J Paramyxovirus Detected in Wild Small Mammals in China
title_full_unstemmed Genetic Diversity and Expanded Host Range of J Paramyxovirus Detected in Wild Small Mammals in China
title_short Genetic Diversity and Expanded Host Range of J Paramyxovirus Detected in Wild Small Mammals in China
title_sort genetic diversity and expanded host range of j paramyxovirus detected in wild small mammals in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866557/
https://www.ncbi.nlm.nih.gov/pubmed/36680089
http://dx.doi.org/10.3390/v15010049
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