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Identification of YABBY Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera

The plant-specific transcription factor family YABBY plays important roles in plant responses to biotic and abiotic stresses. Although the function of YABBY has been identified in many species, systematic analysis in lotus (Nelumbo nucifera) is still relatively lacking. The present study aimed to ch...

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Autores principales: Zhao, Shuping, Zhang, Yao, Tan, Mengying, Jiao, Jiao, Zhang, Chuyan, Wu, Peng, Feng, Kai, Li, Liangjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866709/
https://www.ncbi.nlm.nih.gov/pubmed/36679092
http://dx.doi.org/10.3390/plants12020380
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author Zhao, Shuping
Zhang, Yao
Tan, Mengying
Jiao, Jiao
Zhang, Chuyan
Wu, Peng
Feng, Kai
Li, Liangjun
author_facet Zhao, Shuping
Zhang, Yao
Tan, Mengying
Jiao, Jiao
Zhang, Chuyan
Wu, Peng
Feng, Kai
Li, Liangjun
author_sort Zhao, Shuping
collection PubMed
description The plant-specific transcription factor family YABBY plays important roles in plant responses to biotic and abiotic stresses. Although the function of YABBY has been identified in many species, systematic analysis in lotus (Nelumbo nucifera) is still relatively lacking. The present study aimed to characterize all of the YABBY genes in lotus and obtain better insights into NnYABBYs in response to salt stress by depending on ABA signaling. Here, we identified nine YABBY genes by searching the whole lotus genome based on the conserved YABBY domain. Further analysis showed that these members were distributed on six different chromosomes and named from YABBY1 to YABBY9, which were divided into five subgroups, including YAB1, YAB2, YAB5, INO, and CRC. The analysis of cis-elements in promotors revealed that NnYABBYs could be involved in plant hormone signaling and plant responses to abiotic stresses. Quantitative real-time PCR (qRT-PCR) showed that NnYABBYs could be up-regulated or down-regulated by ABA, fluridone, and salt treatment. Subcellular localization indicated that NnYABBY4, NnYABBY5, and NnYABBY6 were mainly localized in the cell membrane and cytoplasm. In addition, the intrinsic trans-activity of NnYABBY was tested by a Y2H assay, which revealed that NnYABBY4, NnYABBY5, and NnYABBY6 are deprived of such a property. This study provided a theoretical basis and reference for the functional research of YABBY for the molecular breeding of lotus.
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spelling pubmed-98667092023-01-22 Identification of YABBY Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera Zhao, Shuping Zhang, Yao Tan, Mengying Jiao, Jiao Zhang, Chuyan Wu, Peng Feng, Kai Li, Liangjun Plants (Basel) Article The plant-specific transcription factor family YABBY plays important roles in plant responses to biotic and abiotic stresses. Although the function of YABBY has been identified in many species, systematic analysis in lotus (Nelumbo nucifera) is still relatively lacking. The present study aimed to characterize all of the YABBY genes in lotus and obtain better insights into NnYABBYs in response to salt stress by depending on ABA signaling. Here, we identified nine YABBY genes by searching the whole lotus genome based on the conserved YABBY domain. Further analysis showed that these members were distributed on six different chromosomes and named from YABBY1 to YABBY9, which were divided into five subgroups, including YAB1, YAB2, YAB5, INO, and CRC. The analysis of cis-elements in promotors revealed that NnYABBYs could be involved in plant hormone signaling and plant responses to abiotic stresses. Quantitative real-time PCR (qRT-PCR) showed that NnYABBYs could be up-regulated or down-regulated by ABA, fluridone, and salt treatment. Subcellular localization indicated that NnYABBY4, NnYABBY5, and NnYABBY6 were mainly localized in the cell membrane and cytoplasm. In addition, the intrinsic trans-activity of NnYABBY was tested by a Y2H assay, which revealed that NnYABBY4, NnYABBY5, and NnYABBY6 are deprived of such a property. This study provided a theoretical basis and reference for the functional research of YABBY for the molecular breeding of lotus. MDPI 2023-01-13 /pmc/articles/PMC9866709/ /pubmed/36679092 http://dx.doi.org/10.3390/plants12020380 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Shuping
Zhang, Yao
Tan, Mengying
Jiao, Jiao
Zhang, Chuyan
Wu, Peng
Feng, Kai
Li, Liangjun
Identification of YABBY Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title Identification of YABBY Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title_full Identification of YABBY Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title_fullStr Identification of YABBY Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title_full_unstemmed Identification of YABBY Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title_short Identification of YABBY Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title_sort identification of yabby transcription factors and their function in aba and salinity response in nelumbo nucifera
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866709/
https://www.ncbi.nlm.nih.gov/pubmed/36679092
http://dx.doi.org/10.3390/plants12020380
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