Comparative Insights into Four Major Legume Sprouts Efficacies for Diabetes Management and Its Complications: Untargeted versus Targeted NMR Biochemometrics Approach

Interest in the consumption of seed sprouts is gradually increasing as functional foods in the modern Western diet owing to their several nutritional and health benefits. The present study aims to investigate four major legume sprouts derived from faba bean (Vicia faba L.), lentil (Lens esculenta L.), chickpea (Cicer arietinum L.), and fenugreek (Trigonella foenum-greacum L.) for their antidiabetic activity and mitigation of associated complications, i.e., oxidative stress, liver dysfunction, and lipid metabolism, compared with glibenclamide. Biochemical results presented herein further showed that the four sprouts exhibited significant hypoglycemic effects (p < 0.05), with improvement in decreasing of blood glucose levels at different degrees and with faba bean sprout most active at 348% improvement, compared to 364.3% for glibenclamide. Further biochemometric analysis based on a comparison between targeted versus untargeted partial least square (PLS) and regression analyses revealed that faba bean sprouts’ richness in flavonoids was a determinant key factor for such efficacy. In addition, correlation with previously investigated NMR fingerprinting aided in pinpointing other active agents, such as betaine and L-DOPA. Furthermore, the effect on serum liver enzymes, including alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase; oxidative stress markers; and lipid profiles showed significant improvement, especially in the case of faba bean sprout. The study revealed the potential health benefits of legume sprouts in the treatment of diabetes and its associated complications, as well as the potential role of biochemometrics in active agents’ identification in such a complex matrix to be considered for other functional foods investigation.