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Genotypic Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from an Egyptian University Hospital

Globally, Klebsiella pneumoniae (K. pneumoniae) has been identified as a serious source of infections. The objectives of our study were to investigate the prevalence of multidrug-resistant (MDR) K. pneumoniae in Tanta University Hospitals, Gharbia Governorate, Egypt; characterize their carbapenem re...

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Autores principales: Taha, Marwa S., Hagras, Maha M., Shalaby, Marwa M., Zamzam, Yosra Abdelmonem, Elkolaly, Reham M., Abdelwahab, Marwa A., Maxwell, Sara Youssef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866858/
https://www.ncbi.nlm.nih.gov/pubmed/36678469
http://dx.doi.org/10.3390/pathogens12010121
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author Taha, Marwa S.
Hagras, Maha M.
Shalaby, Marwa M.
Zamzam, Yosra Abdelmonem
Elkolaly, Reham M.
Abdelwahab, Marwa A.
Maxwell, Sara Youssef
author_facet Taha, Marwa S.
Hagras, Maha M.
Shalaby, Marwa M.
Zamzam, Yosra Abdelmonem
Elkolaly, Reham M.
Abdelwahab, Marwa A.
Maxwell, Sara Youssef
author_sort Taha, Marwa S.
collection PubMed
description Globally, Klebsiella pneumoniae (K. pneumoniae) has been identified as a serious source of infections. The objectives of our study were to investigate the prevalence of multidrug-resistant (MDR) K. pneumoniae in Tanta University Hospitals, Gharbia Governorate, Egypt; characterize their carbapenem resistance profiles; and identify their different capsular serotypes. We identified and isolated 160 (32%) K. pneumoniae from 500 different clinical samples, performed antimicrobial susceptibility testing, and then used multiplex PCR to detect carbapenemase genes and capsular serotypes K1, K2, K3, K5, K20, K54, and K57. We detected phenotypic carbapenem resistance in 31.3% (50/160) of the isolates; however, molecular assays revealed that 38.75% (62/160) of isolates were carrying carbapenemase-encoding genes. Generally, bla(OXA-48) was the prevalent gene (15.5%), followed by bla(VIM) (15%), bla(IMP) (7.5%), bla(KPC) (4%), and bla(NDM) (3.8%). Bla(VIM) and bla(OXA-48) correlated with phenotypic resistance in 91.67% and 88% of the isolates that harbored them, respectively. Capsular typing showed that the most prevalent pathotype was K1 (30.6%), followed by K57 (24.2%), K54 (19.35%), K20 (9.67%), and K2 (6.45%). A critical risk to community health is posed by the high incidence of multidrug-resistant (MDR) virulent K. pneumoniae isolates from our hospital, and our study examines this pathogen’s public health and epidemiological risks.
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spelling pubmed-98668582023-01-22 Genotypic Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from an Egyptian University Hospital Taha, Marwa S. Hagras, Maha M. Shalaby, Marwa M. Zamzam, Yosra Abdelmonem Elkolaly, Reham M. Abdelwahab, Marwa A. Maxwell, Sara Youssef Pathogens Article Globally, Klebsiella pneumoniae (K. pneumoniae) has been identified as a serious source of infections. The objectives of our study were to investigate the prevalence of multidrug-resistant (MDR) K. pneumoniae in Tanta University Hospitals, Gharbia Governorate, Egypt; characterize their carbapenem resistance profiles; and identify their different capsular serotypes. We identified and isolated 160 (32%) K. pneumoniae from 500 different clinical samples, performed antimicrobial susceptibility testing, and then used multiplex PCR to detect carbapenemase genes and capsular serotypes K1, K2, K3, K5, K20, K54, and K57. We detected phenotypic carbapenem resistance in 31.3% (50/160) of the isolates; however, molecular assays revealed that 38.75% (62/160) of isolates were carrying carbapenemase-encoding genes. Generally, bla(OXA-48) was the prevalent gene (15.5%), followed by bla(VIM) (15%), bla(IMP) (7.5%), bla(KPC) (4%), and bla(NDM) (3.8%). Bla(VIM) and bla(OXA-48) correlated with phenotypic resistance in 91.67% and 88% of the isolates that harbored them, respectively. Capsular typing showed that the most prevalent pathotype was K1 (30.6%), followed by K57 (24.2%), K54 (19.35%), K20 (9.67%), and K2 (6.45%). A critical risk to community health is posed by the high incidence of multidrug-resistant (MDR) virulent K. pneumoniae isolates from our hospital, and our study examines this pathogen’s public health and epidemiological risks. MDPI 2023-01-11 /pmc/articles/PMC9866858/ /pubmed/36678469 http://dx.doi.org/10.3390/pathogens12010121 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Taha, Marwa S.
Hagras, Maha M.
Shalaby, Marwa M.
Zamzam, Yosra Abdelmonem
Elkolaly, Reham M.
Abdelwahab, Marwa A.
Maxwell, Sara Youssef
Genotypic Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from an Egyptian University Hospital
title Genotypic Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from an Egyptian University Hospital
title_full Genotypic Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from an Egyptian University Hospital
title_fullStr Genotypic Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from an Egyptian University Hospital
title_full_unstemmed Genotypic Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from an Egyptian University Hospital
title_short Genotypic Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated from an Egyptian University Hospital
title_sort genotypic characterization of carbapenem-resistant klebsiella pneumoniae isolated from an egyptian university hospital
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9866858/
https://www.ncbi.nlm.nih.gov/pubmed/36678469
http://dx.doi.org/10.3390/pathogens12010121
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