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Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos
Ligand of Numb-protein X 2 (LNX2) is an E3 ubiquitin ligase that is known to regulate Notch signaling by participating in NUMB protein degradation. Notch signaling is important for differentiation and proliferation in mammals, and plays a significant role in blastocyst formation during early embryon...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867088/ https://www.ncbi.nlm.nih.gov/pubmed/36674899 http://dx.doi.org/10.3390/ijms24021385 |
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author | Lee, Seung-Jae Kim, Jaehwan Han, Gwidong Hong, Seung-Pyo Kim, Dayeon Cho, Chunghee |
author_facet | Lee, Seung-Jae Kim, Jaehwan Han, Gwidong Hong, Seung-Pyo Kim, Dayeon Cho, Chunghee |
author_sort | Lee, Seung-Jae |
collection | PubMed |
description | Ligand of Numb-protein X 2 (LNX2) is an E3 ubiquitin ligase that is known to regulate Notch signaling by participating in NUMB protein degradation. Notch signaling is important for differentiation and proliferation in mammals, and plays a significant role in blastocyst formation during early embryonic development. In this study, we investigated Lnx2 in mouse preimplantation embryos. Expression analysis showed that Lnx2 is expressed in oocytes and preimplantation embryos. Lnx2-knockdown embryos normally progress to the morula stage, but the majority of them do not develop into normal blastocysts. Transcript analysis revealed that the expression levels of genes critical for cell lineage specification, including octamer-binding transcription factor 4 (Oct4), are increased in Lnx2 knockdown embryos. Furthermore, the expression levels of Notch and Hippo signaling-related genes are also increased by Lnx2 knockdown. Collectively, our results show that Lnx2 is important for blastocyst formation in mice, suggest that this may act via lineage specification of inner cell mass, and further show that Lnx2 may be involved in transcriptionally regulating various genes implicated in early embryonic development. |
format | Online Article Text |
id | pubmed-9867088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98670882023-01-22 Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos Lee, Seung-Jae Kim, Jaehwan Han, Gwidong Hong, Seung-Pyo Kim, Dayeon Cho, Chunghee Int J Mol Sci Brief Report Ligand of Numb-protein X 2 (LNX2) is an E3 ubiquitin ligase that is known to regulate Notch signaling by participating in NUMB protein degradation. Notch signaling is important for differentiation and proliferation in mammals, and plays a significant role in blastocyst formation during early embryonic development. In this study, we investigated Lnx2 in mouse preimplantation embryos. Expression analysis showed that Lnx2 is expressed in oocytes and preimplantation embryos. Lnx2-knockdown embryos normally progress to the morula stage, but the majority of them do not develop into normal blastocysts. Transcript analysis revealed that the expression levels of genes critical for cell lineage specification, including octamer-binding transcription factor 4 (Oct4), are increased in Lnx2 knockdown embryos. Furthermore, the expression levels of Notch and Hippo signaling-related genes are also increased by Lnx2 knockdown. Collectively, our results show that Lnx2 is important for blastocyst formation in mice, suggest that this may act via lineage specification of inner cell mass, and further show that Lnx2 may be involved in transcriptionally regulating various genes implicated in early embryonic development. MDPI 2023-01-10 /pmc/articles/PMC9867088/ /pubmed/36674899 http://dx.doi.org/10.3390/ijms24021385 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Lee, Seung-Jae Kim, Jaehwan Han, Gwidong Hong, Seung-Pyo Kim, Dayeon Cho, Chunghee Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos |
title | Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos |
title_full | Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos |
title_fullStr | Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos |
title_full_unstemmed | Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos |
title_short | Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos |
title_sort | impaired blastocyst formation in lnx2-knockdown mouse embryos |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867088/ https://www.ncbi.nlm.nih.gov/pubmed/36674899 http://dx.doi.org/10.3390/ijms24021385 |
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