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Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos

Ligand of Numb-protein X 2 (LNX2) is an E3 ubiquitin ligase that is known to regulate Notch signaling by participating in NUMB protein degradation. Notch signaling is important for differentiation and proliferation in mammals, and plays a significant role in blastocyst formation during early embryon...

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Autores principales: Lee, Seung-Jae, Kim, Jaehwan, Han, Gwidong, Hong, Seung-Pyo, Kim, Dayeon, Cho, Chunghee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867088/
https://www.ncbi.nlm.nih.gov/pubmed/36674899
http://dx.doi.org/10.3390/ijms24021385
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author Lee, Seung-Jae
Kim, Jaehwan
Han, Gwidong
Hong, Seung-Pyo
Kim, Dayeon
Cho, Chunghee
author_facet Lee, Seung-Jae
Kim, Jaehwan
Han, Gwidong
Hong, Seung-Pyo
Kim, Dayeon
Cho, Chunghee
author_sort Lee, Seung-Jae
collection PubMed
description Ligand of Numb-protein X 2 (LNX2) is an E3 ubiquitin ligase that is known to regulate Notch signaling by participating in NUMB protein degradation. Notch signaling is important for differentiation and proliferation in mammals, and plays a significant role in blastocyst formation during early embryonic development. In this study, we investigated Lnx2 in mouse preimplantation embryos. Expression analysis showed that Lnx2 is expressed in oocytes and preimplantation embryos. Lnx2-knockdown embryos normally progress to the morula stage, but the majority of them do not develop into normal blastocysts. Transcript analysis revealed that the expression levels of genes critical for cell lineage specification, including octamer-binding transcription factor 4 (Oct4), are increased in Lnx2 knockdown embryos. Furthermore, the expression levels of Notch and Hippo signaling-related genes are also increased by Lnx2 knockdown. Collectively, our results show that Lnx2 is important for blastocyst formation in mice, suggest that this may act via lineage specification of inner cell mass, and further show that Lnx2 may be involved in transcriptionally regulating various genes implicated in early embryonic development.
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spelling pubmed-98670882023-01-22 Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos Lee, Seung-Jae Kim, Jaehwan Han, Gwidong Hong, Seung-Pyo Kim, Dayeon Cho, Chunghee Int J Mol Sci Brief Report Ligand of Numb-protein X 2 (LNX2) is an E3 ubiquitin ligase that is known to regulate Notch signaling by participating in NUMB protein degradation. Notch signaling is important for differentiation and proliferation in mammals, and plays a significant role in blastocyst formation during early embryonic development. In this study, we investigated Lnx2 in mouse preimplantation embryos. Expression analysis showed that Lnx2 is expressed in oocytes and preimplantation embryos. Lnx2-knockdown embryos normally progress to the morula stage, but the majority of them do not develop into normal blastocysts. Transcript analysis revealed that the expression levels of genes critical for cell lineage specification, including octamer-binding transcription factor 4 (Oct4), are increased in Lnx2 knockdown embryos. Furthermore, the expression levels of Notch and Hippo signaling-related genes are also increased by Lnx2 knockdown. Collectively, our results show that Lnx2 is important for blastocyst formation in mice, suggest that this may act via lineage specification of inner cell mass, and further show that Lnx2 may be involved in transcriptionally regulating various genes implicated in early embryonic development. MDPI 2023-01-10 /pmc/articles/PMC9867088/ /pubmed/36674899 http://dx.doi.org/10.3390/ijms24021385 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Lee, Seung-Jae
Kim, Jaehwan
Han, Gwidong
Hong, Seung-Pyo
Kim, Dayeon
Cho, Chunghee
Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos
title Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos
title_full Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos
title_fullStr Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos
title_full_unstemmed Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos
title_short Impaired Blastocyst Formation in Lnx2-Knockdown Mouse Embryos
title_sort impaired blastocyst formation in lnx2-knockdown mouse embryos
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867088/
https://www.ncbi.nlm.nih.gov/pubmed/36674899
http://dx.doi.org/10.3390/ijms24021385
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