Cargando…

Fungal Pathogens Associated with Crown and Root Rot in Wheat-Growing Areas of Northern Kyrgyzstan

Fungal species associated with crown and root rot diseases in wheat have been extensively studied in many parts of the world. However, no reports on the relative importance and distribution of pathogens associated with wheat crown and root rot in Kyrgyzstan have been published. Hence, fungal species...

Descripción completa

Detalles Bibliográficos
Autores principales: Özer, Göksel, Erper, İsmail, Yıldız, Şenol, Bozoğlu, Tuğba, Zholdoshbekova, Sezim, Alkan, Mehtap, Tekin, Fatih, Uulu, Tair Esenali, İmren, Mustafa, Dababat, Abdelfattah A., Derviş, Sibel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867107/
https://www.ncbi.nlm.nih.gov/pubmed/36675945
http://dx.doi.org/10.3390/jof9010124
_version_ 1784876260359405568
author Özer, Göksel
Erper, İsmail
Yıldız, Şenol
Bozoğlu, Tuğba
Zholdoshbekova, Sezim
Alkan, Mehtap
Tekin, Fatih
Uulu, Tair Esenali
İmren, Mustafa
Dababat, Abdelfattah A.
Derviş, Sibel
author_facet Özer, Göksel
Erper, İsmail
Yıldız, Şenol
Bozoğlu, Tuğba
Zholdoshbekova, Sezim
Alkan, Mehtap
Tekin, Fatih
Uulu, Tair Esenali
İmren, Mustafa
Dababat, Abdelfattah A.
Derviş, Sibel
author_sort Özer, Göksel
collection PubMed
description Fungal species associated with crown and root rot diseases in wheat have been extensively studied in many parts of the world. However, no reports on the relative importance and distribution of pathogens associated with wheat crown and root rot in Kyrgyzstan have been published. Hence, fungal species associated with wheat crown/root rot were surveyed in three main wheat production regions in northern Kyrgyzstan. Fungal species were isolated on 1/5 strength potato-dextrose agar amended with streptomycin (0.1 g/L) and chloramphenicol (0.05 g/L). A total of 598 fungal isolates from symptomatic tissues were identified using morphological features of the cultures and conidia, as well as sequence analysis of the nuclear ribosomal internal transcribed spacer (ITS) region, the translation elongation factor 1α (TEF1), and the RNA polymerase II beta subunit (RPB2) genes. The percentage of fields from which each fungus was isolated and their relative percentage isolation levels were determined. Bipolaris sorokiniana, the causal agent of common root rot, was the most prevalent pathogenic species isolated, being isolated from 86.67% of the fields surveyed at a frequency of isolation of 40.64%. Fusarium spp. accounted for 53.01% of all isolates and consisted of 12 different species. The most common Fusarium species identified was Fusarium acuminatum, which was isolated from 70% of the sites surveyed with an isolation frequency of 21.57%, followed by Fusarium culmorum, Fusarium nygamai, Fusarium oxysporum, and Fusarium equiseti, all of which had a field incidence of more than 23%. Inoculation tests with 44 isolates representing 17 species on the susceptible Triticum aestivum cv. Seri 82 revealed that Fusarium pseudograminearum and F. culmorum isolates were equally the most virulent pathogens. The widespread distribution of moderately virulent B. sorokiniana appears to be a serious threat to wheat culture, limiting yield and quality. With the exception of F. culmorum, the remaining Fusarium species did not pose a significant threat to wheat production in the surveyed areas because common species, such as F. acuminatum, F. nygamai, F. oxysporum, and F. equiseti, were non-pathogenic but infrequent species, such as Fusarium redolens, Fusarium algeriense, and F. pseudograminearum, were highly or moderately virulent. Curvularia inaequalis, which was found in three different fields, was mildly virulent. The remaining Fusarium species, Fusarium solani, Fusarium proliferatum, Fusarium burgessii, and Fusarium tricinctum, as well as Microdochium bolleyi, Microdochium nivale, and Macrophomina phaseolina, were non-pathogenic and considered to be secondary colonizers. The implications of these findings are discussed.
format Online
Article
Text
id pubmed-9867107
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98671072023-01-22 Fungal Pathogens Associated with Crown and Root Rot in Wheat-Growing Areas of Northern Kyrgyzstan Özer, Göksel Erper, İsmail Yıldız, Şenol Bozoğlu, Tuğba Zholdoshbekova, Sezim Alkan, Mehtap Tekin, Fatih Uulu, Tair Esenali İmren, Mustafa Dababat, Abdelfattah A. Derviş, Sibel J Fungi (Basel) Article Fungal species associated with crown and root rot diseases in wheat have been extensively studied in many parts of the world. However, no reports on the relative importance and distribution of pathogens associated with wheat crown and root rot in Kyrgyzstan have been published. Hence, fungal species associated with wheat crown/root rot were surveyed in three main wheat production regions in northern Kyrgyzstan. Fungal species were isolated on 1/5 strength potato-dextrose agar amended with streptomycin (0.1 g/L) and chloramphenicol (0.05 g/L). A total of 598 fungal isolates from symptomatic tissues were identified using morphological features of the cultures and conidia, as well as sequence analysis of the nuclear ribosomal internal transcribed spacer (ITS) region, the translation elongation factor 1α (TEF1), and the RNA polymerase II beta subunit (RPB2) genes. The percentage of fields from which each fungus was isolated and their relative percentage isolation levels were determined. Bipolaris sorokiniana, the causal agent of common root rot, was the most prevalent pathogenic species isolated, being isolated from 86.67% of the fields surveyed at a frequency of isolation of 40.64%. Fusarium spp. accounted for 53.01% of all isolates and consisted of 12 different species. The most common Fusarium species identified was Fusarium acuminatum, which was isolated from 70% of the sites surveyed with an isolation frequency of 21.57%, followed by Fusarium culmorum, Fusarium nygamai, Fusarium oxysporum, and Fusarium equiseti, all of which had a field incidence of more than 23%. Inoculation tests with 44 isolates representing 17 species on the susceptible Triticum aestivum cv. Seri 82 revealed that Fusarium pseudograminearum and F. culmorum isolates were equally the most virulent pathogens. The widespread distribution of moderately virulent B. sorokiniana appears to be a serious threat to wheat culture, limiting yield and quality. With the exception of F. culmorum, the remaining Fusarium species did not pose a significant threat to wheat production in the surveyed areas because common species, such as F. acuminatum, F. nygamai, F. oxysporum, and F. equiseti, were non-pathogenic but infrequent species, such as Fusarium redolens, Fusarium algeriense, and F. pseudograminearum, were highly or moderately virulent. Curvularia inaequalis, which was found in three different fields, was mildly virulent. The remaining Fusarium species, Fusarium solani, Fusarium proliferatum, Fusarium burgessii, and Fusarium tricinctum, as well as Microdochium bolleyi, Microdochium nivale, and Macrophomina phaseolina, were non-pathogenic and considered to be secondary colonizers. The implications of these findings are discussed. MDPI 2023-01-16 /pmc/articles/PMC9867107/ /pubmed/36675945 http://dx.doi.org/10.3390/jof9010124 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Özer, Göksel
Erper, İsmail
Yıldız, Şenol
Bozoğlu, Tuğba
Zholdoshbekova, Sezim
Alkan, Mehtap
Tekin, Fatih
Uulu, Tair Esenali
İmren, Mustafa
Dababat, Abdelfattah A.
Derviş, Sibel
Fungal Pathogens Associated with Crown and Root Rot in Wheat-Growing Areas of Northern Kyrgyzstan
title Fungal Pathogens Associated with Crown and Root Rot in Wheat-Growing Areas of Northern Kyrgyzstan
title_full Fungal Pathogens Associated with Crown and Root Rot in Wheat-Growing Areas of Northern Kyrgyzstan
title_fullStr Fungal Pathogens Associated with Crown and Root Rot in Wheat-Growing Areas of Northern Kyrgyzstan
title_full_unstemmed Fungal Pathogens Associated with Crown and Root Rot in Wheat-Growing Areas of Northern Kyrgyzstan
title_short Fungal Pathogens Associated with Crown and Root Rot in Wheat-Growing Areas of Northern Kyrgyzstan
title_sort fungal pathogens associated with crown and root rot in wheat-growing areas of northern kyrgyzstan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867107/
https://www.ncbi.nlm.nih.gov/pubmed/36675945
http://dx.doi.org/10.3390/jof9010124
work_keys_str_mv AT ozergoksel fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan
AT erperismail fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan
AT yıldızsenol fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan
AT bozoglutugba fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan
AT zholdoshbekovasezim fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan
AT alkanmehtap fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan
AT tekinfatih fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan
AT uulutairesenali fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan
AT imrenmustafa fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan
AT dababatabdelfattaha fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan
AT dervissibel fungalpathogensassociatedwithcrownandrootrotinwheatgrowingareasofnorthernkyrgyzstan