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Cytological and Molecular Mechanism of Low Pollen Grain Viability in a Germplasm Line of Double Lotus

Self-fertilization rate is an essential index of lotus reproductive system development, and pollen activity is a key factor affecting lotus seed setting rate. Based on cytology and molecular biology, this study addresses the main reasons for the low self-set rate of double lotus. It takes two differ...

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Autores principales: Liu, Guangyang, Liu, Fengjun, Jiang, Huiyan, Li, Jun, Jing, Jing, Jin, Qijiang, Wang, Yanjie, Qian, Ping, Xu, Yingchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867118/
https://www.ncbi.nlm.nih.gov/pubmed/36679100
http://dx.doi.org/10.3390/plants12020387
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author Liu, Guangyang
Liu, Fengjun
Jiang, Huiyan
Li, Jun
Jing, Jing
Jin, Qijiang
Wang, Yanjie
Qian, Ping
Xu, Yingchun
author_facet Liu, Guangyang
Liu, Fengjun
Jiang, Huiyan
Li, Jun
Jing, Jing
Jin, Qijiang
Wang, Yanjie
Qian, Ping
Xu, Yingchun
author_sort Liu, Guangyang
collection PubMed
description Self-fertilization rate is an essential index of lotus reproductive system development, and pollen activity is a key factor affecting lotus seed setting rate. Based on cytology and molecular biology, this study addresses the main reasons for the low self-set rate of double lotus. It takes two different double lotus breeds into consideration, namely ‘Sijingganshan’ with a low self-crossing rate and ‘Jinfurong’ with a high self-crossing rate. Cytological analysis results showed that the pollen abortion caused by excessive degradation of tapetum during the single phase was the root cause for the low self-mating rate of double lotus. Subsequent transcriptome analysis revealed that the gene NnPTC1 related to programmed tapetum cell death was significantly differentially expressed during the critical period of abortion, which further verified the specific expression of NnPTC1 in anthers. It was found that the expression level of NnPTC1 in ‘Sijingganshan’ at the mononuclear stage of its microspore development was significantly higher than that of ‘Jinfurong’ at the same stage. The overexpression of NnPTC1 resulted in the premature degradation of the tapetum and significantly decreased seed setting rate. These results indicated that the NnPTC1 gene regulated the pollen abortion of double lotus. The mechanism causing a low seed setting rate for double lotus was preliminarily revealed, which provided a theoretical basis for cultivating lotus varieties with both flower and seed.
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spelling pubmed-98671182023-01-22 Cytological and Molecular Mechanism of Low Pollen Grain Viability in a Germplasm Line of Double Lotus Liu, Guangyang Liu, Fengjun Jiang, Huiyan Li, Jun Jing, Jing Jin, Qijiang Wang, Yanjie Qian, Ping Xu, Yingchun Plants (Basel) Article Self-fertilization rate is an essential index of lotus reproductive system development, and pollen activity is a key factor affecting lotus seed setting rate. Based on cytology and molecular biology, this study addresses the main reasons for the low self-set rate of double lotus. It takes two different double lotus breeds into consideration, namely ‘Sijingganshan’ with a low self-crossing rate and ‘Jinfurong’ with a high self-crossing rate. Cytological analysis results showed that the pollen abortion caused by excessive degradation of tapetum during the single phase was the root cause for the low self-mating rate of double lotus. Subsequent transcriptome analysis revealed that the gene NnPTC1 related to programmed tapetum cell death was significantly differentially expressed during the critical period of abortion, which further verified the specific expression of NnPTC1 in anthers. It was found that the expression level of NnPTC1 in ‘Sijingganshan’ at the mononuclear stage of its microspore development was significantly higher than that of ‘Jinfurong’ at the same stage. The overexpression of NnPTC1 resulted in the premature degradation of the tapetum and significantly decreased seed setting rate. These results indicated that the NnPTC1 gene regulated the pollen abortion of double lotus. The mechanism causing a low seed setting rate for double lotus was preliminarily revealed, which provided a theoretical basis for cultivating lotus varieties with both flower and seed. MDPI 2023-01-13 /pmc/articles/PMC9867118/ /pubmed/36679100 http://dx.doi.org/10.3390/plants12020387 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Guangyang
Liu, Fengjun
Jiang, Huiyan
Li, Jun
Jing, Jing
Jin, Qijiang
Wang, Yanjie
Qian, Ping
Xu, Yingchun
Cytological and Molecular Mechanism of Low Pollen Grain Viability in a Germplasm Line of Double Lotus
title Cytological and Molecular Mechanism of Low Pollen Grain Viability in a Germplasm Line of Double Lotus
title_full Cytological and Molecular Mechanism of Low Pollen Grain Viability in a Germplasm Line of Double Lotus
title_fullStr Cytological and Molecular Mechanism of Low Pollen Grain Viability in a Germplasm Line of Double Lotus
title_full_unstemmed Cytological and Molecular Mechanism of Low Pollen Grain Viability in a Germplasm Line of Double Lotus
title_short Cytological and Molecular Mechanism of Low Pollen Grain Viability in a Germplasm Line of Double Lotus
title_sort cytological and molecular mechanism of low pollen grain viability in a germplasm line of double lotus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867118/
https://www.ncbi.nlm.nih.gov/pubmed/36679100
http://dx.doi.org/10.3390/plants12020387
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