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Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany

More than 40 human cases of severe encephalitis caused by Borna disease virus 1 (BoDV-1) have been reported to German health authorities. In an endemic region in southern Germany, we conducted the seroepidemiological BoSOT study (“BoDV-1 after solid-organ transplantation”) to assess whether there ar...

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Autores principales: Bauswein, Markus, Eidenschink, Lisa, Knoll, Gertrud, Neumann, Bernhard, Angstwurm, Klemens, Zoubaa, Saida, Riemenschneider, Markus J, Lampl, Benedikt M J, Pregler, Matthias, Niller, Hans Helmut, Jantsch, Jonathan, Gessner, André, Eberhardt, Yvonne, Huppertz, Gunnar, Schramm, Torsten, Kühn, Stefanie, Koller, Michael, Drasch, Thomas, Ehrl, Yvonne, Banas, Bernhard, Offner, Robert, Schmidt, Barbara, Banas, Miriam C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867173/
https://www.ncbi.nlm.nih.gov/pubmed/36680228
http://dx.doi.org/10.3390/v15010188
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author Bauswein, Markus
Eidenschink, Lisa
Knoll, Gertrud
Neumann, Bernhard
Angstwurm, Klemens
Zoubaa, Saida
Riemenschneider, Markus J
Lampl, Benedikt M J
Pregler, Matthias
Niller, Hans Helmut
Jantsch, Jonathan
Gessner, André
Eberhardt, Yvonne
Huppertz, Gunnar
Schramm, Torsten
Kühn, Stefanie
Koller, Michael
Drasch, Thomas
Ehrl, Yvonne
Banas, Bernhard
Offner, Robert
Schmidt, Barbara
Banas, Miriam C.
author_facet Bauswein, Markus
Eidenschink, Lisa
Knoll, Gertrud
Neumann, Bernhard
Angstwurm, Klemens
Zoubaa, Saida
Riemenschneider, Markus J
Lampl, Benedikt M J
Pregler, Matthias
Niller, Hans Helmut
Jantsch, Jonathan
Gessner, André
Eberhardt, Yvonne
Huppertz, Gunnar
Schramm, Torsten
Kühn, Stefanie
Koller, Michael
Drasch, Thomas
Ehrl, Yvonne
Banas, Bernhard
Offner, Robert
Schmidt, Barbara
Banas, Miriam C.
author_sort Bauswein, Markus
collection PubMed
description More than 40 human cases of severe encephalitis caused by Borna disease virus 1 (BoDV-1) have been reported to German health authorities. In an endemic region in southern Germany, we conducted the seroepidemiological BoSOT study (“BoDV-1 after solid-organ transplantation”) to assess whether there are undetected oligo- or asymptomatic courses of infection. A total of 216 healthy blood donors and 280 outpatients after solid organ transplantation were screened by a recombinant BoDV-1 ELISA followed by an indirect immunofluorescence assay (iIFA) as confirmatory test. For comparison, 288 serum and 258 cerebrospinal fluid (CSF) samples with a request for tick-borne encephalitis (TBE) diagnostics were analyzed for BoDV-1 infections. ELISA screening reactivity rates ranged from 3.5% to 18.6% depending on the cohort and the used ELISA antigen, but only one sample of a patient from the cohort with requested TBE diagnostics was confirmed to be positive for anti-BoDV-1-IgG by iIFA. In addition, the corresponding CSF sample of this patient with a three-week history of severe neurological disease tested positive for BoDV-1 RNA. Due to the iIFA results, all other results were interpreted as false-reactive in the ELISA screening. By linear serological epitope mapping, cross-reactions with human and bacterial proteins were identified as possible underlying mechanism for the false-reactive ELISA screening results. In conclusion, no oligo- or asymptomatic infections were detected in the studied cohorts. Serological tests based on a single recombinant BoDV-1 antigen should be interpreted with caution, and an iIFA should always be performed in addition.
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spelling pubmed-98671732023-01-22 Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany Bauswein, Markus Eidenschink, Lisa Knoll, Gertrud Neumann, Bernhard Angstwurm, Klemens Zoubaa, Saida Riemenschneider, Markus J Lampl, Benedikt M J Pregler, Matthias Niller, Hans Helmut Jantsch, Jonathan Gessner, André Eberhardt, Yvonne Huppertz, Gunnar Schramm, Torsten Kühn, Stefanie Koller, Michael Drasch, Thomas Ehrl, Yvonne Banas, Bernhard Offner, Robert Schmidt, Barbara Banas, Miriam C. Viruses Article More than 40 human cases of severe encephalitis caused by Borna disease virus 1 (BoDV-1) have been reported to German health authorities. In an endemic region in southern Germany, we conducted the seroepidemiological BoSOT study (“BoDV-1 after solid-organ transplantation”) to assess whether there are undetected oligo- or asymptomatic courses of infection. A total of 216 healthy blood donors and 280 outpatients after solid organ transplantation were screened by a recombinant BoDV-1 ELISA followed by an indirect immunofluorescence assay (iIFA) as confirmatory test. For comparison, 288 serum and 258 cerebrospinal fluid (CSF) samples with a request for tick-borne encephalitis (TBE) diagnostics were analyzed for BoDV-1 infections. ELISA screening reactivity rates ranged from 3.5% to 18.6% depending on the cohort and the used ELISA antigen, but only one sample of a patient from the cohort with requested TBE diagnostics was confirmed to be positive for anti-BoDV-1-IgG by iIFA. In addition, the corresponding CSF sample of this patient with a three-week history of severe neurological disease tested positive for BoDV-1 RNA. Due to the iIFA results, all other results were interpreted as false-reactive in the ELISA screening. By linear serological epitope mapping, cross-reactions with human and bacterial proteins were identified as possible underlying mechanism for the false-reactive ELISA screening results. In conclusion, no oligo- or asymptomatic infections were detected in the studied cohorts. Serological tests based on a single recombinant BoDV-1 antigen should be interpreted with caution, and an iIFA should always be performed in addition. MDPI 2023-01-09 /pmc/articles/PMC9867173/ /pubmed/36680228 http://dx.doi.org/10.3390/v15010188 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bauswein, Markus
Eidenschink, Lisa
Knoll, Gertrud
Neumann, Bernhard
Angstwurm, Klemens
Zoubaa, Saida
Riemenschneider, Markus J
Lampl, Benedikt M J
Pregler, Matthias
Niller, Hans Helmut
Jantsch, Jonathan
Gessner, André
Eberhardt, Yvonne
Huppertz, Gunnar
Schramm, Torsten
Kühn, Stefanie
Koller, Michael
Drasch, Thomas
Ehrl, Yvonne
Banas, Bernhard
Offner, Robert
Schmidt, Barbara
Banas, Miriam C.
Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany
title Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany
title_full Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany
title_fullStr Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany
title_full_unstemmed Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany
title_short Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany
title_sort human infections with borna disease virus 1 (bodv-1) primarily lead to severe encephalitis: further evidence from the seroepidemiological bosot study in an endemic region in southern germany
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867173/
https://www.ncbi.nlm.nih.gov/pubmed/36680228
http://dx.doi.org/10.3390/v15010188
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