Integrative Approaches Establish Colour Polymorphism in the Bamboo-Feeding Leafhopper Mukaria splendida Distant (Hemiptera: Cicadellidae) from India

SIMPLE SUMMARY: Authentic species-level identification is key to many fields of entomology, especially insect pest management and biosecurity. Bamboo, known as “Poor Man’s Timber” and “Green Gold”, is one of the fastest growing plants on earth. Bamboo and food grain crops (rice and wheat, for example) belong to the same family (Poaceae), so there is a chance of host shift because of global climate change and extensive cultivation. In the present study, the colour polymorphism of the bamboo-feeding leafhopper Mukaria splendida is highlighted, along with additional geographical distribution records. The integration of the morpho-molecular data confirmed that all the colour morphs belonged to the same species, M. splendida. ABSTRACT: The leafhopper species, Mukaria splendida Distant, is economically important due to itsstatus as a pest on bamboo and was recently reported to bea vector for phytoplasmal disease. Morphological identification is often difficult and requires a high level of taxonomic expertise, with misidentifications causing problematic false-positive/negative results. In this paper, colour polymorphism was recorded in the bamboo-feedingleafhopper M. splendida (Distant, 1908), which is a major insect pest in the bamboo ecosystem based on explorations conducted in different locations of India. Ten morphs were identified for each sex of M. splendida Distant based on the colour pattern on the pronotum and forewings. However, in view of the economic importance of the species, the morphological studies were integrated with the molecular data for the accurate identification of the species. The morphological characteristics and sequence results of the amplified product of the mitochondrial cytochrome oxidase subunit I (mtCOI) gene confirmed that all the morphs were M. splendida and the pairwise distance matrix showed a negligible genetic distance in the COI mtDNA gene. Simulated future predictions, along with detailed notes on the colour polymorphic forms with illustrations, and additional distribution records as well as thebiology of M. splendida were discussed in light of the available literature, all of which will aid the authentic identification of this species.