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Full-Length Model of SaCas9-sgRNA-DNA Complex in Cleavage State

Staphylococcus aureus Cas9 (SaCas9) is a widely used genome editing tool. Understanding its molecular mechanisms of DNA cleavage could effectively guide the engineering optimization of this system. Here, we determined the first cryo-electron microscopy structure of the SaCas9-sgRNA-DNA ternary compl...

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Detalles Bibliográficos
Autores principales:	Du, Wenhao, Zhu, Haixia, Qian, Jiaqiang, Xue, Dongmei, Zheng, Sen, Huang, Qiang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867433/ https://www.ncbi.nlm.nih.gov/pubmed/36674715 http://dx.doi.org/10.3390/ijms24021204

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