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Time-resolved RNA signatures of CD4+ T cells in Parkinson’s disease
Parkinson’s disease (PD) emerges as a complex, multifactorial disease. While there is increasing evidence that dysregulated T cells play a central role in PD pathogenesis, elucidation of the pathomechanical changes in related signaling is still in its beginnings. We employed time-resolved RNA expres...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867723/ https://www.ncbi.nlm.nih.gov/pubmed/36681665 http://dx.doi.org/10.1038/s41420-023-01333-0 |
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author | Diener, Caroline Hart, Martin Kehl, Tim Becker-Dorison, Anouck Tänzer, Tanja Schub, David Krammes, Lena Sester, Martina Keller, Andreas Unger, Marcus Walch-Rückheim, Barbara Lenhof, Hans-Peter Meese, Eckart |
author_facet | Diener, Caroline Hart, Martin Kehl, Tim Becker-Dorison, Anouck Tänzer, Tanja Schub, David Krammes, Lena Sester, Martina Keller, Andreas Unger, Marcus Walch-Rückheim, Barbara Lenhof, Hans-Peter Meese, Eckart |
author_sort | Diener, Caroline |
collection | PubMed |
description | Parkinson’s disease (PD) emerges as a complex, multifactorial disease. While there is increasing evidence that dysregulated T cells play a central role in PD pathogenesis, elucidation of the pathomechanical changes in related signaling is still in its beginnings. We employed time-resolved RNA expression upon the activation of peripheral CD4+ T cells to track and functionally relate changes on cellular signaling in representative cases of patients at different stages of PD. While only few miRNAs showed time-course related expression changes in PD, we identified groups of genes with significantly altered expression for each different time window. Towards a further understanding of the functional consequences, we highlighted pathways with decreased or increased activity in PD, including the most prominent altered IL-17 pathway. Flow cytometric analyses showed not only an increased prevalence of Th17 cells but also a specific subtype of IL-17 producing γδ-T cells, indicating a previously unknown role in PD pathogenesis. |
format | Online Article Text |
id | pubmed-9867723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-98677232023-01-23 Time-resolved RNA signatures of CD4+ T cells in Parkinson’s disease Diener, Caroline Hart, Martin Kehl, Tim Becker-Dorison, Anouck Tänzer, Tanja Schub, David Krammes, Lena Sester, Martina Keller, Andreas Unger, Marcus Walch-Rückheim, Barbara Lenhof, Hans-Peter Meese, Eckart Cell Death Discov Article Parkinson’s disease (PD) emerges as a complex, multifactorial disease. While there is increasing evidence that dysregulated T cells play a central role in PD pathogenesis, elucidation of the pathomechanical changes in related signaling is still in its beginnings. We employed time-resolved RNA expression upon the activation of peripheral CD4+ T cells to track and functionally relate changes on cellular signaling in representative cases of patients at different stages of PD. While only few miRNAs showed time-course related expression changes in PD, we identified groups of genes with significantly altered expression for each different time window. Towards a further understanding of the functional consequences, we highlighted pathways with decreased or increased activity in PD, including the most prominent altered IL-17 pathway. Flow cytometric analyses showed not only an increased prevalence of Th17 cells but also a specific subtype of IL-17 producing γδ-T cells, indicating a previously unknown role in PD pathogenesis. Nature Publishing Group UK 2023-01-21 /pmc/articles/PMC9867723/ /pubmed/36681665 http://dx.doi.org/10.1038/s41420-023-01333-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Diener, Caroline Hart, Martin Kehl, Tim Becker-Dorison, Anouck Tänzer, Tanja Schub, David Krammes, Lena Sester, Martina Keller, Andreas Unger, Marcus Walch-Rückheim, Barbara Lenhof, Hans-Peter Meese, Eckart Time-resolved RNA signatures of CD4+ T cells in Parkinson’s disease |
title | Time-resolved RNA signatures of CD4+ T cells in Parkinson’s disease |
title_full | Time-resolved RNA signatures of CD4+ T cells in Parkinson’s disease |
title_fullStr | Time-resolved RNA signatures of CD4+ T cells in Parkinson’s disease |
title_full_unstemmed | Time-resolved RNA signatures of CD4+ T cells in Parkinson’s disease |
title_short | Time-resolved RNA signatures of CD4+ T cells in Parkinson’s disease |
title_sort | time-resolved rna signatures of cd4+ t cells in parkinson’s disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867723/ https://www.ncbi.nlm.nih.gov/pubmed/36681665 http://dx.doi.org/10.1038/s41420-023-01333-0 |
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