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Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)

This study devised a dual-species biofilm model to investigate bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) simultaneously, and compared the efficacy of conventional and contemporary disinfection protocols. Seventy single-rooted mandibular premolars were included. Fourteen teeth w...

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Autores principales: Alquria, Theeb A., Alfirdous, Rayyan A., Gupta, Swati, Santamaria, Mauro P., Santamaria, Ingrid F., Gomes, Ana P. M., Tiradentes, Naiara, Silva, Eduardo G., Martinho, Frederico C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867776/
https://www.ncbi.nlm.nih.gov/pubmed/36681702
http://dx.doi.org/10.1038/s41598-022-26855-y
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author Alquria, Theeb A.
Alfirdous, Rayyan A.
Gupta, Swati
Santamaria, Mauro P.
Santamaria, Ingrid F.
Gomes, Ana P. M.
Tiradentes, Naiara
Silva, Eduardo G.
Martinho, Frederico C.
author_facet Alquria, Theeb A.
Alfirdous, Rayyan A.
Gupta, Swati
Santamaria, Mauro P.
Santamaria, Ingrid F.
Gomes, Ana P. M.
Tiradentes, Naiara
Silva, Eduardo G.
Martinho, Frederico C.
author_sort Alquria, Theeb A.
collection PubMed
description This study devised a dual-species biofilm model to investigate bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) simultaneously, and compared the efficacy of conventional and contemporary disinfection protocols. Seventy single-rooted mandibular premolars were included. Fourteen teeth were negative control, and 56 teeth were infected with 3-week-old E. faecalis and E. coli GFP biofilm. Fourteen/56 teeth were positive control, with seven teeth processed for CLSM analysis and seven teeth sampled with paper points (PPs) and cryogenically ground for bacterial, LTA, and LPS analyses. Forty-two teeth were randomly divided into three groups: GWS (GentleWave system) + MIT (minimally invasive technique), PUI (passive ultrasonic irrigation) + CIT (conventional instrumentation technique), and XP-EF (XP-endo Finisher) + CIT (All, n = 14). Samples were collected before (s1) and after disinfection (s2) with PPs and after cryogenically ground (s3). CFUs were counted, and LTA and LPS were quantified with LTA-ELISA and LAL assay, respectively. XP-EF was as effective as PUI (p > 0.05). GWS + MIT was the most effective disinfection protocol against bacteria, LTA, and LPS (p < 0.05). In conclusion, PUI, XP-EF, and GWS were highly effective against bacteria, LTA, and LPS, with GWS being the most effective.
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spelling pubmed-98677762023-01-23 Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) Alquria, Theeb A. Alfirdous, Rayyan A. Gupta, Swati Santamaria, Mauro P. Santamaria, Ingrid F. Gomes, Ana P. M. Tiradentes, Naiara Silva, Eduardo G. Martinho, Frederico C. Sci Rep Article This study devised a dual-species biofilm model to investigate bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) simultaneously, and compared the efficacy of conventional and contemporary disinfection protocols. Seventy single-rooted mandibular premolars were included. Fourteen teeth were negative control, and 56 teeth were infected with 3-week-old E. faecalis and E. coli GFP biofilm. Fourteen/56 teeth were positive control, with seven teeth processed for CLSM analysis and seven teeth sampled with paper points (PPs) and cryogenically ground for bacterial, LTA, and LPS analyses. Forty-two teeth were randomly divided into three groups: GWS (GentleWave system) + MIT (minimally invasive technique), PUI (passive ultrasonic irrigation) + CIT (conventional instrumentation technique), and XP-EF (XP-endo Finisher) + CIT (All, n = 14). Samples were collected before (s1) and after disinfection (s2) with PPs and after cryogenically ground (s3). CFUs were counted, and LTA and LPS were quantified with LTA-ELISA and LAL assay, respectively. XP-EF was as effective as PUI (p > 0.05). GWS + MIT was the most effective disinfection protocol against bacteria, LTA, and LPS (p < 0.05). In conclusion, PUI, XP-EF, and GWS were highly effective against bacteria, LTA, and LPS, with GWS being the most effective. Nature Publishing Group UK 2023-01-21 /pmc/articles/PMC9867776/ /pubmed/36681702 http://dx.doi.org/10.1038/s41598-022-26855-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Alquria, Theeb A.
Alfirdous, Rayyan A.
Gupta, Swati
Santamaria, Mauro P.
Santamaria, Ingrid F.
Gomes, Ana P. M.
Tiradentes, Naiara
Silva, Eduardo G.
Martinho, Frederico C.
Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)
title Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)
title_full Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)
title_fullStr Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)
title_full_unstemmed Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)
title_short Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)
title_sort comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (lta), and lipopolysaccharide (lps)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867776/
https://www.ncbi.nlm.nih.gov/pubmed/36681702
http://dx.doi.org/10.1038/s41598-022-26855-y
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