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Sex-dependent effects of microglial reduction on impaired fear extinction induced by single prolonged stress

Single prolonged stress (SPS) is a preclinical rodent model for studying post-traumatic stress disorder (PTSD)-like behaviors. Previously we found that increased expression of the microglial marker Iba-1 in the ventral hippocampus after SPS exposure was associated with impaired fear extinction, sugg...

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Autores principales: Torres-Rodriguez, Orlando, Ortiz-Nazario, Emily, Rivera-Escobales, Yesenia, Velazquez, Bethzaly, Colón, María, Porter, James T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868263/
https://www.ncbi.nlm.nih.gov/pubmed/36699653
http://dx.doi.org/10.3389/fnbeh.2022.1014767
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author Torres-Rodriguez, Orlando
Ortiz-Nazario, Emily
Rivera-Escobales, Yesenia
Velazquez, Bethzaly
Colón, María
Porter, James T.
author_facet Torres-Rodriguez, Orlando
Ortiz-Nazario, Emily
Rivera-Escobales, Yesenia
Velazquez, Bethzaly
Colón, María
Porter, James T.
author_sort Torres-Rodriguez, Orlando
collection PubMed
description Single prolonged stress (SPS) is a preclinical rodent model for studying post-traumatic stress disorder (PTSD)-like behaviors. Previously we found that increased expression of the microglial marker Iba-1 in the ventral hippocampus after SPS exposure was associated with impaired fear extinction, suggesting that microglial activity contributed to the SPS-induced behavioral changes. To test this, we examined whether reducing microglia with the colony-stimulating factor 1 receptor blocker, PLX3397, in the diet would prevent the SPS-induced extinction impairment. Male rats exposed to SPS showed enhanced fear acquisition and impaired fear extinction memory. Adding PLX3397 to the diet prevented these behavioral changes. In contrast, PLX3397 did not prevent SPS from impairing fear extinction memory in the female rats. Despite the sex-dependent behavioral effects, we found a reduced number and area fraction of Iba-1+ microglia in both male and female rats suggesting that PLX3397 had similar effects on microglia in both sexes. Altogether, these results suggest that microglia contribute to the behavioral changes induced by SPS in male but not female rats.
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spelling pubmed-98682632023-01-24 Sex-dependent effects of microglial reduction on impaired fear extinction induced by single prolonged stress Torres-Rodriguez, Orlando Ortiz-Nazario, Emily Rivera-Escobales, Yesenia Velazquez, Bethzaly Colón, María Porter, James T. Front Behav Neurosci Behavioral Neuroscience Single prolonged stress (SPS) is a preclinical rodent model for studying post-traumatic stress disorder (PTSD)-like behaviors. Previously we found that increased expression of the microglial marker Iba-1 in the ventral hippocampus after SPS exposure was associated with impaired fear extinction, suggesting that microglial activity contributed to the SPS-induced behavioral changes. To test this, we examined whether reducing microglia with the colony-stimulating factor 1 receptor blocker, PLX3397, in the diet would prevent the SPS-induced extinction impairment. Male rats exposed to SPS showed enhanced fear acquisition and impaired fear extinction memory. Adding PLX3397 to the diet prevented these behavioral changes. In contrast, PLX3397 did not prevent SPS from impairing fear extinction memory in the female rats. Despite the sex-dependent behavioral effects, we found a reduced number and area fraction of Iba-1+ microglia in both male and female rats suggesting that PLX3397 had similar effects on microglia in both sexes. Altogether, these results suggest that microglia contribute to the behavioral changes induced by SPS in male but not female rats. Frontiers Media S.A. 2023-01-09 /pmc/articles/PMC9868263/ /pubmed/36699653 http://dx.doi.org/10.3389/fnbeh.2022.1014767 Text en Copyright © 2023 Torres-Rodriguez, Ortiz-Nazario, Rivera-Escobales, Velazquez, Colón and Porter. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Behavioral Neuroscience
Torres-Rodriguez, Orlando
Ortiz-Nazario, Emily
Rivera-Escobales, Yesenia
Velazquez, Bethzaly
Colón, María
Porter, James T.
Sex-dependent effects of microglial reduction on impaired fear extinction induced by single prolonged stress
title Sex-dependent effects of microglial reduction on impaired fear extinction induced by single prolonged stress
title_full Sex-dependent effects of microglial reduction on impaired fear extinction induced by single prolonged stress
title_fullStr Sex-dependent effects of microglial reduction on impaired fear extinction induced by single prolonged stress
title_full_unstemmed Sex-dependent effects of microglial reduction on impaired fear extinction induced by single prolonged stress
title_short Sex-dependent effects of microglial reduction on impaired fear extinction induced by single prolonged stress
title_sort sex-dependent effects of microglial reduction on impaired fear extinction induced by single prolonged stress
topic Behavioral Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868263/
https://www.ncbi.nlm.nih.gov/pubmed/36699653
http://dx.doi.org/10.3389/fnbeh.2022.1014767
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