Cargando…

Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner

INTRODUCTION: Autophagy and endocytosis are crucial biological activities in mammalian follicle development and oocyte maturation, which are easily affected by external environmental factors. Epidermal growth factor (EGF), as an important component of follicular fluid, regulates the growth and apopt...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Rui, Yu, Sijiu, Cui, Yan, Pan, Yangyang, Wang, Meng, Wang, Libin, Wang, Jinglei, Zhao, Ling, Zhang, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868291/
https://www.ncbi.nlm.nih.gov/pubmed/36699336
http://dx.doi.org/10.3389/fvets.2022.1081643
_version_ 1784876501246672896
author Ma, Rui
Yu, Sijiu
Cui, Yan
Pan, Yangyang
Wang, Meng
Wang, Libin
Wang, Jinglei
Zhao, Ling
Zhang, Hui
author_facet Ma, Rui
Yu, Sijiu
Cui, Yan
Pan, Yangyang
Wang, Meng
Wang, Libin
Wang, Jinglei
Zhao, Ling
Zhang, Hui
author_sort Ma, Rui
collection PubMed
description INTRODUCTION: Autophagy and endocytosis are crucial biological activities in mammalian follicle development and oocyte maturation, which are easily affected by external environmental factors. Epidermal growth factor (EGF), as an important component of follicular fluid, regulates the growth and apoptosis of follicular cells. However, its regulatory mechanism of autophagy and endocytosis in mammals, especially in large domestic animals such as plateau yak, remains unclear. This study aimed to investigate the regulatory mechanism of EGF on autophagy and endocytosis in yak cumulus cells. METHODS: Yak cumulus cells were treated with different concentrations of EGF and appropriate concentrations of EGFR inhibitor gefitinib (10 μM). The dynamic expression levels of Atg5, Beclin1, LC3, Cav1 and Cav2 were detected by immunofluorescence staining, qRT-PCR and Western-blot. RESULTS: EGF inhibited autophagy in yak cumulus cells by down-regulating the expression of Atg5, Beclin1, and LC3. The level of autophagy varied with the concentration of ligands, and the inhibition was most significant at 100 ng/mL. Noteworthy, EGF can promote endocytosis by regulating the expression of Cav1 and Cav2, but the EGFR-mediated signaling pathway is not the main way to regulate the expression of these proteins. DISCUSSION: These results provide a reference for further exploring the effects of growth factors on livestock germ cells and the regulatory role of autophagy-endocytosis crosstalk mechanism in follicle development and oocyte maturation, to improve the fecundity of yaks.
format Online
Article
Text
id pubmed-9868291
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98682912023-01-24 Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner Ma, Rui Yu, Sijiu Cui, Yan Pan, Yangyang Wang, Meng Wang, Libin Wang, Jinglei Zhao, Ling Zhang, Hui Front Vet Sci Veterinary Science INTRODUCTION: Autophagy and endocytosis are crucial biological activities in mammalian follicle development and oocyte maturation, which are easily affected by external environmental factors. Epidermal growth factor (EGF), as an important component of follicular fluid, regulates the growth and apoptosis of follicular cells. However, its regulatory mechanism of autophagy and endocytosis in mammals, especially in large domestic animals such as plateau yak, remains unclear. This study aimed to investigate the regulatory mechanism of EGF on autophagy and endocytosis in yak cumulus cells. METHODS: Yak cumulus cells were treated with different concentrations of EGF and appropriate concentrations of EGFR inhibitor gefitinib (10 μM). The dynamic expression levels of Atg5, Beclin1, LC3, Cav1 and Cav2 were detected by immunofluorescence staining, qRT-PCR and Western-blot. RESULTS: EGF inhibited autophagy in yak cumulus cells by down-regulating the expression of Atg5, Beclin1, and LC3. The level of autophagy varied with the concentration of ligands, and the inhibition was most significant at 100 ng/mL. Noteworthy, EGF can promote endocytosis by regulating the expression of Cav1 and Cav2, but the EGFR-mediated signaling pathway is not the main way to regulate the expression of these proteins. DISCUSSION: These results provide a reference for further exploring the effects of growth factors on livestock germ cells and the regulatory role of autophagy-endocytosis crosstalk mechanism in follicle development and oocyte maturation, to improve the fecundity of yaks. Frontiers Media S.A. 2023-01-09 /pmc/articles/PMC9868291/ /pubmed/36699336 http://dx.doi.org/10.3389/fvets.2022.1081643 Text en Copyright © 2023 Ma, Yu, Cui, Pan, Wang, Wang, Wang, Zhao and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Ma, Rui
Yu, Sijiu
Cui, Yan
Pan, Yangyang
Wang, Meng
Wang, Libin
Wang, Jinglei
Zhao, Ling
Zhang, Hui
Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner
title Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner
title_full Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner
title_fullStr Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner
title_full_unstemmed Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner
title_short Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner
title_sort epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868291/
https://www.ncbi.nlm.nih.gov/pubmed/36699336
http://dx.doi.org/10.3389/fvets.2022.1081643
work_keys_str_mv AT marui epidermalgrowthfactorregulatesautophagyactivityandendocytosisofyakcumuluscellsinaconcentrationdependentmanner
AT yusijiu epidermalgrowthfactorregulatesautophagyactivityandendocytosisofyakcumuluscellsinaconcentrationdependentmanner
AT cuiyan epidermalgrowthfactorregulatesautophagyactivityandendocytosisofyakcumuluscellsinaconcentrationdependentmanner
AT panyangyang epidermalgrowthfactorregulatesautophagyactivityandendocytosisofyakcumuluscellsinaconcentrationdependentmanner
AT wangmeng epidermalgrowthfactorregulatesautophagyactivityandendocytosisofyakcumuluscellsinaconcentrationdependentmanner
AT wanglibin epidermalgrowthfactorregulatesautophagyactivityandendocytosisofyakcumuluscellsinaconcentrationdependentmanner
AT wangjinglei epidermalgrowthfactorregulatesautophagyactivityandendocytosisofyakcumuluscellsinaconcentrationdependentmanner
AT zhaoling epidermalgrowthfactorregulatesautophagyactivityandendocytosisofyakcumuluscellsinaconcentrationdependentmanner
AT zhanghui epidermalgrowthfactorregulatesautophagyactivityandendocytosisofyakcumuluscellsinaconcentrationdependentmanner