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Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner
INTRODUCTION: Autophagy and endocytosis are crucial biological activities in mammalian follicle development and oocyte maturation, which are easily affected by external environmental factors. Epidermal growth factor (EGF), as an important component of follicular fluid, regulates the growth and apopt...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868291/ https://www.ncbi.nlm.nih.gov/pubmed/36699336 http://dx.doi.org/10.3389/fvets.2022.1081643 |
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author | Ma, Rui Yu, Sijiu Cui, Yan Pan, Yangyang Wang, Meng Wang, Libin Wang, Jinglei Zhao, Ling Zhang, Hui |
author_facet | Ma, Rui Yu, Sijiu Cui, Yan Pan, Yangyang Wang, Meng Wang, Libin Wang, Jinglei Zhao, Ling Zhang, Hui |
author_sort | Ma, Rui |
collection | PubMed |
description | INTRODUCTION: Autophagy and endocytosis are crucial biological activities in mammalian follicle development and oocyte maturation, which are easily affected by external environmental factors. Epidermal growth factor (EGF), as an important component of follicular fluid, regulates the growth and apoptosis of follicular cells. However, its regulatory mechanism of autophagy and endocytosis in mammals, especially in large domestic animals such as plateau yak, remains unclear. This study aimed to investigate the regulatory mechanism of EGF on autophagy and endocytosis in yak cumulus cells. METHODS: Yak cumulus cells were treated with different concentrations of EGF and appropriate concentrations of EGFR inhibitor gefitinib (10 μM). The dynamic expression levels of Atg5, Beclin1, LC3, Cav1 and Cav2 were detected by immunofluorescence staining, qRT-PCR and Western-blot. RESULTS: EGF inhibited autophagy in yak cumulus cells by down-regulating the expression of Atg5, Beclin1, and LC3. The level of autophagy varied with the concentration of ligands, and the inhibition was most significant at 100 ng/mL. Noteworthy, EGF can promote endocytosis by regulating the expression of Cav1 and Cav2, but the EGFR-mediated signaling pathway is not the main way to regulate the expression of these proteins. DISCUSSION: These results provide a reference for further exploring the effects of growth factors on livestock germ cells and the regulatory role of autophagy-endocytosis crosstalk mechanism in follicle development and oocyte maturation, to improve the fecundity of yaks. |
format | Online Article Text |
id | pubmed-9868291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98682912023-01-24 Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner Ma, Rui Yu, Sijiu Cui, Yan Pan, Yangyang Wang, Meng Wang, Libin Wang, Jinglei Zhao, Ling Zhang, Hui Front Vet Sci Veterinary Science INTRODUCTION: Autophagy and endocytosis are crucial biological activities in mammalian follicle development and oocyte maturation, which are easily affected by external environmental factors. Epidermal growth factor (EGF), as an important component of follicular fluid, regulates the growth and apoptosis of follicular cells. However, its regulatory mechanism of autophagy and endocytosis in mammals, especially in large domestic animals such as plateau yak, remains unclear. This study aimed to investigate the regulatory mechanism of EGF on autophagy and endocytosis in yak cumulus cells. METHODS: Yak cumulus cells were treated with different concentrations of EGF and appropriate concentrations of EGFR inhibitor gefitinib (10 μM). The dynamic expression levels of Atg5, Beclin1, LC3, Cav1 and Cav2 were detected by immunofluorescence staining, qRT-PCR and Western-blot. RESULTS: EGF inhibited autophagy in yak cumulus cells by down-regulating the expression of Atg5, Beclin1, and LC3. The level of autophagy varied with the concentration of ligands, and the inhibition was most significant at 100 ng/mL. Noteworthy, EGF can promote endocytosis by regulating the expression of Cav1 and Cav2, but the EGFR-mediated signaling pathway is not the main way to regulate the expression of these proteins. DISCUSSION: These results provide a reference for further exploring the effects of growth factors on livestock germ cells and the regulatory role of autophagy-endocytosis crosstalk mechanism in follicle development and oocyte maturation, to improve the fecundity of yaks. Frontiers Media S.A. 2023-01-09 /pmc/articles/PMC9868291/ /pubmed/36699336 http://dx.doi.org/10.3389/fvets.2022.1081643 Text en Copyright © 2023 Ma, Yu, Cui, Pan, Wang, Wang, Wang, Zhao and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Ma, Rui Yu, Sijiu Cui, Yan Pan, Yangyang Wang, Meng Wang, Libin Wang, Jinglei Zhao, Ling Zhang, Hui Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner |
title | Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner |
title_full | Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner |
title_fullStr | Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner |
title_full_unstemmed | Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner |
title_short | Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner |
title_sort | epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868291/ https://www.ncbi.nlm.nih.gov/pubmed/36699336 http://dx.doi.org/10.3389/fvets.2022.1081643 |
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