Twisting and swiveling domain motions in Cas9 to recognize target DNA duplexes, make double-strand breaks, and release cleaved duplexes

The CRISPR-associated protein 9 (Cas9) has been engineered as a precise gene editing tool to make double-strand breaks. CRISPR-associated protein 9 binds the folded guide RNA (gRNA) that serves as a binding scaffold to guide it to the target DNA duplex via a RecA-like strand-displacement mechanism b...

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Detalles Bibliográficos
Autores principales: Wang, Jimin, Arantes, Pablo R., Ahsan, Mohd, Sinha, Souvik, Kyro, Gregory W., Maschietto, Federica, Allen, Brandon, Skeens, Erin, Lisi, George P., Batista, Victor S., Palermo, Giulia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868570/
https://www.ncbi.nlm.nih.gov/pubmed/36699705
http://dx.doi.org/10.3389/fmolb.2022.1072733

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