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Advanced strategies for production of soy-processing enzyme

Enzyme production is critical and often costly for biorefinery. It is challenging to produce enzymes with not only high titers but also proper combinations of all required activities in a single fermentation. This work aimed at improving productivity and composition of the multiple enzyme activities...

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Detalles Bibliográficos
Autores principales: Islam, S. M. Mahfuzul, Ju, Lu-Kwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868571/
https://www.ncbi.nlm.nih.gov/pubmed/36698638
http://dx.doi.org/10.3389/fbioe.2022.1042001
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author Islam, S. M. Mahfuzul
Ju, Lu-Kwang
author_facet Islam, S. M. Mahfuzul
Ju, Lu-Kwang
author_sort Islam, S. M. Mahfuzul
collection PubMed
description Enzyme production is critical and often costly for biorefinery. It is challenging to produce enzymes with not only high titers but also proper combinations of all required activities in a single fermentation. This work aimed at improving productivity and composition of the multiple enzyme activities required for hydrolysis of complex soybean carbohydrate in a single fermentation. A previously selected Aspergillus niger strain was used for its high carbohydrases and low protease production. Strategies of fed-batch substrate addition and programmed pH-decrease rates were evaluated. Cheap soybean hull (SH) was confirmed to induce production of all necessary carbohydrases. Surprisingly, fed-batch SH addition, originally thought to sustain substrate-inducer availability and reduce feedback repression by sugars, did not increase pectinase and cellulase production significantly and even lowered the α-galactosidase production, when compared with batch fermentation having the same total SH amount (all added initially). On the other hand, the pH-decrease rate could be effectively optimized for production of complex enzyme mixtures. The best fermentation was programmed to lower pH from 7 to 4 in 84 h, at a drop rate of .0357 per h. It produced the highest pectinase (19.1 ± .04 U/mL), α-galactosidase (15.7 ± .4 U/mL), and cellulase (.88 ± .06 FPU/mL). Producing these high enzyme activities in a single fermentation significantly improves the effectiveness and economics of enzymatic soy processing, which, e.g., can hydrolyze the 30%–35% carbohydrate in soybean meal to sugars, with minimal protein degradation, to generate high-value protein-rich products and a hydrolysate as fermentation feedstock.
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spelling pubmed-98685712023-01-24 Advanced strategies for production of soy-processing enzyme Islam, S. M. Mahfuzul Ju, Lu-Kwang Front Bioeng Biotechnol Bioengineering and Biotechnology Enzyme production is critical and often costly for biorefinery. It is challenging to produce enzymes with not only high titers but also proper combinations of all required activities in a single fermentation. This work aimed at improving productivity and composition of the multiple enzyme activities required for hydrolysis of complex soybean carbohydrate in a single fermentation. A previously selected Aspergillus niger strain was used for its high carbohydrases and low protease production. Strategies of fed-batch substrate addition and programmed pH-decrease rates were evaluated. Cheap soybean hull (SH) was confirmed to induce production of all necessary carbohydrases. Surprisingly, fed-batch SH addition, originally thought to sustain substrate-inducer availability and reduce feedback repression by sugars, did not increase pectinase and cellulase production significantly and even lowered the α-galactosidase production, when compared with batch fermentation having the same total SH amount (all added initially). On the other hand, the pH-decrease rate could be effectively optimized for production of complex enzyme mixtures. The best fermentation was programmed to lower pH from 7 to 4 in 84 h, at a drop rate of .0357 per h. It produced the highest pectinase (19.1 ± .04 U/mL), α-galactosidase (15.7 ± .4 U/mL), and cellulase (.88 ± .06 FPU/mL). Producing these high enzyme activities in a single fermentation significantly improves the effectiveness and economics of enzymatic soy processing, which, e.g., can hydrolyze the 30%–35% carbohydrate in soybean meal to sugars, with minimal protein degradation, to generate high-value protein-rich products and a hydrolysate as fermentation feedstock. Frontiers Media S.A. 2023-01-09 /pmc/articles/PMC9868571/ /pubmed/36698638 http://dx.doi.org/10.3389/fbioe.2022.1042001 Text en Copyright © 2023 Islam and Ju. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Islam, S. M. Mahfuzul
Ju, Lu-Kwang
Advanced strategies for production of soy-processing enzyme
title Advanced strategies for production of soy-processing enzyme
title_full Advanced strategies for production of soy-processing enzyme
title_fullStr Advanced strategies for production of soy-processing enzyme
title_full_unstemmed Advanced strategies for production of soy-processing enzyme
title_short Advanced strategies for production of soy-processing enzyme
title_sort advanced strategies for production of soy-processing enzyme
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868571/
https://www.ncbi.nlm.nih.gov/pubmed/36698638
http://dx.doi.org/10.3389/fbioe.2022.1042001
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