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Hemorheological profiles and chronic inflammation markers in transfusion-dependent and non-transfusion- dependent thalassemia

The rheological properties of blood play an important role in regulating blood flow in micro and macro circulation. In thalassemia syndromes red blood cells exhibit altered hemodynamic properties that facilitate microcirculatory diseases: increased aggregation and reduced deformability, as well as a...

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Autores principales: Caprari, Patrizia, Profumo, Elisabetta, Massimi, Sara, Buttari, Brigitta, Riganò, Rachele, Regine, Vincenza, Gabbianelli, Marco, Rossi, Stefania, Risoluti, Roberta, Materazzi, Stefano, Gullifa, Giuseppina, Maffei, Laura, Sorrentino, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868635/
https://www.ncbi.nlm.nih.gov/pubmed/36699704
http://dx.doi.org/10.3389/fmolb.2022.1108896
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author Caprari, Patrizia
Profumo, Elisabetta
Massimi, Sara
Buttari, Brigitta
Riganò, Rachele
Regine, Vincenza
Gabbianelli, Marco
Rossi, Stefania
Risoluti, Roberta
Materazzi, Stefano
Gullifa, Giuseppina
Maffei, Laura
Sorrentino, Francesco
author_facet Caprari, Patrizia
Profumo, Elisabetta
Massimi, Sara
Buttari, Brigitta
Riganò, Rachele
Regine, Vincenza
Gabbianelli, Marco
Rossi, Stefania
Risoluti, Roberta
Materazzi, Stefano
Gullifa, Giuseppina
Maffei, Laura
Sorrentino, Francesco
author_sort Caprari, Patrizia
collection PubMed
description The rheological properties of blood play an important role in regulating blood flow in micro and macro circulation. In thalassemia syndromes red blood cells exhibit altered hemodynamic properties that facilitate microcirculatory diseases: increased aggregation and reduced deformability, as well as a marked increase in adherence to the vascular endothelial cells. A personalized approach to treating thalassemia patients (transfusions, iron chelation, and splenectomy), has increased patients’ life expectancy, however they generally present many complications and several studies have demonstrated the presence of high incidence of thromboembolic events. In this study the hemorheological profiles of thalassemia patients have been characterized to point out new indices of vascular impairment in thalassemia. Plasma viscosity, blood viscosities at low and high shear rates (η1 and η200, respectively), erythrocyte aggregation index (η1/η200), and the erythrocyte viscoelastic profile (elastic modulus G', and viscous modulus G") have been studied in transfusion-dependent and non-transfusion-dependent thalassemia patients. Moreover, the levels of inflammation biomarkers in thalassemia have been evaluated to investigate a relationship between the biomarkers, the disease severity and the rheological parameters. The biomarkers studied are the main components of the immune and endothelial systems or are related to vascular inflammation: cytokines (IL-2, IL-6, IL-10, IL-17A, TNF-alpha), chemokines (IL-8, MIP-1alpha), adipocytokines (leptin and adiponectin), growth factors (VEGF, angiopoietin-1), adhesion molecules (ICAM-1, VCAM-1, E-selectin, L-selectin), and a monocyte/macrophage activation marker (CD163). This study shows that transfusion-dependent thalassemia patients, both major and intermedia, have blood viscosities comparable to those of healthy subjects. Non-transfusion-dependent thalassemia intermedia patients show high blood viscosities at low shear rates (η1), corresponding to the flow conditions of the microcirculation, an increase in erythrocyte aggregation, and high values of the elastic G' and viscous G" modules that reflect a reduced erythrocyte deformability and an increase in blood viscosity. Levels of cytokines, chemokines and adhesion molecules are different in transfusion- and non-transfusion dependent patients and positive correlations between η1 or η1/η200 and the cytokines IL-6 and IL-10 have been observed. The evaluation of the hemorheological profiles in thalassemia can provide new indicators of vascular impairment and disease severity in thalassemia in order to prevent the onset of thromboembolic events.
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spelling pubmed-98686352023-01-24 Hemorheological profiles and chronic inflammation markers in transfusion-dependent and non-transfusion- dependent thalassemia Caprari, Patrizia Profumo, Elisabetta Massimi, Sara Buttari, Brigitta Riganò, Rachele Regine, Vincenza Gabbianelli, Marco Rossi, Stefania Risoluti, Roberta Materazzi, Stefano Gullifa, Giuseppina Maffei, Laura Sorrentino, Francesco Front Mol Biosci Molecular Biosciences The rheological properties of blood play an important role in regulating blood flow in micro and macro circulation. In thalassemia syndromes red blood cells exhibit altered hemodynamic properties that facilitate microcirculatory diseases: increased aggregation and reduced deformability, as well as a marked increase in adherence to the vascular endothelial cells. A personalized approach to treating thalassemia patients (transfusions, iron chelation, and splenectomy), has increased patients’ life expectancy, however they generally present many complications and several studies have demonstrated the presence of high incidence of thromboembolic events. In this study the hemorheological profiles of thalassemia patients have been characterized to point out new indices of vascular impairment in thalassemia. Plasma viscosity, blood viscosities at low and high shear rates (η1 and η200, respectively), erythrocyte aggregation index (η1/η200), and the erythrocyte viscoelastic profile (elastic modulus G', and viscous modulus G") have been studied in transfusion-dependent and non-transfusion-dependent thalassemia patients. Moreover, the levels of inflammation biomarkers in thalassemia have been evaluated to investigate a relationship between the biomarkers, the disease severity and the rheological parameters. The biomarkers studied are the main components of the immune and endothelial systems or are related to vascular inflammation: cytokines (IL-2, IL-6, IL-10, IL-17A, TNF-alpha), chemokines (IL-8, MIP-1alpha), adipocytokines (leptin and adiponectin), growth factors (VEGF, angiopoietin-1), adhesion molecules (ICAM-1, VCAM-1, E-selectin, L-selectin), and a monocyte/macrophage activation marker (CD163). This study shows that transfusion-dependent thalassemia patients, both major and intermedia, have blood viscosities comparable to those of healthy subjects. Non-transfusion-dependent thalassemia intermedia patients show high blood viscosities at low shear rates (η1), corresponding to the flow conditions of the microcirculation, an increase in erythrocyte aggregation, and high values of the elastic G' and viscous G" modules that reflect a reduced erythrocyte deformability and an increase in blood viscosity. Levels of cytokines, chemokines and adhesion molecules are different in transfusion- and non-transfusion dependent patients and positive correlations between η1 or η1/η200 and the cytokines IL-6 and IL-10 have been observed. The evaluation of the hemorheological profiles in thalassemia can provide new indicators of vascular impairment and disease severity in thalassemia in order to prevent the onset of thromboembolic events. Frontiers Media S.A. 2023-01-09 /pmc/articles/PMC9868635/ /pubmed/36699704 http://dx.doi.org/10.3389/fmolb.2022.1108896 Text en Copyright © 2023 Caprari, Profumo, Massimi, Buttari, Riganò, Regine, Gabbianelli, Rossi, Risoluti, Materazzi, Gullifa, Maffei and Sorrentino. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Caprari, Patrizia
Profumo, Elisabetta
Massimi, Sara
Buttari, Brigitta
Riganò, Rachele
Regine, Vincenza
Gabbianelli, Marco
Rossi, Stefania
Risoluti, Roberta
Materazzi, Stefano
Gullifa, Giuseppina
Maffei, Laura
Sorrentino, Francesco
Hemorheological profiles and chronic inflammation markers in transfusion-dependent and non-transfusion- dependent thalassemia
title Hemorheological profiles and chronic inflammation markers in transfusion-dependent and non-transfusion- dependent thalassemia
title_full Hemorheological profiles and chronic inflammation markers in transfusion-dependent and non-transfusion- dependent thalassemia
title_fullStr Hemorheological profiles and chronic inflammation markers in transfusion-dependent and non-transfusion- dependent thalassemia
title_full_unstemmed Hemorheological profiles and chronic inflammation markers in transfusion-dependent and non-transfusion- dependent thalassemia
title_short Hemorheological profiles and chronic inflammation markers in transfusion-dependent and non-transfusion- dependent thalassemia
title_sort hemorheological profiles and chronic inflammation markers in transfusion-dependent and non-transfusion- dependent thalassemia
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868635/
https://www.ncbi.nlm.nih.gov/pubmed/36699704
http://dx.doi.org/10.3389/fmolb.2022.1108896
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