Placental Passage of Protopine in an Ex Vivo Human Perfusion System

The placental passage of protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated...

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Autores principales: Spiess, Deborah, Abegg, Vanessa Fabienne, Chauveau, Antoine, Treyer, Andrea, Reinehr, Michael, Oufir, Mouhssin, Duong, Elisa, Potterat, Olivier, Hamburger, Matthias, Simões-Wüst, Ana Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Georg Thieme Verlag KG 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868778/
https://www.ncbi.nlm.nih.gov/pubmed/35445384
http://dx.doi.org/10.1055/a-1829-9546
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author Spiess, Deborah
Abegg, Vanessa Fabienne
Chauveau, Antoine
Treyer, Andrea
Reinehr, Michael
Oufir, Mouhssin
Duong, Elisa
Potterat, Olivier
Hamburger, Matthias
Simões-Wüst, Ana Paula
author_facet Spiess, Deborah
Abegg, Vanessa Fabienne
Chauveau, Antoine
Treyer, Andrea
Reinehr, Michael
Oufir, Mouhssin
Duong, Elisa
Potterat, Olivier
Hamburger, Matthias
Simões-Wüst, Ana Paula
author_sort Spiess, Deborah
collection PubMed
description The placental passage of protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. The study compound did not affect placental viability or functionality, as glucose consumption, lactate production, and beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings.
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spelling pubmed-98687782023-01-24 Placental Passage of Protopine in an Ex Vivo Human Perfusion System Spiess, Deborah Abegg, Vanessa Fabienne Chauveau, Antoine Treyer, Andrea Reinehr, Michael Oufir, Mouhssin Duong, Elisa Potterat, Olivier Hamburger, Matthias Simões-Wüst, Ana Paula Planta Med The placental passage of protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. The study compound did not affect placental viability or functionality, as glucose consumption, lactate production, and beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings. Georg Thieme Verlag KG 2022-08-22 /pmc/articles/PMC9868778/ /pubmed/35445384 http://dx.doi.org/10.1055/a-1829-9546 Text en The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ ) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.
spellingShingle Spiess, Deborah
Abegg, Vanessa Fabienne
Chauveau, Antoine
Treyer, Andrea
Reinehr, Michael
Oufir, Mouhssin
Duong, Elisa
Potterat, Olivier
Hamburger, Matthias
Simões-Wüst, Ana Paula
Placental Passage of Protopine in an Ex Vivo Human Perfusion System
title Placental Passage of Protopine in an Ex Vivo Human Perfusion System
title_full Placental Passage of Protopine in an Ex Vivo Human Perfusion System
title_fullStr Placental Passage of Protopine in an Ex Vivo Human Perfusion System
title_full_unstemmed Placental Passage of Protopine in an Ex Vivo Human Perfusion System
title_short Placental Passage of Protopine in an Ex Vivo Human Perfusion System
title_sort placental passage of protopine in an ex vivo human perfusion system
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868778/
https://www.ncbi.nlm.nih.gov/pubmed/35445384
http://dx.doi.org/10.1055/a-1829-9546
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