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In-Vitro Growth Kinetics of Mesenchymal Stem Cells in Cytotoxicity Tests Using Low-Diluted Viscum Album

Introduction  The use of mesenchymal stem cells (MSC) in cytotoxicity tests is an in-vitro alternative model for predicting initial doses. Homeopathic medicines may stimulate the immune system to combat a pathology effectively and have been used for over two centuries. Viscum album (VA) extracts are...

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Autores principales: Valle, Ana Catarina Viana, Brunel, Hilana dos Santos Sena, Dallago, Bruno Stéfano Lima, Rodrigues, Lucas Santana, Malard, Patrícia Furtado, da Costa, Rosiane Andrade, Rossetto, Rafael, de Andrade, Rosângela Vieira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Georg Thieme Verlag KG 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868971/
https://www.ncbi.nlm.nih.gov/pubmed/35988582
http://dx.doi.org/10.1055/s-0042-1747682
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author Valle, Ana Catarina Viana
Brunel, Hilana dos Santos Sena
Dallago, Bruno Stéfano Lima
Rodrigues, Lucas Santana
Malard, Patrícia Furtado
da Costa, Rosiane Andrade
Rossetto, Rafael
de Andrade, Rosângela Vieira
author_facet Valle, Ana Catarina Viana
Brunel, Hilana dos Santos Sena
Dallago, Bruno Stéfano Lima
Rodrigues, Lucas Santana
Malard, Patrícia Furtado
da Costa, Rosiane Andrade
Rossetto, Rafael
de Andrade, Rosângela Vieira
author_sort Valle, Ana Catarina Viana
collection PubMed
description Introduction  The use of mesenchymal stem cells (MSC) in cytotoxicity tests is an in-vitro alternative model for predicting initial doses. Homeopathic medicines may stimulate the immune system to combat a pathology effectively and have been used for over two centuries. Viscum album (VA) extracts are widely used in the treatment of cancer, due to their immunomodulatory, cytotoxic and pro-apoptotic properties. Objective  This study aimed to evaluate the in-vitro growth kinetics of canine MSC in relation to cytotoxicity, cell differentiation and expression of pluripotentiality markers, using a VA preparation at the D1D2 (1×10 (−1) , 1×10 (−2) potency (VAD1D2). Methods  MSC were obtained from adipose tissue sampled from a healthy dog that was undergoing an elective veterinary procedure and with its owner's permission. The experiments were performed in three groups: MSC treated with VAD1D2 or diluent or untreated (control). The cytotoxicity was evaluated by MTT assay. The differentiation was induced in three lineages, and apoptotic cell labeling was performed by an Annexin-V test. Results  At the concentration of 10 μL/mL of VA, the number of cells after in-vitro culture was maintained when compared with the control (untreated) group. A significant and gradual decrease in cell viability was recorded as VA concentrations increased. The apoptosis analysis showed that VA at 20 μL/mL presented absolute percentages of initial apoptosis twice as high as at 10 μL/mL, which was similar to the control (untreated group). Conclusion  The results suggest that the use of efficient methods to assess the in-vitro cytotoxicity of VA-based homeopathic medicines using MSC lineages may predict the potential action at different concentrations. These findings demonstrated that VAD1D2 interferes with canine MSC growth kinetics.
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spelling pubmed-98689712023-01-24 In-Vitro Growth Kinetics of Mesenchymal Stem Cells in Cytotoxicity Tests Using Low-Diluted Viscum Album Valle, Ana Catarina Viana Brunel, Hilana dos Santos Sena Dallago, Bruno Stéfano Lima Rodrigues, Lucas Santana Malard, Patrícia Furtado da Costa, Rosiane Andrade Rossetto, Rafael de Andrade, Rosângela Vieira Homeopathy Introduction  The use of mesenchymal stem cells (MSC) in cytotoxicity tests is an in-vitro alternative model for predicting initial doses. Homeopathic medicines may stimulate the immune system to combat a pathology effectively and have been used for over two centuries. Viscum album (VA) extracts are widely used in the treatment of cancer, due to their immunomodulatory, cytotoxic and pro-apoptotic properties. Objective  This study aimed to evaluate the in-vitro growth kinetics of canine MSC in relation to cytotoxicity, cell differentiation and expression of pluripotentiality markers, using a VA preparation at the D1D2 (1×10 (−1) , 1×10 (−2) potency (VAD1D2). Methods  MSC were obtained from adipose tissue sampled from a healthy dog that was undergoing an elective veterinary procedure and with its owner's permission. The experiments were performed in three groups: MSC treated with VAD1D2 or diluent or untreated (control). The cytotoxicity was evaluated by MTT assay. The differentiation was induced in three lineages, and apoptotic cell labeling was performed by an Annexin-V test. Results  At the concentration of 10 μL/mL of VA, the number of cells after in-vitro culture was maintained when compared with the control (untreated) group. A significant and gradual decrease in cell viability was recorded as VA concentrations increased. The apoptosis analysis showed that VA at 20 μL/mL presented absolute percentages of initial apoptosis twice as high as at 10 μL/mL, which was similar to the control (untreated group). Conclusion  The results suggest that the use of efficient methods to assess the in-vitro cytotoxicity of VA-based homeopathic medicines using MSC lineages may predict the potential action at different concentrations. These findings demonstrated that VAD1D2 interferes with canine MSC growth kinetics. Georg Thieme Verlag KG 2022-08-21 /pmc/articles/PMC9868971/ /pubmed/35988582 http://dx.doi.org/10.1055/s-0042-1747682 Text en The Faculty of Homeopathy. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ ) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.
spellingShingle Valle, Ana Catarina Viana
Brunel, Hilana dos Santos Sena
Dallago, Bruno Stéfano Lima
Rodrigues, Lucas Santana
Malard, Patrícia Furtado
da Costa, Rosiane Andrade
Rossetto, Rafael
de Andrade, Rosângela Vieira
In-Vitro Growth Kinetics of Mesenchymal Stem Cells in Cytotoxicity Tests Using Low-Diluted Viscum Album
title In-Vitro Growth Kinetics of Mesenchymal Stem Cells in Cytotoxicity Tests Using Low-Diluted Viscum Album
title_full In-Vitro Growth Kinetics of Mesenchymal Stem Cells in Cytotoxicity Tests Using Low-Diluted Viscum Album
title_fullStr In-Vitro Growth Kinetics of Mesenchymal Stem Cells in Cytotoxicity Tests Using Low-Diluted Viscum Album
title_full_unstemmed In-Vitro Growth Kinetics of Mesenchymal Stem Cells in Cytotoxicity Tests Using Low-Diluted Viscum Album
title_short In-Vitro Growth Kinetics of Mesenchymal Stem Cells in Cytotoxicity Tests Using Low-Diluted Viscum Album
title_sort in-vitro growth kinetics of mesenchymal stem cells in cytotoxicity tests using low-diluted viscum album
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868971/
https://www.ncbi.nlm.nih.gov/pubmed/35988582
http://dx.doi.org/10.1055/s-0042-1747682
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