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Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet

A bacterial strain A1-3 with iprodione-degrading capabilities was isolated from the soil for vegetable growing under greenhouses at Lhasa, Tibet. Based on phenotypic, phylogenetic, and genotypic data, strain A1-3 was considered to represent a novel species of genus Azospirillum. It was able to use i...

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Autores principales: Pan, Hu, Zhu, Beike, Li, Jin, Zhou, Ziqiong, Bu, Wenbin, Dai, Yanna, Lu, Xiangyang, Liu, Huhu, Tian, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9869045/
https://www.ncbi.nlm.nih.gov/pubmed/36699606
http://dx.doi.org/10.3389/fmicb.2022.1057030
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author Pan, Hu
Zhu, Beike
Li, Jin
Zhou, Ziqiong
Bu, Wenbin
Dai, Yanna
Lu, Xiangyang
Liu, Huhu
Tian, Yun
author_facet Pan, Hu
Zhu, Beike
Li, Jin
Zhou, Ziqiong
Bu, Wenbin
Dai, Yanna
Lu, Xiangyang
Liu, Huhu
Tian, Yun
author_sort Pan, Hu
collection PubMed
description A bacterial strain A1-3 with iprodione-degrading capabilities was isolated from the soil for vegetable growing under greenhouses at Lhasa, Tibet. Based on phenotypic, phylogenetic, and genotypic data, strain A1-3 was considered to represent a novel species of genus Azospirillum. It was able to use iprodione as the sole source of carbon and energy for growth, 27.96 mg/L (50.80%) iprodione was reduced within 108 h at 25°C. During the degradation of iprodione by Azospirillum sp. A1-3, iprodione was firstly degraded to N-(3,5-dichlorophenyl)-2,4-dioxoimidazolidine, and then to (3,5-dichlorophenylurea) acetic acid. However, (3,5-dichlorophenylurea) acetic acid cannot be degraded to 3,5-dichloroaniline by Azospirillum sp. A1-3. A ipaH gene which has a highly similarity (98.72–99.92%) with other previously reported ipaH genes, was presented in Azospirillum sp. A1-3. Azospirillum novel strain with the ability of iprodione degradation associated with nitrogen fixation has never been reported to date, and Azospirillum sp. A1-3 might be a promising candidate for application in the bioremediation of iprodione-contaminated environments.
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spelling pubmed-98690452023-01-24 Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet Pan, Hu Zhu, Beike Li, Jin Zhou, Ziqiong Bu, Wenbin Dai, Yanna Lu, Xiangyang Liu, Huhu Tian, Yun Front Microbiol Microbiology A bacterial strain A1-3 with iprodione-degrading capabilities was isolated from the soil for vegetable growing under greenhouses at Lhasa, Tibet. Based on phenotypic, phylogenetic, and genotypic data, strain A1-3 was considered to represent a novel species of genus Azospirillum. It was able to use iprodione as the sole source of carbon and energy for growth, 27.96 mg/L (50.80%) iprodione was reduced within 108 h at 25°C. During the degradation of iprodione by Azospirillum sp. A1-3, iprodione was firstly degraded to N-(3,5-dichlorophenyl)-2,4-dioxoimidazolidine, and then to (3,5-dichlorophenylurea) acetic acid. However, (3,5-dichlorophenylurea) acetic acid cannot be degraded to 3,5-dichloroaniline by Azospirillum sp. A1-3. A ipaH gene which has a highly similarity (98.72–99.92%) with other previously reported ipaH genes, was presented in Azospirillum sp. A1-3. Azospirillum novel strain with the ability of iprodione degradation associated with nitrogen fixation has never been reported to date, and Azospirillum sp. A1-3 might be a promising candidate for application in the bioremediation of iprodione-contaminated environments. Frontiers Media S.A. 2023-01-09 /pmc/articles/PMC9869045/ /pubmed/36699606 http://dx.doi.org/10.3389/fmicb.2022.1057030 Text en Copyright © 2023 Pan, Zhu, Li, Zhou, Bu, Dai, Lu, Liu and Tian. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Pan, Hu
Zhu, Beike
Li, Jin
Zhou, Ziqiong
Bu, Wenbin
Dai, Yanna
Lu, Xiangyang
Liu, Huhu
Tian, Yun
Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet
title Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet
title_full Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet
title_fullStr Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet
title_full_unstemmed Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet
title_short Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet
title_sort degradation of iprodione by a novel strain azospirillum sp. a1-3 isolated from tibet
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9869045/
https://www.ncbi.nlm.nih.gov/pubmed/36699606
http://dx.doi.org/10.3389/fmicb.2022.1057030
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