Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations

Dilated cardiomyopathy (DCM) is a common heart disease that can lead to heart failure and sudden cardiac death. Mutations in the TTN gene are the most frequent cause of DCM. Here, we generated two human induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of...

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Autores principales: Zhang, Tina Tianbo, Zhao, Shane Rui, Alamana, Christina, Shen, Mengcheng, Parikh, Victoria, Wheeler, Matthew T., Wu, Joseph C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870071/
https://www.ncbi.nlm.nih.gov/pubmed/36270069
http://dx.doi.org/10.1016/j.scr.2022.102941
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author Zhang, Tina Tianbo
Zhao, Shane Rui
Alamana, Christina
Shen, Mengcheng
Parikh, Victoria
Wheeler, Matthew T.
Wu, Joseph C.
author_facet Zhang, Tina Tianbo
Zhao, Shane Rui
Alamana, Christina
Shen, Mengcheng
Parikh, Victoria
Wheeler, Matthew T.
Wu, Joseph C.
author_sort Zhang, Tina Tianbo
collection PubMed
description Dilated cardiomyopathy (DCM) is a common heart disease that can lead to heart failure and sudden cardiac death. Mutations in the TTN gene are the most frequent cause of DCM. Here, we generated two human induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of two DCM patients carrying c.94816C>T and c.104188A>G mutations in TTN, respectively. The two lines exhibited a normal morphology, full expression of pluripotency markers, a normal karyotype and the ability of trilineage differentiation. The two lines can serve as useful tools for drug screening and mechanism studies on DCM.
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spelling pubmed-98700712023-01-23 Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations Zhang, Tina Tianbo Zhao, Shane Rui Alamana, Christina Shen, Mengcheng Parikh, Victoria Wheeler, Matthew T. Wu, Joseph C. Stem Cell Res Article Dilated cardiomyopathy (DCM) is a common heart disease that can lead to heart failure and sudden cardiac death. Mutations in the TTN gene are the most frequent cause of DCM. Here, we generated two human induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of two DCM patients carrying c.94816C>T and c.104188A>G mutations in TTN, respectively. The two lines exhibited a normal morphology, full expression of pluripotency markers, a normal karyotype and the ability of trilineage differentiation. The two lines can serve as useful tools for drug screening and mechanism studies on DCM. 2022-12 2022-10-13 /pmc/articles/PMC9870071/ /pubmed/36270069 http://dx.doi.org/10.1016/j.scr.2022.102941 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Zhang, Tina Tianbo
Zhao, Shane Rui
Alamana, Christina
Shen, Mengcheng
Parikh, Victoria
Wheeler, Matthew T.
Wu, Joseph C.
Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations
title Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations
title_full Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations
title_fullStr Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations
title_full_unstemmed Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations
title_short Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations
title_sort generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying ttn mutations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870071/
https://www.ncbi.nlm.nih.gov/pubmed/36270069
http://dx.doi.org/10.1016/j.scr.2022.102941
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