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CRISPR/FnCas12a-mediated efficient multiplex and iterative genome editing in bacterial plant pathogens without donor DNA templates
CRISPR-based genome editing technology is revolutionizing prokaryotic research, but it has been rarely studied in bacterial plant pathogens. Here, we have developed a targeted genome editing method with no requirement of donor templates for convenient and efficient gene knockout in Xanthomonas oryza...
Autores principales: | Yan, Fang, Wang, Jingwen, Zhang, Sujie, Lu, Zhenwan, Li, Shaofang, Ji, Zhiyuan, Song, Congfeng, Chen, Gongyou, Xu, Jin, Feng, Jie, Zhou, Xueping, Zhou, Huanbin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870152/ https://www.ncbi.nlm.nih.gov/pubmed/36626407 http://dx.doi.org/10.1371/journal.ppat.1010961 |
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