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Electrophysiologically distinct bed nucleus of the stria terminalis projections to the ventral tegmental area in mice

The bed nucleus of the stria terminalis (BNST) is a highly heterogeneous limbic forebrain structure that serves as a relay connecting autonomic, neuroendocrine and behavioral function. It can be divided into over 16 individual subregions with distinct neuronal subpopulations based on receptors, tran...

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Autores principales: Miura, Yuka, Shanley, Mary Regis, Urbaez, Ashley, Friedman, Allyson K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870318/
https://www.ncbi.nlm.nih.gov/pubmed/36698552
http://dx.doi.org/10.3389/fncir.2022.1081099
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author Miura, Yuka
Shanley, Mary Regis
Urbaez, Ashley
Friedman, Allyson K.
author_facet Miura, Yuka
Shanley, Mary Regis
Urbaez, Ashley
Friedman, Allyson K.
author_sort Miura, Yuka
collection PubMed
description The bed nucleus of the stria terminalis (BNST) is a highly heterogeneous limbic forebrain structure that serves as a relay connecting autonomic, neuroendocrine and behavioral function. It can be divided into over 16 individual subregions with distinct neuronal subpopulations based on receptors, transmitters, and neuropeptides. Specifically, the BNST projection to the ventral tegmental area (VTA), the dopamine hub of the brain, has been shown to have a crucial role in the stress response. However, in mice there is a lack of unbiased data on the functional diversity of this sub-population which serves as an upstream input to the VTA. The dopaminergic neurons in the VTA modify their ion channel activity and intrinsic membrane properties to adapt to stress in part from inputs from BNST projections. Therefore, we aimed to perform a multi-component characterization of the functional diversity of the BNST-VTA pathway. We studied the passive and active electrophysiological properties of virally identified population of BNST neurons that project to the VTA. We used a comprehensive series of in vitro recordings of electrophysiological variables and performed hierarchical clustering to determine the functional diversity of the projection neurons in the BNST-VTA pathway. Our study revealed four subpopulations in the BNST-VTA pathway, all of which differ in their activation profiles and likely have distinct inputs and function in the VTA. Our results will help resolve the discord in interpretation of the various roles of this electrophysiologically diverse projection and builds a foundation for understanding how the different neuronal types integrate signals.
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spelling pubmed-98703182023-01-24 Electrophysiologically distinct bed nucleus of the stria terminalis projections to the ventral tegmental area in mice Miura, Yuka Shanley, Mary Regis Urbaez, Ashley Friedman, Allyson K. Front Neural Circuits Neuroscience The bed nucleus of the stria terminalis (BNST) is a highly heterogeneous limbic forebrain structure that serves as a relay connecting autonomic, neuroendocrine and behavioral function. It can be divided into over 16 individual subregions with distinct neuronal subpopulations based on receptors, transmitters, and neuropeptides. Specifically, the BNST projection to the ventral tegmental area (VTA), the dopamine hub of the brain, has been shown to have a crucial role in the stress response. However, in mice there is a lack of unbiased data on the functional diversity of this sub-population which serves as an upstream input to the VTA. The dopaminergic neurons in the VTA modify their ion channel activity and intrinsic membrane properties to adapt to stress in part from inputs from BNST projections. Therefore, we aimed to perform a multi-component characterization of the functional diversity of the BNST-VTA pathway. We studied the passive and active electrophysiological properties of virally identified population of BNST neurons that project to the VTA. We used a comprehensive series of in vitro recordings of electrophysiological variables and performed hierarchical clustering to determine the functional diversity of the projection neurons in the BNST-VTA pathway. Our study revealed four subpopulations in the BNST-VTA pathway, all of which differ in their activation profiles and likely have distinct inputs and function in the VTA. Our results will help resolve the discord in interpretation of the various roles of this electrophysiologically diverse projection and builds a foundation for understanding how the different neuronal types integrate signals. Frontiers Media S.A. 2023-01-09 /pmc/articles/PMC9870318/ /pubmed/36698552 http://dx.doi.org/10.3389/fncir.2022.1081099 Text en Copyright © 2023 Miura, Shanley, Urbaez and Friedman. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Miura, Yuka
Shanley, Mary Regis
Urbaez, Ashley
Friedman, Allyson K.
Electrophysiologically distinct bed nucleus of the stria terminalis projections to the ventral tegmental area in mice
title Electrophysiologically distinct bed nucleus of the stria terminalis projections to the ventral tegmental area in mice
title_full Electrophysiologically distinct bed nucleus of the stria terminalis projections to the ventral tegmental area in mice
title_fullStr Electrophysiologically distinct bed nucleus of the stria terminalis projections to the ventral tegmental area in mice
title_full_unstemmed Electrophysiologically distinct bed nucleus of the stria terminalis projections to the ventral tegmental area in mice
title_short Electrophysiologically distinct bed nucleus of the stria terminalis projections to the ventral tegmental area in mice
title_sort electrophysiologically distinct bed nucleus of the stria terminalis projections to the ventral tegmental area in mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870318/
https://www.ncbi.nlm.nih.gov/pubmed/36698552
http://dx.doi.org/10.3389/fncir.2022.1081099
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