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Mucus-derived exosome-like vesicles from the Spanish slug (Arion vulgaris): taking advantage of invasive pest species in biotechnology

The slug Arion vulgaris has attracted major attention as one of the worst invasive herbivore pests in Europe and is renowned for the stiff mucus it secretes for locomotion. In this study we focused on the isolation and characterisation of extracellular vesicles, specifically exosomes and exosome-lik...

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Autores principales: Liegertová, Michaela, Semerádtová, Alena, Kocholatá, Michaela, Průšová, Michaela, Němcová, Lenka, Štofik, Marcel, Kříženecká, Sylvie, Malý, Jan, Janoušková, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870906/
https://www.ncbi.nlm.nih.gov/pubmed/36526668
http://dx.doi.org/10.1038/s41598-022-26335-3
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author Liegertová, Michaela
Semerádtová, Alena
Kocholatá, Michaela
Průšová, Michaela
Němcová, Lenka
Štofik, Marcel
Kříženecká, Sylvie
Malý, Jan
Janoušková, Olga
author_facet Liegertová, Michaela
Semerádtová, Alena
Kocholatá, Michaela
Průšová, Michaela
Němcová, Lenka
Štofik, Marcel
Kříženecká, Sylvie
Malý, Jan
Janoušková, Olga
author_sort Liegertová, Michaela
collection PubMed
description The slug Arion vulgaris has attracted major attention as one of the worst invasive herbivore pests in Europe and is renowned for the stiff mucus it secretes for locomotion. In this study we focused on the isolation and characterisation of extracellular vesicles, specifically exosomes and exosome-like vesicles, from Arion secretions. We developed a method for slug mucus collection and subsequent vesicle isolation by ultracentrifugation. The isolated vesicles with an average diameter of ~ 100 nm carry abundant proteins and short RNAs, as well as adhesion molecules similar to mammalian galectins. We demonstrated that the slug extracellular vesicles are internalised by plant cells and human cancer cells in in vitro assays and are loadable by bioactive compounds, which makes them an interesting tool for utilisation in biotechnology.
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spelling pubmed-98709062023-01-25 Mucus-derived exosome-like vesicles from the Spanish slug (Arion vulgaris): taking advantage of invasive pest species in biotechnology Liegertová, Michaela Semerádtová, Alena Kocholatá, Michaela Průšová, Michaela Němcová, Lenka Štofik, Marcel Kříženecká, Sylvie Malý, Jan Janoušková, Olga Sci Rep Article The slug Arion vulgaris has attracted major attention as one of the worst invasive herbivore pests in Europe and is renowned for the stiff mucus it secretes for locomotion. In this study we focused on the isolation and characterisation of extracellular vesicles, specifically exosomes and exosome-like vesicles, from Arion secretions. We developed a method for slug mucus collection and subsequent vesicle isolation by ultracentrifugation. The isolated vesicles with an average diameter of ~ 100 nm carry abundant proteins and short RNAs, as well as adhesion molecules similar to mammalian galectins. We demonstrated that the slug extracellular vesicles are internalised by plant cells and human cancer cells in in vitro assays and are loadable by bioactive compounds, which makes them an interesting tool for utilisation in biotechnology. Nature Publishing Group UK 2022-12-16 /pmc/articles/PMC9870906/ /pubmed/36526668 http://dx.doi.org/10.1038/s41598-022-26335-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Liegertová, Michaela
Semerádtová, Alena
Kocholatá, Michaela
Průšová, Michaela
Němcová, Lenka
Štofik, Marcel
Kříženecká, Sylvie
Malý, Jan
Janoušková, Olga
Mucus-derived exosome-like vesicles from the Spanish slug (Arion vulgaris): taking advantage of invasive pest species in biotechnology
title Mucus-derived exosome-like vesicles from the Spanish slug (Arion vulgaris): taking advantage of invasive pest species in biotechnology
title_full Mucus-derived exosome-like vesicles from the Spanish slug (Arion vulgaris): taking advantage of invasive pest species in biotechnology
title_fullStr Mucus-derived exosome-like vesicles from the Spanish slug (Arion vulgaris): taking advantage of invasive pest species in biotechnology
title_full_unstemmed Mucus-derived exosome-like vesicles from the Spanish slug (Arion vulgaris): taking advantage of invasive pest species in biotechnology
title_short Mucus-derived exosome-like vesicles from the Spanish slug (Arion vulgaris): taking advantage of invasive pest species in biotechnology
title_sort mucus-derived exosome-like vesicles from the spanish slug (arion vulgaris): taking advantage of invasive pest species in biotechnology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870906/
https://www.ncbi.nlm.nih.gov/pubmed/36526668
http://dx.doi.org/10.1038/s41598-022-26335-3
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