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Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell

Formation of electrogenic microbial biofilm on the electrode is critical for harvesting electrical power from wastewater in microbial biofuel cells (MFCs). Although the knowledge of bacterial community structures in the biofilm is vital for the rational design of MFC electrodes, an in-depth study on...

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Autores principales: Hemdan, Bahaa A., El-Taweel, Gamila E., Naha, Sunandan, Goswami, Pranab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871009/
https://www.ncbi.nlm.nih.gov/pubmed/36690637
http://dx.doi.org/10.1038/s41598-023-27795-x
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author Hemdan, Bahaa A.
El-Taweel, Gamila E.
Naha, Sunandan
Goswami, Pranab
author_facet Hemdan, Bahaa A.
El-Taweel, Gamila E.
Naha, Sunandan
Goswami, Pranab
author_sort Hemdan, Bahaa A.
collection PubMed
description Formation of electrogenic microbial biofilm on the electrode is critical for harvesting electrical power from wastewater in microbial biofuel cells (MFCs). Although the knowledge of bacterial community structures in the biofilm is vital for the rational design of MFC electrodes, an in-depth study on the subject is still awaiting. Herein, we attempt to address this issue by creating electrogenic biofilm on modified graphite anodes assembled in an air–cathode MFC. The modification was performed with reduced graphene oxide (rGO), polyaniline (PANI), and carbon nanotube (CNTs) separately. To accelerate the growth of the biofilm, soybean-potato composite (plant) powder was blended with these conductive materials during the fabrication of the anodes. The MFC fabricated with PANI-based anode delivered the current density of 324.2 mA cm(−2), followed by CNTs (248.75 mA cm(−2)), rGO (193 mA cm(−2)), and blank (without coating) (151 mA cm(−2)) graphite electrodes. Likewise, the PANI-based anode supported a robust biofilm growth containing maximum bacterial cell densities with diverse shapes and sizes of the cells and broad metabolic functionality. The alpha diversity of the biofilm developed over the anode coated with PANI was the loftiest operational taxonomic unit (2058 OUT) and Shannon index (7.56), as disclosed from the high-throughput 16S rRNA sequence analysis. Further, within these taxonomic units, exoelectrogenic phyla comprising Proteobacteria, Firmicutes, and Bacteroidetes were maximum with their corresponding level (%) 45.5, 36.2, and 9.8. The relative abundance of Gammaproteobacteria, Clostridia, and Bacilli at the class level, while Pseudomonas, Clostridium, Enterococcus, and Bifidobacterium at the genus level were comparatively higher in the PANI-based anode.
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spelling pubmed-98710092023-01-25 Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell Hemdan, Bahaa A. El-Taweel, Gamila E. Naha, Sunandan Goswami, Pranab Sci Rep Article Formation of electrogenic microbial biofilm on the electrode is critical for harvesting electrical power from wastewater in microbial biofuel cells (MFCs). Although the knowledge of bacterial community structures in the biofilm is vital for the rational design of MFC electrodes, an in-depth study on the subject is still awaiting. Herein, we attempt to address this issue by creating electrogenic biofilm on modified graphite anodes assembled in an air–cathode MFC. The modification was performed with reduced graphene oxide (rGO), polyaniline (PANI), and carbon nanotube (CNTs) separately. To accelerate the growth of the biofilm, soybean-potato composite (plant) powder was blended with these conductive materials during the fabrication of the anodes. The MFC fabricated with PANI-based anode delivered the current density of 324.2 mA cm(−2), followed by CNTs (248.75 mA cm(−2)), rGO (193 mA cm(−2)), and blank (without coating) (151 mA cm(−2)) graphite electrodes. Likewise, the PANI-based anode supported a robust biofilm growth containing maximum bacterial cell densities with diverse shapes and sizes of the cells and broad metabolic functionality. The alpha diversity of the biofilm developed over the anode coated with PANI was the loftiest operational taxonomic unit (2058 OUT) and Shannon index (7.56), as disclosed from the high-throughput 16S rRNA sequence analysis. Further, within these taxonomic units, exoelectrogenic phyla comprising Proteobacteria, Firmicutes, and Bacteroidetes were maximum with their corresponding level (%) 45.5, 36.2, and 9.8. The relative abundance of Gammaproteobacteria, Clostridia, and Bacilli at the class level, while Pseudomonas, Clostridium, Enterococcus, and Bifidobacterium at the genus level were comparatively higher in the PANI-based anode. Nature Publishing Group UK 2023-01-23 /pmc/articles/PMC9871009/ /pubmed/36690637 http://dx.doi.org/10.1038/s41598-023-27795-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hemdan, Bahaa A.
El-Taweel, Gamila E.
Naha, Sunandan
Goswami, Pranab
Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell
title Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell
title_full Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell
title_fullStr Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell
title_full_unstemmed Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell
title_short Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell
title_sort bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871009/
https://www.ncbi.nlm.nih.gov/pubmed/36690637
http://dx.doi.org/10.1038/s41598-023-27795-x
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