Protocol to access unknown flanking DNA sequences using Wristwatch-PCR for genome-walking

Here we describe a protocol for wristwatch PCR, an approach based on wristwatch-like structure formed between walking primers to obtain unknown flanks. We specify the criteria for designing wristwatch primers and gene-specific primers. We detail how to set wristwatch primer permutations to obtain pe...

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Detalles Bibliográficos
Autores principales: Wang, Lingqin, Jia, Mengya, Li, Zhaoqin, Liu, Xiaohua, Sun, Tianyi, Pei, Jinfeng, Wei, Cheng, Lin, Zhiyu, Li, Haixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871321/
https://www.ncbi.nlm.nih.gov/pubmed/36853735
http://dx.doi.org/10.1016/j.xpro.2022.102037

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