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Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon

Bacterial membrane vesicles have emerged as gadgets allowing remote communication between the microbiota and distal host organs. Here we describe a protocol for enriching vesicles from serum and colon that could widely be adapted for other tissues. We detail pre-clearing of serum or colon fluids usi...

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Detalles Bibliográficos
Autores principales: Erttmann, Saskia F., Gekara, Nelson O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871346/
https://www.ncbi.nlm.nih.gov/pubmed/36853709
http://dx.doi.org/10.1016/j.xpro.2023.102046