Production of CRISPRi-engineered primary human mammary epithelial cells with baboon envelope pseudotyped lentiviral vectors

Primary human mammary epithelial cells (pHMECs) are known to be remarkably difficult to engineer genetically. Here, we present a protocol for efficient transduction of pHMECs using a baboon retroviral envelope glycoprotein for pseudotyping of lentiviral vectors (BaEV-LVs). We describe the preparatio...

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Detalles Bibliográficos
Autores principales: Pastor, Sonia, Wicinski, Julien, Charafe-Jauffret, Emmanuelle, Verhoeyen, Els, Guittard, Geoffrey, Ginestier, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871349/
https://www.ncbi.nlm.nih.gov/pubmed/36853718
http://dx.doi.org/10.1016/j.xpro.2023.102055

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871349/
https://www.ncbi.nlm.nih.gov/pubmed/36853718
http://dx.doi.org/10.1016/j.xpro.2023.102055

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