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Acute and subacute oral toxicity assessment of Gancao Xiexin decoction in Sprague-Dawley rats

Gancao Xiexin decoction (GCXXD), a well-known classic traditional Chinese medicine prescription, is used to treat various oral ulcers, Behcet disease, gastrointestinal ulcers, etc. However, there is very little information on its safety. This study aimed to investigate the acute and subacute oral to...

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Autores principales: Ru, Li, Liu, Ruotong, Xing, Haoyu, Yuan, Yueming, Yuan, Zheng, Xu, Zhiyong, Xu, Qin, Song, Jianping, Li, Xiaobo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871357/
https://www.ncbi.nlm.nih.gov/pubmed/36703742
http://dx.doi.org/10.3389/fphar.2022.1078665
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author Ru, Li
Liu, Ruotong
Xing, Haoyu
Yuan, Yueming
Yuan, Zheng
Xu, Zhiyong
Xu, Qin
Song, Jianping
Li, Xiaobo
author_facet Ru, Li
Liu, Ruotong
Xing, Haoyu
Yuan, Yueming
Yuan, Zheng
Xu, Zhiyong
Xu, Qin
Song, Jianping
Li, Xiaobo
author_sort Ru, Li
collection PubMed
description Gancao Xiexin decoction (GCXXD), a well-known classic traditional Chinese medicine prescription, is used to treat various oral ulcers, Behcet disease, gastrointestinal ulcers, etc. However, there is very little information on its safety. This study aimed to investigate the acute and subacute oral toxicity of GCXXD in Sprague-Dawley rats. In the acute toxicity study, rats were orally administered 10 g/kg GCXXD three times a day. Clinical signs of abnormality and mortality were observed daily for 14 days. In the subacute toxicity study, rats were orally administered 0, 1.47, 3.83, or 10 g/kg GCXXD for 28 days. The rats’ clinical signs, body weight, food consumption, hematological and biochemical parameters, bone marrow smear, organ index, and pathological morphology were analyzed. The acute toxicity study showed that GCXXD is safe in rats without any obvious toxicity via an oral dose of 30 g/kg/day (3 × 10 g/kg). After 28 days of administration, slightly decreased RBC, HGB, and HCT were observed in female rats at 10 g/kg, suggesting that repeated doses of high-dose GCXXD may cause mild anemia in female rats. The no-observed-adverse-effect level (NOAEL) and lowest-observed-adverse-effect level (LOAEL) of oral administration of GCXXD for 28 days in rats are considered to be 3.83 g/kg and 10 g/kg, respectively. Long-term toxicity studies are recommended to strengthen the findings.
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spelling pubmed-98713572023-01-25 Acute and subacute oral toxicity assessment of Gancao Xiexin decoction in Sprague-Dawley rats Ru, Li Liu, Ruotong Xing, Haoyu Yuan, Yueming Yuan, Zheng Xu, Zhiyong Xu, Qin Song, Jianping Li, Xiaobo Front Pharmacol Pharmacology Gancao Xiexin decoction (GCXXD), a well-known classic traditional Chinese medicine prescription, is used to treat various oral ulcers, Behcet disease, gastrointestinal ulcers, etc. However, there is very little information on its safety. This study aimed to investigate the acute and subacute oral toxicity of GCXXD in Sprague-Dawley rats. In the acute toxicity study, rats were orally administered 10 g/kg GCXXD three times a day. Clinical signs of abnormality and mortality were observed daily for 14 days. In the subacute toxicity study, rats were orally administered 0, 1.47, 3.83, or 10 g/kg GCXXD for 28 days. The rats’ clinical signs, body weight, food consumption, hematological and biochemical parameters, bone marrow smear, organ index, and pathological morphology were analyzed. The acute toxicity study showed that GCXXD is safe in rats without any obvious toxicity via an oral dose of 30 g/kg/day (3 × 10 g/kg). After 28 days of administration, slightly decreased RBC, HGB, and HCT were observed in female rats at 10 g/kg, suggesting that repeated doses of high-dose GCXXD may cause mild anemia in female rats. The no-observed-adverse-effect level (NOAEL) and lowest-observed-adverse-effect level (LOAEL) of oral administration of GCXXD for 28 days in rats are considered to be 3.83 g/kg and 10 g/kg, respectively. Long-term toxicity studies are recommended to strengthen the findings. Frontiers Media S.A. 2023-01-10 /pmc/articles/PMC9871357/ /pubmed/36703742 http://dx.doi.org/10.3389/fphar.2022.1078665 Text en Copyright © 2023 Ru, Liu, Xing, Yuan, Yuan, Xu, Xu, Song and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Ru, Li
Liu, Ruotong
Xing, Haoyu
Yuan, Yueming
Yuan, Zheng
Xu, Zhiyong
Xu, Qin
Song, Jianping
Li, Xiaobo
Acute and subacute oral toxicity assessment of Gancao Xiexin decoction in Sprague-Dawley rats
title Acute and subacute oral toxicity assessment of Gancao Xiexin decoction in Sprague-Dawley rats
title_full Acute and subacute oral toxicity assessment of Gancao Xiexin decoction in Sprague-Dawley rats
title_fullStr Acute and subacute oral toxicity assessment of Gancao Xiexin decoction in Sprague-Dawley rats
title_full_unstemmed Acute and subacute oral toxicity assessment of Gancao Xiexin decoction in Sprague-Dawley rats
title_short Acute and subacute oral toxicity assessment of Gancao Xiexin decoction in Sprague-Dawley rats
title_sort acute and subacute oral toxicity assessment of gancao xiexin decoction in sprague-dawley rats
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871357/
https://www.ncbi.nlm.nih.gov/pubmed/36703742
http://dx.doi.org/10.3389/fphar.2022.1078665
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