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A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants

The emergence of severe acute respiratory syndrome coronavirus type II (SARS-CoV-2) variants have led to a decline in the protection of existing vaccines and antibodies, and there is an urgent need for a broad-spectrum vaccination strategy to reduce the pressure on the prevention and control of the...

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Autores principales: Xu, Huifang, Wang, Tiantian, Sun, Peng, Hou, Xuchen, Gong, Xin, Zhang, Bin, Wu, Jun, Liu, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871450/
https://www.ncbi.nlm.nih.gov/pubmed/36704561
http://dx.doi.org/10.3389/fmicb.2022.1093080
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author Xu, Huifang
Wang, Tiantian
Sun, Peng
Hou, Xuchen
Gong, Xin
Zhang, Bin
Wu, Jun
Liu, Bo
author_facet Xu, Huifang
Wang, Tiantian
Sun, Peng
Hou, Xuchen
Gong, Xin
Zhang, Bin
Wu, Jun
Liu, Bo
author_sort Xu, Huifang
collection PubMed
description The emergence of severe acute respiratory syndrome coronavirus type II (SARS-CoV-2) variants have led to a decline in the protection of existing vaccines and antibodies, and there is an urgent need for a broad-spectrum vaccination strategy to reduce the pressure on the prevention and control of the pandemic. In this study, the receptor binding domain (RBD) of the SARS-CoV-2 Beta variant was successfully expressed through a glycoengineered yeast platform. To pursue a more broad-spectrum vaccination strategy, RBD-Beta and RBD-wild type were mixed at the ratio of 1:1 with Al(OH)(3) and CpG double adjuvants for the immunization of BALB/c mice. This bivalent vaccine stimulated robust conjugated antibody titers and a broader spectrum of neutralizing antibody titers. These results suggested that a bivalent vaccine of RBD-Beta and RBD-wild type could be a possible broad-spectrum vaccination strategy.
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spelling pubmed-98714502023-01-25 A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants Xu, Huifang Wang, Tiantian Sun, Peng Hou, Xuchen Gong, Xin Zhang, Bin Wu, Jun Liu, Bo Front Microbiol Microbiology The emergence of severe acute respiratory syndrome coronavirus type II (SARS-CoV-2) variants have led to a decline in the protection of existing vaccines and antibodies, and there is an urgent need for a broad-spectrum vaccination strategy to reduce the pressure on the prevention and control of the pandemic. In this study, the receptor binding domain (RBD) of the SARS-CoV-2 Beta variant was successfully expressed through a glycoengineered yeast platform. To pursue a more broad-spectrum vaccination strategy, RBD-Beta and RBD-wild type were mixed at the ratio of 1:1 with Al(OH)(3) and CpG double adjuvants for the immunization of BALB/c mice. This bivalent vaccine stimulated robust conjugated antibody titers and a broader spectrum of neutralizing antibody titers. These results suggested that a bivalent vaccine of RBD-Beta and RBD-wild type could be a possible broad-spectrum vaccination strategy. Frontiers Media S.A. 2023-01-10 /pmc/articles/PMC9871450/ /pubmed/36704561 http://dx.doi.org/10.3389/fmicb.2022.1093080 Text en Copyright © 2023 Xu, Wang, Sun, Hou, Gong, Zhang, Wu and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xu, Huifang
Wang, Tiantian
Sun, Peng
Hou, Xuchen
Gong, Xin
Zhang, Bin
Wu, Jun
Liu, Bo
A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants
title A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants
title_full A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants
title_fullStr A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants
title_full_unstemmed A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants
title_short A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants
title_sort bivalent subunit vaccine efficiently produced in pichia pastoris against sars-cov-2 and emerging variants
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871450/
https://www.ncbi.nlm.nih.gov/pubmed/36704561
http://dx.doi.org/10.3389/fmicb.2022.1093080
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