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Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione

Fungal pathogen contamination is one of the most important factors affecting the postharvest quality and shelf life of wolfberry fruits. Therefore, the prevention and control of fungal pathogens that cause fruit rot has become particularly important. Volatile antifungal agents of biological origin h...

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Detalles Bibliográficos
Autores principales: Ling, Lijun, Luo, Hong, Zhao, Yunhua, Yang, Caiyun, Cheng, Wenting, Pang, Mingmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871540/
https://www.ncbi.nlm.nih.gov/pubmed/36704548
http://dx.doi.org/10.3389/fmicb.2022.1068144
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author Ling, Lijun
Luo, Hong
Zhao, Yunhua
Yang, Caiyun
Cheng, Wenting
Pang, Mingmei
author_facet Ling, Lijun
Luo, Hong
Zhao, Yunhua
Yang, Caiyun
Cheng, Wenting
Pang, Mingmei
author_sort Ling, Lijun
collection PubMed
description Fungal pathogen contamination is one of the most important factors affecting the postharvest quality and shelf life of wolfberry fruits. Therefore, the prevention and control of fungal pathogens that cause fruit rot has become particularly important. Volatile antifungal agents of biological origin have broad application prospects. They may be safer and more efficient than traditional physical and chemical methods. Four pathogenic fungi were isolated and purified from rotting wolfberry. These pathogenic fungi were determined to be Mucor circinelloides LB1, Fusarium arcuatisporum LB5, Alternaria iridiaustralis LB7, and Colletotrichum fioriniae LB8. In vitro fumigation experiments showed that 2,3-butanedione can effectively inhibit the mycelial growth, spore germination, and sporulation ability of pathogenic fungi. The scanning electron microscope (SEM) showed morphological changes in hyphae. Propidium iodide (PI) Staining and leakage of 260 and 280 nm-absorbing increased, suggesting damage to cell membranes. Furthermore, 2,3-butanedione was found to significantly improve fruit firmness, soluble solid, total phenol, flavonoid, and soluble sugar content, as well as higher SOD enzyme activity and lower PPO and POD enzyme activity in the treated fruit, indicating that 2,3-butanedione can effectively reduce the adverse effects of pathogenic fungi in wolfberry. Based on these results, we conclude that 2,3-butanedione is effective against infection by pathogenic fungi in post-harvest wolfberry. 2,3-butanedione should be considered a viable substitute for conventional fungicides that are currently used to control rot in wolfberry.
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spelling pubmed-98715402023-01-25 Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione Ling, Lijun Luo, Hong Zhao, Yunhua Yang, Caiyun Cheng, Wenting Pang, Mingmei Front Microbiol Microbiology Fungal pathogen contamination is one of the most important factors affecting the postharvest quality and shelf life of wolfberry fruits. Therefore, the prevention and control of fungal pathogens that cause fruit rot has become particularly important. Volatile antifungal agents of biological origin have broad application prospects. They may be safer and more efficient than traditional physical and chemical methods. Four pathogenic fungi were isolated and purified from rotting wolfberry. These pathogenic fungi were determined to be Mucor circinelloides LB1, Fusarium arcuatisporum LB5, Alternaria iridiaustralis LB7, and Colletotrichum fioriniae LB8. In vitro fumigation experiments showed that 2,3-butanedione can effectively inhibit the mycelial growth, spore germination, and sporulation ability of pathogenic fungi. The scanning electron microscope (SEM) showed morphological changes in hyphae. Propidium iodide (PI) Staining and leakage of 260 and 280 nm-absorbing increased, suggesting damage to cell membranes. Furthermore, 2,3-butanedione was found to significantly improve fruit firmness, soluble solid, total phenol, flavonoid, and soluble sugar content, as well as higher SOD enzyme activity and lower PPO and POD enzyme activity in the treated fruit, indicating that 2,3-butanedione can effectively reduce the adverse effects of pathogenic fungi in wolfberry. Based on these results, we conclude that 2,3-butanedione is effective against infection by pathogenic fungi in post-harvest wolfberry. 2,3-butanedione should be considered a viable substitute for conventional fungicides that are currently used to control rot in wolfberry. Frontiers Media S.A. 2023-01-10 /pmc/articles/PMC9871540/ /pubmed/36704548 http://dx.doi.org/10.3389/fmicb.2022.1068144 Text en Copyright © 2023 Ling, Luo, Zhao, Yang, Cheng and Pang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ling, Lijun
Luo, Hong
Zhao, Yunhua
Yang, Caiyun
Cheng, Wenting
Pang, Mingmei
Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione
title Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione
title_full Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione
title_fullStr Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione
title_full_unstemmed Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione
title_short Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione
title_sort fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871540/
https://www.ncbi.nlm.nih.gov/pubmed/36704548
http://dx.doi.org/10.3389/fmicb.2022.1068144
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