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Moist exposed burn ointment accelerates diabetes-related wound healing by promoting re-epithelialization

BACKGROUND: The incidence of diabetes-related wounds is widespread, and the treatment is challenging. We found that Moist Exposed Burn Ointment (MEBO) promotes the healing of diabetes-related wounds, but the mechanism is not clear. METHODS: This study aimed to explore the mechanism of MEBO on diabet...

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Autores principales: Gong, Yuanxun, Jiang, Yan, Huang, Jinmei, He, Zuofen, Tang, Qianli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871640/
https://www.ncbi.nlm.nih.gov/pubmed/36703885
http://dx.doi.org/10.3389/fmed.2022.1042015
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author Gong, Yuanxun
Jiang, Yan
Huang, Jinmei
He, Zuofen
Tang, Qianli
author_facet Gong, Yuanxun
Jiang, Yan
Huang, Jinmei
He, Zuofen
Tang, Qianli
author_sort Gong, Yuanxun
collection PubMed
description BACKGROUND: The incidence of diabetes-related wounds is widespread, and the treatment is challenging. We found that Moist Exposed Burn Ointment (MEBO) promotes the healing of diabetes-related wounds, but the mechanism is not clear. METHODS: This study aimed to explore the mechanism of MEBO on diabetic wound healing, which may be related to the promotion of re-epithelialization. A full-thickness skin resection model was established in streptozotocin (STZ)-induced diabetic mice. MEBO and Kangfuxin (KFX) were applied to the wound area, and the wound healing rate was analyzed by photographing. The granulation tissue and epidermal thickness, the collagen remodeling rate, and the expression of cytokeratin 10 (CK10), cytokeratin 14 (CK14), Ki67, Collagen I, and Collagen III in the regenerated skin were detected by H&E staining, Masson staining, and immunofluorescence staining, respectively. MEBO and KFX were applied to human immortalized keratinocytes (HaCaT), mouse dermal fibrolasts (MDF) cells, and cell viability, cell migration, and differentiation were determined by CCK-8, scratching assay, RT-qPCR, and Western blot (WB), respectively. RESULTS: We found that MEBO significantly promoted the formation of wound granulation tissue and collagen remodeling in diabetic mice. The application of MEBO to diabetic wounds not only promoted the formation of hair follicles and sebaceous glands but also promoted the expression of Ki67, CK10, and CK14 in epidermal cells. MEBO had no significant effect on the differentiation process of keratinocytes. CONCLUSION: Our study further proved that MEBO plays a positive role in diabetic wound healing, and its excellent ability to promote re-epithelialization may be an important reason for promoting wound healing.
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spelling pubmed-98716402023-01-25 Moist exposed burn ointment accelerates diabetes-related wound healing by promoting re-epithelialization Gong, Yuanxun Jiang, Yan Huang, Jinmei He, Zuofen Tang, Qianli Front Med (Lausanne) Medicine BACKGROUND: The incidence of diabetes-related wounds is widespread, and the treatment is challenging. We found that Moist Exposed Burn Ointment (MEBO) promotes the healing of diabetes-related wounds, but the mechanism is not clear. METHODS: This study aimed to explore the mechanism of MEBO on diabetic wound healing, which may be related to the promotion of re-epithelialization. A full-thickness skin resection model was established in streptozotocin (STZ)-induced diabetic mice. MEBO and Kangfuxin (KFX) were applied to the wound area, and the wound healing rate was analyzed by photographing. The granulation tissue and epidermal thickness, the collagen remodeling rate, and the expression of cytokeratin 10 (CK10), cytokeratin 14 (CK14), Ki67, Collagen I, and Collagen III in the regenerated skin were detected by H&E staining, Masson staining, and immunofluorescence staining, respectively. MEBO and KFX were applied to human immortalized keratinocytes (HaCaT), mouse dermal fibrolasts (MDF) cells, and cell viability, cell migration, and differentiation were determined by CCK-8, scratching assay, RT-qPCR, and Western blot (WB), respectively. RESULTS: We found that MEBO significantly promoted the formation of wound granulation tissue and collagen remodeling in diabetic mice. The application of MEBO to diabetic wounds not only promoted the formation of hair follicles and sebaceous glands but also promoted the expression of Ki67, CK10, and CK14 in epidermal cells. MEBO had no significant effect on the differentiation process of keratinocytes. CONCLUSION: Our study further proved that MEBO plays a positive role in diabetic wound healing, and its excellent ability to promote re-epithelialization may be an important reason for promoting wound healing. Frontiers Media S.A. 2023-01-10 /pmc/articles/PMC9871640/ /pubmed/36703885 http://dx.doi.org/10.3389/fmed.2022.1042015 Text en Copyright © 2023 Gong, Jiang, Huang, He and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Gong, Yuanxun
Jiang, Yan
Huang, Jinmei
He, Zuofen
Tang, Qianli
Moist exposed burn ointment accelerates diabetes-related wound healing by promoting re-epithelialization
title Moist exposed burn ointment accelerates diabetes-related wound healing by promoting re-epithelialization
title_full Moist exposed burn ointment accelerates diabetes-related wound healing by promoting re-epithelialization
title_fullStr Moist exposed burn ointment accelerates diabetes-related wound healing by promoting re-epithelialization
title_full_unstemmed Moist exposed burn ointment accelerates diabetes-related wound healing by promoting re-epithelialization
title_short Moist exposed burn ointment accelerates diabetes-related wound healing by promoting re-epithelialization
title_sort moist exposed burn ointment accelerates diabetes-related wound healing by promoting re-epithelialization
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871640/
https://www.ncbi.nlm.nih.gov/pubmed/36703885
http://dx.doi.org/10.3389/fmed.2022.1042015
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AT hezuofen moistexposedburnointmentacceleratesdiabetesrelatedwoundhealingbypromotingreepithelialization
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