Large-scale sampling of C. elegans on E. coli HT115 RNAi-compatible bacteria

As opposed to standard culturing, growing large numbers of C. elegans worms on Nematode Growth Medium-based RNA interference (RNAi) plates requires multiple transferring steps to prevent starvation, which increase handling time and reduce practical efficiency. We here provide an optimized method to...

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Detalles Bibliográficos
Autores principales: Boelen, Rose, Temmerman, Liesbet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871793/
https://www.ncbi.nlm.nih.gov/pubmed/36703717
http://dx.doi.org/10.17912/micropub.biology.000692
Descripción
Sumario:As opposed to standard culturing, growing large numbers of C. elegans worms on Nematode Growth Medium-based RNA interference (RNAi) plates requires multiple transferring steps to prevent starvation, which increase handling time and reduce practical efficiency. We here provide an optimized method to grow four times more worms in RNAi conditions, thus saving on required resources and handling steps.

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