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Large-scale sampling of C. elegans on E. coli HT115 RNAi-compatible bacteria
As opposed to standard culturing, growing large numbers of C. elegans worms on Nematode Growth Medium-based RNA interference (RNAi) plates requires multiple transferring steps to prevent starvation, which increase handling time and reduce practical efficiency. We here provide an optimized method to...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Caltech Library
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871793/ https://www.ncbi.nlm.nih.gov/pubmed/36703717 http://dx.doi.org/10.17912/micropub.biology.000692 |
| _version_ | 1784877259982635008 |
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| author | Boelen, Rose Temmerman, Liesbet |
| author_facet | Boelen, Rose Temmerman, Liesbet |
| author_sort | Boelen, Rose |
| collection | PubMed |
| description | As opposed to standard culturing, growing large numbers of C. elegans worms on Nematode Growth Medium-based RNA interference (RNAi) plates requires multiple transferring steps to prevent starvation, which increase handling time and reduce practical efficiency. We here provide an optimized method to grow four times more worms in RNAi conditions, thus saving on required resources and handling steps. |
| format | Online Article Text |
| id | pubmed-9871793 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Caltech Library |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-98717932023-01-25 Large-scale sampling of C. elegans on E. coli HT115 RNAi-compatible bacteria Boelen, Rose Temmerman, Liesbet MicroPubl Biol New Methods As opposed to standard culturing, growing large numbers of C. elegans worms on Nematode Growth Medium-based RNA interference (RNAi) plates requires multiple transferring steps to prevent starvation, which increase handling time and reduce practical efficiency. We here provide an optimized method to grow four times more worms in RNAi conditions, thus saving on required resources and handling steps. Caltech Library 2023-01-09 /pmc/articles/PMC9871793/ /pubmed/36703717 http://dx.doi.org/10.17912/micropub.biology.000692 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| spellingShingle | New Methods Boelen, Rose Temmerman, Liesbet Large-scale sampling of C. elegans on E. coli HT115 RNAi-compatible bacteria |
| title |
Large-scale sampling of
C. elegans
on
E. coli
HT115 RNAi-compatible bacteria
|
| title_full |
Large-scale sampling of
C. elegans
on
E. coli
HT115 RNAi-compatible bacteria
|
| title_fullStr |
Large-scale sampling of
C. elegans
on
E. coli
HT115 RNAi-compatible bacteria
|
| title_full_unstemmed |
Large-scale sampling of
C. elegans
on
E. coli
HT115 RNAi-compatible bacteria
|
| title_short |
Large-scale sampling of
C. elegans
on
E. coli
HT115 RNAi-compatible bacteria
|
| title_sort | large-scale sampling of
c. elegans
on
e. coli
ht115 rnai-compatible bacteria |
| topic | New Methods |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871793/ https://www.ncbi.nlm.nih.gov/pubmed/36703717 http://dx.doi.org/10.17912/micropub.biology.000692 |
| work_keys_str_mv | AT boelenrose largescalesamplingofcelegansonecoliht115rnaicompatiblebacteria AT temmermanliesbet largescalesamplingofcelegansonecoliht115rnaicompatiblebacteria |