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Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level

Introduction: Aortic aneurysm is a life-threatening disease resulted from progressive dilatation of the aorta, which can be subdivided into thoracic and abdominal aortic aneurysms. Sustained subcutaneous angiotensin II infusion can induce aortic aneurysms in mice. However, the relevance of using ang...

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Autores principales: Wu, Hong, Xie, Cheng, Wang, Ruilin, Cheng, Jun, Xu, Qingbo, Zhao, Haige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871934/
https://www.ncbi.nlm.nih.gov/pubmed/36703732
http://dx.doi.org/10.3389/fphar.2022.1095757
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author Wu, Hong
Xie, Cheng
Wang, Ruilin
Cheng, Jun
Xu, Qingbo
Zhao, Haige
author_facet Wu, Hong
Xie, Cheng
Wang, Ruilin
Cheng, Jun
Xu, Qingbo
Zhao, Haige
author_sort Wu, Hong
collection PubMed
description Introduction: Aortic aneurysm is a life-threatening disease resulted from progressive dilatation of the aorta, which can be subdivided into thoracic and abdominal aortic aneurysms. Sustained subcutaneous angiotensin II infusion can induce aortic aneurysms in mice. However, the relevance of using angiotensin II induction model to study aneurysm disease and the degree of commonality between species remain elusive. Methods: We utilized scRNA-seq to infer aortic cell sub-structures and transcriptional profiles in clinical patient TAAs and AAAs, as well as mouse models of corresponding diseases (Ang II induction) and in healthy mouse aorta. Unbiased comparison between mice and humans explored the possible reasonability and utility of mouse Ang II-induced aortic aneurysm as a model for human aortic aneurysm diseases. Meanwhile, we performed comparative analysis of aortic aneurysms between TAA and AAA in both organisms. Results and Discussion: We demonstrated similarities and differences of changes in the components of human and mouse cell types, and our unbiased comparison between mouse and human identified well conserved subpopulations of SMCs and macrophages. Furthermore, the results of our comparative analyses suggested different biological functions and distinct potential pathogenic genes for thoracic and abdominal aortic aneurysms. MIF and SPP1 signaling networks participated in aortic aneurysm in both organisms. This study maps aortic aneurysm and offers opportunities for future researches to investigate the potential of subpopulations or marker genes as therapy targets.
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spelling pubmed-98719342023-01-25 Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level Wu, Hong Xie, Cheng Wang, Ruilin Cheng, Jun Xu, Qingbo Zhao, Haige Front Pharmacol Pharmacology Introduction: Aortic aneurysm is a life-threatening disease resulted from progressive dilatation of the aorta, which can be subdivided into thoracic and abdominal aortic aneurysms. Sustained subcutaneous angiotensin II infusion can induce aortic aneurysms in mice. However, the relevance of using angiotensin II induction model to study aneurysm disease and the degree of commonality between species remain elusive. Methods: We utilized scRNA-seq to infer aortic cell sub-structures and transcriptional profiles in clinical patient TAAs and AAAs, as well as mouse models of corresponding diseases (Ang II induction) and in healthy mouse aorta. Unbiased comparison between mice and humans explored the possible reasonability and utility of mouse Ang II-induced aortic aneurysm as a model for human aortic aneurysm diseases. Meanwhile, we performed comparative analysis of aortic aneurysms between TAA and AAA in both organisms. Results and Discussion: We demonstrated similarities and differences of changes in the components of human and mouse cell types, and our unbiased comparison between mouse and human identified well conserved subpopulations of SMCs and macrophages. Furthermore, the results of our comparative analyses suggested different biological functions and distinct potential pathogenic genes for thoracic and abdominal aortic aneurysms. MIF and SPP1 signaling networks participated in aortic aneurysm in both organisms. This study maps aortic aneurysm and offers opportunities for future researches to investigate the potential of subpopulations or marker genes as therapy targets. Frontiers Media S.A. 2023-01-10 /pmc/articles/PMC9871934/ /pubmed/36703732 http://dx.doi.org/10.3389/fphar.2022.1095757 Text en Copyright © 2023 Wu, Xie, Wang, Cheng, Xu and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Wu, Hong
Xie, Cheng
Wang, Ruilin
Cheng, Jun
Xu, Qingbo
Zhao, Haige
Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level
title Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level
title_full Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level
title_fullStr Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level
title_full_unstemmed Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level
title_short Comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level
title_sort comparative analysis of thoracic and abdominal aortic aneurysms across the segment and species at the single-cell level
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871934/
https://www.ncbi.nlm.nih.gov/pubmed/36703732
http://dx.doi.org/10.3389/fphar.2022.1095757
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