Antimicrobial Susceptibility Testing Performed in RPMI 1640 Reveals Azithromycin Efficacy against Carbapenem-Resistant Acinetobacter baumannii and Predicts In Vivo Outcomes in Galleria mellonella

Antimicrobial susceptibility testing (AST) in RPMI 1640, a more physiologically relevant culture medium, revealed that a substantial proportion of carbapenem-resistant Acinetobacter baumannii isolates were susceptible to azithromycin, a macrolide antibiotic not currently considered effective against...

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Autores principales: Miller, Sarah, Goy, Kristine, She, Rosemary, Spellberg, Brad, Luna, Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Susceptibility
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9872712/
https://www.ncbi.nlm.nih.gov/pubmed/36468875
http://dx.doi.org/10.1128/aac.01320-22
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author Miller, Sarah
Goy, Kristine
She, Rosemary
Spellberg, Brad
Luna, Brian
author_facet Miller, Sarah
Goy, Kristine
She, Rosemary
Spellberg, Brad
Luna, Brian
author_sort Miller, Sarah
collection PubMed
description Antimicrobial susceptibility testing (AST) in RPMI 1640, a more physiologically relevant culture medium, revealed that a substantial proportion of carbapenem-resistant Acinetobacter baumannii isolates were susceptible to azithromycin, a macrolide antibiotic not currently considered effective against A. baumannii. Experiments using Galleria mellonella validated these in vitro data. Our finding that RPMI 1640’s predictive accuracy for in vivo outcomes is superior to that of Mueller-Hinton II broth also supports the use of more physiologically relevant AST culturing conditions.
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spelling pubmed-98727122023-01-25 Antimicrobial Susceptibility Testing Performed in RPMI 1640 Reveals Azithromycin Efficacy against Carbapenem-Resistant Acinetobacter baumannii and Predicts In Vivo Outcomes in Galleria mellonella Miller, Sarah Goy, Kristine She, Rosemary Spellberg, Brad Luna, Brian Antimicrob Agents Chemother Susceptibility Antimicrobial susceptibility testing (AST) in RPMI 1640, a more physiologically relevant culture medium, revealed that a substantial proportion of carbapenem-resistant Acinetobacter baumannii isolates were susceptible to azithromycin, a macrolide antibiotic not currently considered effective against A. baumannii. Experiments using Galleria mellonella validated these in vitro data. Our finding that RPMI 1640’s predictive accuracy for in vivo outcomes is superior to that of Mueller-Hinton II broth also supports the use of more physiologically relevant AST culturing conditions. American Society for Microbiology 2022-12-05 /pmc/articles/PMC9872712/ /pubmed/36468875 http://dx.doi.org/10.1128/aac.01320-22 Text en Copyright © 2022 Miller et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Susceptibility
Miller, Sarah
Goy, Kristine
She, Rosemary
Spellberg, Brad
Luna, Brian
Antimicrobial Susceptibility Testing Performed in RPMI 1640 Reveals Azithromycin Efficacy against Carbapenem-Resistant Acinetobacter baumannii and Predicts In Vivo Outcomes in Galleria mellonella
title Antimicrobial Susceptibility Testing Performed in RPMI 1640 Reveals Azithromycin Efficacy against Carbapenem-Resistant Acinetobacter baumannii and Predicts In Vivo Outcomes in Galleria mellonella
title_full Antimicrobial Susceptibility Testing Performed in RPMI 1640 Reveals Azithromycin Efficacy against Carbapenem-Resistant Acinetobacter baumannii and Predicts In Vivo Outcomes in Galleria mellonella
title_fullStr Antimicrobial Susceptibility Testing Performed in RPMI 1640 Reveals Azithromycin Efficacy against Carbapenem-Resistant Acinetobacter baumannii and Predicts In Vivo Outcomes in Galleria mellonella
title_full_unstemmed Antimicrobial Susceptibility Testing Performed in RPMI 1640 Reveals Azithromycin Efficacy against Carbapenem-Resistant Acinetobacter baumannii and Predicts In Vivo Outcomes in Galleria mellonella
title_short Antimicrobial Susceptibility Testing Performed in RPMI 1640 Reveals Azithromycin Efficacy against Carbapenem-Resistant Acinetobacter baumannii and Predicts In Vivo Outcomes in Galleria mellonella
title_sort antimicrobial susceptibility testing performed in rpmi 1640 reveals azithromycin efficacy against carbapenem-resistant acinetobacter baumannii and predicts in vivo outcomes in galleria mellonella
topic Susceptibility
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9872712/
https://www.ncbi.nlm.nih.gov/pubmed/36468875
http://dx.doi.org/10.1128/aac.01320-22
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