Cargando…

Approaches for the isolation and long-term expansion of pericytes from human and animal tissues

Pericytes surround capillaries in every organ of the human body. They are also present around the vasa vasorum, the small blood vessels that supply the walls of larger arteries and veins. The clinical interest in pericytes is rapidly growing, with the recognition of their crucial roles in controllin...

Descripción completa

Detalles Bibliográficos
Autores principales: Alvino, Valeria Vincenza, Mohammed, Khaled Abdelsattar Kassem, Gu, Yue, Madeddu, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873410/
https://www.ncbi.nlm.nih.gov/pubmed/36704463
http://dx.doi.org/10.3389/fcvm.2022.1095141
_version_ 1784877590058631168
author Alvino, Valeria Vincenza
Mohammed, Khaled Abdelsattar Kassem
Gu, Yue
Madeddu, Paolo
author_facet Alvino, Valeria Vincenza
Mohammed, Khaled Abdelsattar Kassem
Gu, Yue
Madeddu, Paolo
author_sort Alvino, Valeria Vincenza
collection PubMed
description Pericytes surround capillaries in every organ of the human body. They are also present around the vasa vasorum, the small blood vessels that supply the walls of larger arteries and veins. The clinical interest in pericytes is rapidly growing, with the recognition of their crucial roles in controlling vascular function and possible therapeutic applications in regenerative medicine. Nonetheless, discrepancies in methods used to define, isolate, and expand pericytes are common and may affect reproducibility. Separating pure pericyte preparations from the continuum of perivascular mesenchymal cells is challenging. Moreover, variations in functional behavior and antigenic phenotype in response to environmental stimuli make it difficult to formulate an unequivocal definition of bona fide pericytes. Very few attempts were made to develop pericytes as a clinical-grade product. Therefore, this review is devoted to appraising current methodologies’ pros and cons and proposing standardization and harmonization improvements. We highlight the importance of developing upgraded protocols to create therapeutic pericyte products according to the regulatory guidelines for clinical manufacturing. Finally, we describe how integrating RNA-seq techniques with single-cell spatial analysis, and functional assays may help realize the full potential of pericytes in health, disease, and tissue repair.
format Online
Article
Text
id pubmed-9873410
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98734102023-01-25 Approaches for the isolation and long-term expansion of pericytes from human and animal tissues Alvino, Valeria Vincenza Mohammed, Khaled Abdelsattar Kassem Gu, Yue Madeddu, Paolo Front Cardiovasc Med Cardiovascular Medicine Pericytes surround capillaries in every organ of the human body. They are also present around the vasa vasorum, the small blood vessels that supply the walls of larger arteries and veins. The clinical interest in pericytes is rapidly growing, with the recognition of their crucial roles in controlling vascular function and possible therapeutic applications in regenerative medicine. Nonetheless, discrepancies in methods used to define, isolate, and expand pericytes are common and may affect reproducibility. Separating pure pericyte preparations from the continuum of perivascular mesenchymal cells is challenging. Moreover, variations in functional behavior and antigenic phenotype in response to environmental stimuli make it difficult to formulate an unequivocal definition of bona fide pericytes. Very few attempts were made to develop pericytes as a clinical-grade product. Therefore, this review is devoted to appraising current methodologies’ pros and cons and proposing standardization and harmonization improvements. We highlight the importance of developing upgraded protocols to create therapeutic pericyte products according to the regulatory guidelines for clinical manufacturing. Finally, we describe how integrating RNA-seq techniques with single-cell spatial analysis, and functional assays may help realize the full potential of pericytes in health, disease, and tissue repair. Frontiers Media S.A. 2023-01-10 /pmc/articles/PMC9873410/ /pubmed/36704463 http://dx.doi.org/10.3389/fcvm.2022.1095141 Text en Copyright © 2023 Alvino, Mohammed, Gu and Madeddu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Alvino, Valeria Vincenza
Mohammed, Khaled Abdelsattar Kassem
Gu, Yue
Madeddu, Paolo
Approaches for the isolation and long-term expansion of pericytes from human and animal tissues
title Approaches for the isolation and long-term expansion of pericytes from human and animal tissues
title_full Approaches for the isolation and long-term expansion of pericytes from human and animal tissues
title_fullStr Approaches for the isolation and long-term expansion of pericytes from human and animal tissues
title_full_unstemmed Approaches for the isolation and long-term expansion of pericytes from human and animal tissues
title_short Approaches for the isolation and long-term expansion of pericytes from human and animal tissues
title_sort approaches for the isolation and long-term expansion of pericytes from human and animal tissues
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873410/
https://www.ncbi.nlm.nih.gov/pubmed/36704463
http://dx.doi.org/10.3389/fcvm.2022.1095141
work_keys_str_mv AT alvinovaleriavincenza approachesfortheisolationandlongtermexpansionofpericytesfromhumanandanimaltissues
AT mohammedkhaledabdelsattarkassem approachesfortheisolationandlongtermexpansionofpericytesfromhumanandanimaltissues
AT guyue approachesfortheisolationandlongtermexpansionofpericytesfromhumanandanimaltissues
AT madeddupaolo approachesfortheisolationandlongtermexpansionofpericytesfromhumanandanimaltissues