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Approaches for the isolation and long-term expansion of pericytes from human and animal tissues
Pericytes surround capillaries in every organ of the human body. They are also present around the vasa vasorum, the small blood vessels that supply the walls of larger arteries and veins. The clinical interest in pericytes is rapidly growing, with the recognition of their crucial roles in controllin...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873410/ https://www.ncbi.nlm.nih.gov/pubmed/36704463 http://dx.doi.org/10.3389/fcvm.2022.1095141 |
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author | Alvino, Valeria Vincenza Mohammed, Khaled Abdelsattar Kassem Gu, Yue Madeddu, Paolo |
author_facet | Alvino, Valeria Vincenza Mohammed, Khaled Abdelsattar Kassem Gu, Yue Madeddu, Paolo |
author_sort | Alvino, Valeria Vincenza |
collection | PubMed |
description | Pericytes surround capillaries in every organ of the human body. They are also present around the vasa vasorum, the small blood vessels that supply the walls of larger arteries and veins. The clinical interest in pericytes is rapidly growing, with the recognition of their crucial roles in controlling vascular function and possible therapeutic applications in regenerative medicine. Nonetheless, discrepancies in methods used to define, isolate, and expand pericytes are common and may affect reproducibility. Separating pure pericyte preparations from the continuum of perivascular mesenchymal cells is challenging. Moreover, variations in functional behavior and antigenic phenotype in response to environmental stimuli make it difficult to formulate an unequivocal definition of bona fide pericytes. Very few attempts were made to develop pericytes as a clinical-grade product. Therefore, this review is devoted to appraising current methodologies’ pros and cons and proposing standardization and harmonization improvements. We highlight the importance of developing upgraded protocols to create therapeutic pericyte products according to the regulatory guidelines for clinical manufacturing. Finally, we describe how integrating RNA-seq techniques with single-cell spatial analysis, and functional assays may help realize the full potential of pericytes in health, disease, and tissue repair. |
format | Online Article Text |
id | pubmed-9873410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98734102023-01-25 Approaches for the isolation and long-term expansion of pericytes from human and animal tissues Alvino, Valeria Vincenza Mohammed, Khaled Abdelsattar Kassem Gu, Yue Madeddu, Paolo Front Cardiovasc Med Cardiovascular Medicine Pericytes surround capillaries in every organ of the human body. They are also present around the vasa vasorum, the small blood vessels that supply the walls of larger arteries and veins. The clinical interest in pericytes is rapidly growing, with the recognition of their crucial roles in controlling vascular function and possible therapeutic applications in regenerative medicine. Nonetheless, discrepancies in methods used to define, isolate, and expand pericytes are common and may affect reproducibility. Separating pure pericyte preparations from the continuum of perivascular mesenchymal cells is challenging. Moreover, variations in functional behavior and antigenic phenotype in response to environmental stimuli make it difficult to formulate an unequivocal definition of bona fide pericytes. Very few attempts were made to develop pericytes as a clinical-grade product. Therefore, this review is devoted to appraising current methodologies’ pros and cons and proposing standardization and harmonization improvements. We highlight the importance of developing upgraded protocols to create therapeutic pericyte products according to the regulatory guidelines for clinical manufacturing. Finally, we describe how integrating RNA-seq techniques with single-cell spatial analysis, and functional assays may help realize the full potential of pericytes in health, disease, and tissue repair. Frontiers Media S.A. 2023-01-10 /pmc/articles/PMC9873410/ /pubmed/36704463 http://dx.doi.org/10.3389/fcvm.2022.1095141 Text en Copyright © 2023 Alvino, Mohammed, Gu and Madeddu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cardiovascular Medicine Alvino, Valeria Vincenza Mohammed, Khaled Abdelsattar Kassem Gu, Yue Madeddu, Paolo Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
title | Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
title_full | Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
title_fullStr | Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
title_full_unstemmed | Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
title_short | Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
title_sort | approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
topic | Cardiovascular Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873410/ https://www.ncbi.nlm.nih.gov/pubmed/36704463 http://dx.doi.org/10.3389/fcvm.2022.1095141 |
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